Isozyme analysis of seedling samples in some species of Hyoscyamus from Iran

Sharifi, Golandam; Kouhsari, Shideh Montasser; Ebrahimzadeh, Hassan; Khatamsaz, M

doi:10.3923/pjbs.2006.1685.1692

Cited by 2 publications

(2 citation statements)

References 32 publications

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“…They also act as; 'nature's biological modifiers' as antiinflammatory, anti-allergens and provocative second-phase enzymes that remove carcinogens and mutagens (Ogunwenmo et al 2007;Ngoci et al 2011). In addition, the presence of superoxide dismutase and peroxidase in Hyoscyamus insanus (Sharifi et al 2006) can also contribute to its effectiveness as an antioxidant.…”

Section: Hydrogen Peroxide Assaymentioning

confidence: 99%

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Phytochemical Assessment, Total Phenolic Content, Cytotoxic, Antioxidant and Antidiabetic Activities of Hyoscyamus Insanus

Shah

Rahmat

Ahmed

2021

J. Chil. Chem. Soc.

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Hyoscyamus insanus (family Solanaceae) was traditionally used for treatment of asthma, relieving pain and increasing female body weight. The present project was intended to assess the phytochemicals, total phenolic content, cytotoxic, antioxidant and antidiabetic activities of Hyoscyamus insanus leaves methanolic extract and its special fractions. After extraction, the methanolic extract was sequentially fractionated with n-hexane, chloroform and water followed by phytochemicals analysis of each sample. Brine shrimp lethality bioassay, Folin-Ciocalteu phenol reagent, DPPH, ABTS •+ , H2O2 and alpha-amylase inhibition assays were employed for the assessment of cytotoxic property, total phenolic content, antioxidant and anti-diabetic potency of methanolic extract and its fractions. Amino acids and protein, carbohydrates, flavonoids and saponins were found in the methanolic extract and aqueous fraction while chloroform fraction exhibited carbohydrates, flavonoids and saponins. The highest cytotoxic activity (80.6±1.2%) was exhibited by methanolic extract while maximum total phenolic content (21.93±1.17 mg GAE/g) were found in the chloroform fraction. The employed antioxidant assays expressed different results i.e. methanolic extract demonstrated highest; 83.99%, 74.19% and 51.15% free radical scavenging characteristics in ABTS, DPPH and H2O2 assays respectively. The methanolic extract showed significant (53.44%) antidiabetic properties. The correlation of total phenolic content with percentage antioxidant and anti-diabetic capabilities of methanolic extract and its special fractions was observed to be non-significant (P > 0.05). It is concluded that the Hyoscyamus insanus is a potential source of phenolic, cytotoxic, antioxidant and antidiabetic compounds.

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Section: Hydrogen Peroxide Assaymentioning

confidence: 99%

“…The main alkaloids, Hyoscyamine (atropine), hyoscine, and apoatropine were found in it (Tafaghodi & Rahimizadeh 2003). The existence of some enzymes, peroxidase, superoxide dismutase, and malate dehydrogenase in it has been documented (Sharifi et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Assessment, Total Phenolic Content, Cytotoxic, Antioxidant and Antidiabetic Activities of Hyoscyamus Insanus

Shah

Rahmat

Ahmed

2021

J. Chil. Chem. Soc.

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Isozymatic Characterization of Accessions of Arundinaria falcata (Nees)

Tiwari

Bakshi

2015

Forest Res

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Bamboos are arborescent grasses belonging to the family Poaceae. To study the genetics of bamboo specially, one has to face enormous difficulties such as rare flowering and we cannot make hybridizations as easily as like other plant taxa. Unlike other tropical bamboos, studies on hill bamboos are scarce. Arundinaria falcata, an important hill bamboo, acts as soil stabilizer in hilly terrain. This study assessed the genetic diversity of 10 accessions of A. falcata collected from different localities of Garhwal Himalayas (India) and established at Hill bamboo Germplasm at Khirsu (India), using isozyme marker with four enzyme system (peroxidase, esterase, malate dehydrogenase and malic enzyme). Isozymatic analyses were performed with polyacrylamide gels (one system), bands were scored as binary data. Cluster analyses were conducted, using Jaccard´s similarity coefficient and UPGMA method. Very high degree of similarity was reported i.e. 63-94% among different accessions. Dendrogram revealed two major clusters with three (A8-A10) and seven (A1-A7) accessions respectively. The results obtained inferred low genetic diversity in the species and urgent need of the in situ conservation of the natural genetic resources of the A. falcata species.

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Isozyme analysis of seedling samples in some species of Hyoscyamus from Iran

Cited by 2 publications

References 32 publications

Phytochemical Assessment, Total Phenolic Content, Cytotoxic, Antioxidant and Antidiabetic Activities of Hyoscyamus Insanus

Phytochemical Assessment, Total Phenolic Content, Cytotoxic, Antioxidant and Antidiabetic Activities of Hyoscyamus Insanus

Isozymatic Characterization of Accessions of Arundinaria falcata (Nees)

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