Isotype-specific serum antibody responses of sheep to Haemonchus contortus antigens

Schallig, Henk D. F. H.; Leeuwen, M.A. van; Hendrikx, W.M.L.

doi:10.1016/0304-4017(94)00675-3

Cited by 45 publications

(34 citation statements)

References 30 publications

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“…But in contrast to studies in Texel and Castellana sheep showing a stronger IgG and IgA response in animals previously exposed to infection (Schallig et al, 1995;Gomez-Munoz et al, 1999), in Creole kids naturally parasitized on pasture before the experimental infection, a weak response was observed except for IgA anti-ESP which showed amounts similar to primo-infected sheep. Previous studies in sheep suggest that IgA may be the major mechanism controlling fecundity of H. contortus and worm length and fecundity of Teladorsagia cicumcincta (Stear et al, 1995b;Strain et al, 2002 (Amarante et al, 2005).…”

Section: Discussioncontrasting

confidence: 96%

Serum antibody responses in Creole kids experimentally infected with Haemonchus contortus

Bambou¹,

Chevrotière²,

Varo³

et al. 2008

Veterinary Parasitology

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The objective of this study was to evaluate the relationship of parasite-specific serum antibodies with the resistance status of Creole kids. The average breeding values on egg output predicted in a context of natural infection at 11 months of age were distant of 1.07 genetic standard deviation between resistant and susceptible animals. After drenching the animals were maintained worm-free during 1 month until experimental infection with 10,000 Haemonchus contortus infective larvae (L3). Enzyme-linked immunosorbent assay was carried out in serum samples to determine the level of IgG, IgA and IgE anti-H. contortus L3 crude extracts and adult excretion/secretion products (ESP). Parasitological and blood immunological parameters were measured on the 2 extreme groups. Despite the absence of any typical signs of haemonchosis, susceptible kids had more than 11 times higher faecal egg counts (FEC) at 35 days post-infection (d.p.i.) than resistant kids had. Levels of immunoglobulin against H. contortus L3 and ESP increased significantly after infection in both groups. However, no difference in the host immune response mediated by immunoglobulin against H. contortus was evidenced between groups. This finding suggests that, in goats previously infected by H. contortus, a degree of protection occurred and the phenotypic and genetic segregation in resistant and susceptible animals were not related to the humoral immune response. The correlation coefficients between FEC and IgE anti-ESP (r = 0.593; P < 0.05 was significant in both resistant and susceptible animals. Such correlation suggesting a hypersensitivity reaction dependent on worm prolificacy has never been described. This result needs further studies to understand the mechanisms underlying this observation. #

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Section: Discussioncontrasting

confidence: 96%

Serum antibody responses in Creole kids experimentally infected with Haemonchus contortus

Bambou¹,

Chevrotière²,

Varo³

et al. 2008

Veterinary Parasitology

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“…The development of immunity to gastrointestinal (GI) parasitic infections appears to depend on the host's ability to mobilise both cellular and humoral immune effectors locally at the site of infection and systemically in the blood circulation. Haemonchus contortus infestation in sheep is known to elicit a Th2-polarised immune response characterised by Th2 cytokines (Lacroux et al, 2006), the recruitment of higher number of eosinophils, mast cells and globule leucocytes (Balic et al, 2000(Balic et al, , 2006 and the development of local and circulating antibody responses (Schallig et al, 1995;Gòmez-Muñoz et al, 1999).…”

Section: Extrinsic Factors the Environmentmentioning

confidence: 99%

“…The proximal most likely location of this QTL corresponded to the IL-3/IL-4/IL-5 region. As these cytokines are characteristic of Th2 type immune responses (Lacroux et al, 2006), studies of both the cellular and humoral responses will be of paramount importance in the understanding of the mechanism of resistance (Gill, 1991;Schallig et al, 1995;Colditz et al, 1996;Balic et al, 2000). Hence, there is still much to be done to understand the mechanisms underlying differences both between and within breeds of sheep in terms of their resistance to helminth infections in order to identify the best markers of resistance for use during the selection processes.…”

Section: Breeding For Resistancementioning

confidence: 99%

Trends and challenges in the effective and sustainable control ofHaemonchus contortusinfection in sheep. Review

2007

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Summary :Haemonchosis, with its very wide distribution, has become a very important production constraint in sheep farms in tropical, subtropical and temperate regions worldwide. Various intrinsic and extrinsic factors determine the survival of Haemonchus contortus and hence the development of the disease in the animal. In general, control of gastrointestinal nematode infestation in sheep relies heavily on anthelmintic treatments. However, the indiscriminate use of these drugs has led to the widespread emergence of drug resistant strains of parasites, that has necessitated the development and use of various parasite control methods such as grazing management, biological agents and vaccines and the selection of resistant breeds of animals, with or without moderate use of anthelmintics. The ultimate goal of such control programs is to enhance productivity, while minimising risks regarding drug resistance and consumer and environmental concerns. This review attempts to highlight the different methods employed in the control of haemonchosis in sheep and the practical limitations associated with both control programs and the internal and external factors associated with the parasite and its microenvironment. Résumé : MOYENS DE LUTTE CONTRE HAEMONCHUS CONTORTUS

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“…2). Uninfected sheep sera (lanes [21][22][23][24][25][26][27][28][29][30] showed no clear 26-kD recognition. Figure 3 shows the results obtained when the Hc26-enriched fraction was tested with pooled sera of infected (lanes 1) and uninfected animals (lanes 0).…”

Section: Diagnostic Value Of A41 To Test H Contortus Infections In Smentioning

confidence: 99%

Serodiagnosis of Haemonchosis with a Somatic Antigen (Hc26) in Several Breeds of Sheep

et al. 2000

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Abstract. Sera from 53 sheep belonging to Castellano, Churro, Manchego, and Merino breeds were analyzed to test the diagnostic value of a 26-kD antigen from adult Haemonchus contortus at prepatency and early and late patency of experimental haemonchosis. Animals that received zero, 1, or 2 infections with the parasite were tested. In addition, sera from 20 experimentally infected and 10 noninfected Texel sheep were used to test the antigen. Sera from 37 infected animals at prepatency as well as at patency in primary and secondary infection were found positive with the 26-kD antigen. However, sera from 10 animals with the lowest worm burdens (second infection) did not recognize the antigen during early patency (day 28 postinfection). IgG1 was the only isotype implicated in antigen recognition because IgG2, IgA, and IgM, in the same sera, showed no reactivity with the peptide. Antigen specificity was confirmed because hyperimmune sera against infective larvae and adult stages of the most common gastrointestinal nematodes found in natural infections in sheep (Trichostrongylus colubriformis and Teladorsagia circumcincta) did not recognize this peptide. The antigen was recognized only by anti-adult H. contortus hyperimmune sera and appeared to be absent in the L3 parasite stage. In addition, the partial N-terminal amino acid sequence of the diagnostic peptide is reported.Sheep haemonchosis is a widespread parasitic disease in the temperate areas of the world. Diagnosis is based on coprological methods, which are time consuming and require specially trained personnel. A rapid and simple test with which a considerable number of samples could be processed at the same time would be advantageous. Circulating antigens could not be detected in infected animals, 22 and the assays developed to detect parasite antigens in the feces of infected animals, although occasionally able to detect surface antigens of the parasite, were not always related to the parasite burden. 10 In addition, methods used to detect specific DNA are restricted to patency and are expensive to use routinely. 6,23 The detection of specific serum antibodies against the parasite by ELISA has produced inconsistent results because of the complex nature of the antigen extract used 5 and limited value of the soluble extracts as an antigen source. 21 In addition, cross-antigenicity has been widely described among related and unrelated nematodes. 1,7,9,27,28 The use of less complex antigen mixtures, such as excretory/secretory products, has led to better results in ELISA tests, 24 but there is still some cross-reactivity between related nematodes, both in ELISA and in Western blotting. 25 From the Departamento de Patología Animal I, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain (Gó-mez-Muñoz, Domínguez, Fernández-Pérez, Méndez, de la Fuente, Alunda, Cuquerella), and the Servicio Territorial de Agricultura y Ganadería, Medina de Rioseco, 47800 Valladolid, Spain (Gómez-Iglesias).Received for publication July 31, 1998.A partially ch...

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Isotype-specific serum antibody responses of sheep to Haemonchus contortus antigens

Cited by 45 publications

References 30 publications

Serum antibody responses in Creole kids experimentally infected with Haemonchus contortus

Serum antibody responses in Creole kids experimentally infected with Haemonchus contortus

Trends and challenges in the effective and sustainable control ofHaemonchus contortusinfection in sheep. Review

Serodiagnosis of Haemonchosis with a Somatic Antigen (Hc26) in Several Breeds of Sheep

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