Isovolemic hemodilution and skeletal muscle function during ischemia and reperfusion

Frost‐Arner, Lieselotte; Wellander, Erik; Mattsson, Erney; Bergqvist, David

doi:10.1002/(sici)1098-2752(1998)18:2<79::aid-micr4>3.3.co;2-z

Cited by 1 publication

(2 citation statements)

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“…In a canine gracilis study a graded response to ischaemia and reperfusion was documented: three hours ischaemia 2% necrosis, four hours ischaemia 30%, and ve hours ischaemia 90% necrosis (18). Post-ischaemic muscle strength in rabbits shows the same pattern as the recovery of energy metabolites and extent of necrosis after various ischaemic times (8). After a period of 2.5 hours ischaemia and 90 minutes of reperfusion the strength recovered to 80%, but when the ischaemic time exceeded three to four hours the muscle strength dropped dramatically (8).…”

Section: Discussionmentioning

confidence: 67%

“…Post-ischaemic muscle strength in rabbits shows the same pattern as the recovery of energy metabolites and extent of necrosis after various ischaemic times (8). After a period of 2.5 hours ischaemia and 90 minutes of reperfusion the strength recovered to 80%, but when the ischaemic time exceeded three to four hours the muscle strength dropped dramatically (8). The deterioration seems to start between three to four hours of ischaemia and it therefore seems that revascularisation should take place within three hours of normothermic ischaemia if good function and quality of the muscle are required.…”

Section: Discussionmentioning

confidence: 99%

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Energy Metabolism During Microsurgical Transfer of Human Skeletal Muscle Assessed by High-Pressure Liquid Chromatography and by 31 P-Nuclear Magnetic Resonance

Lundberg

Elander

Rakotonirainy

et al. 2002

Scandinavian Journal of Plastic and Reconstructive Surgery and

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The effect of ischaemia and reperfusion on human skeletal muscle was studied during free vascularised muscle transfer. Muscle biopsy specimens were taken from patients having microsurgical muscle transfer, 18 cases (17 patients; 12 men, 5 women). The biopsies were taken three times: before transfer of the muscle (control), at maximum ischaemic time, and one hour after revascularisation. The biopsy specimens were analysed for purine nucleotides, by high-pressure liquid chromatography (HPLC), and by nuclear magnetic resonance (NMR) at 500 MHz. Phosphocreatine (PCr) recovered only partially (79%) and adenosine triphosphate (ATP) did not differ significantly from normal control after revascularisation and a mean ischaemic time of 114 minutes. NMR measurements showed an accumulation of glucose-6-phosphate (G-6-P) during the ischaemic period, indicating anaerobic metabolism. After three hours of ischaemia and one hour of reperfusion the PCr recovery was less than 60% (r = 0.7). The results confirm those of previous animal studies, which set three hours normothermic ischaemia as a safe limit for tissue preservation when transferring skeletal muscle. Longer ischaemic times may cause serious postoperative healing problems and reduced muscle function.

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Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 99%