Isotropically Etched Silicon Microarrays for Rapid Breast Cancer Cell Capture

Nikkhah, Mehdi; Strobl, Jeannine S.; Srinivasaraghavan, Vaishnavi; Agah, Masoud

doi:10.1109/jsen.2012.2227716

Cited by 12 publications

(10 citation statements)

References 42 publications

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“…They were originally developed for polymerase chain reaction (PCR) 30 31 . Various planar microreactor array type platforms have also been developed for PCR 32 33 34 35 36 37 38 39 40 41 42 43 and other life science applications 44 45 46 47 48 49 50 51 52 but none use the unique microfluidic configuration presented here. Cell-free in vitro protein expression has been done in various microreactor formats 53 54 55 56 57 58 59 but never using the present configuration for filling and sealing the microreactors.…”

Section: Discussionmentioning

confidence: 99%

Microreactor Array Device

Wiktor

Brunner

Kahn

et al. 2015

Sci Rep

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We report a device to fill an array of small chemical reaction chambers (microreactors) with reagent and then seal them using pressurized viscous liquid acting through a flexible membrane. The device enables multiple, independent chemical reactions involving free floating intermediate molecules without interference from neighboring reactions or external environments. The device is validated by protein expressed in situ directly from DNA in a microarray of ~10,000 spots with no diffusion during three hours incubation. Using the device to probe for an autoantibody cancer biomarker in blood serum sample gave five times higher signal to background ratio compared to standard protein microarray expressed on a flat microscope slide. Physical design principles to effectively fill the array of microreactors with reagent and experimental results of alternate methods for sealing the microreactors are presented.

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Section: Discussionmentioning

confidence: 99%

Microreactor Array Device

Wiktor

Brunner

Kahn

et al. 2015

Sci Rep

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“…Etching is commonly used to fabricate wells on the surfaces of fiber optic filaments or silicone substrates. Chemical etching has also been utilized to produce microwells on the surface of optical fibers [77], [78], while deep reactive ion etching produces concave microwells on silicone [79], [80]. Additionally, injection molding has been employed to fabricate microwells made of polystyrene [81].…”

Section: Fabrication Of Microwellsmentioning

confidence: 99%

Fabrication Of Sieved-Microwells For Single Particle Capture

Romita¹

2021

Preprint

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<div>The use of microwells is popular for a wide range of applications due to its’ ease of use. However, the seeding of conventional microwells, which are closed at the bottom, is restricted to gravitational sedimentation for cell or particle deposition and therefore require lengthy settling times to maximize well occupancy. Microfluidics has accelerated cell or particle capture through flow but is mostly limited to gravitationally-driven settling for capture into the wells. An alternative approach to conventional closed-microwells, sieved microwells supersedes reliance on gravity by using hydrodynamic forces through the open pores at the bottom of the microwells to draw targets into the wells. The aim of this thesis is to develop a rapid and high-throughput fabrication method for sieved microwells and integrate the microwells into a double-layered microfluidic device to enable crossflow trapping. The resulting device achieves an 87% well occupancy in under 10 seconds.</div>

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“…F I G U R E 2 Different microwell geometries and fabrication techniques: A, SEM image of cylindrical microwells fabricated using soft lithography, reprinted, with permission, from Lin et al [76] ; B, SEM image of conical microwells fabricated using reactive ion etching, reprinted, with permission, from Nikkah et al [77] ; C, D, photomicrography of cubic microwells fabricated using soft lithography, reprinted, with permission, from Gong et al and Sinkala and Eddington [78,79] ; and E, F, photomicrography of triangular-prism microwells created using standard soft lithography technique, reprinted, with permission, from Park et al and Hattori et al [80,81] T A B L E 1 Types of materials used for microwell fabrications Dahlmann et al [85] Gong et al [78] Tang et al [93] Hydrogel Barrila et al [2] Antoni et al [94] Stock et al [95] Liu et al [96] Freed et al [97] Hutmacher [98] Marler et al [99] Poly(ethylene glycol) (PEG) Moeller et al [49] Poly(dimethyl siloxane) (PDMS)…”

Section: Squarementioning

confidence: 99%

“…Different microwell geometries and fabrication techniques: A, SEM image of cylindrical microwells fabricated using soft lithography, reprinted, with permission, from Lin et al [ 76 ] ; B, SEM image of conical microwells fabricated using reactive ion etching, reprinted, with permission, from Nikkah et al [ 77 ] ; C, D, photomicrography of cubic microwells fabricated using soft lithography, reprinted, with permission, from Gong et al and Sinkala and Eddington [ 78,79 ] ; and E, F, photomicrography of triangular‐prism microwells created using standard soft lithography technique, reprinted, with permission, from Park et al and Hattori et al [ 80,81 ] …”

Section: Optimization Of Microwell Design Parametersmentioning

confidence: 99%

A review on microwell and microfluidic geometric array fabrication techniques and its potential applications in cellular studies

2020

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The ability to trap precise quantities of cells or particles into confined areas has numerous applications for biological purposes. In particular, single cell trapping has received considerable attention because it permits the study of heterogeneity in a population, while trapping larger groups of cells have been used to form aggregates. Among several methods, the use of microwell offers a simple platform to capture cells or particles using hydrodynamic forces. This review examines the use of microwells in both static and microfluidic environments, and the application of microfluidic geometric arrays for trapping. This paper discusses the design and fabrication methods of microwells and compares the trapping and release techniques used in both static and microfluidicsintegrated microwells. Finally, we will summarize novel microfluidic geometric arrays used to capture cells or particles through hydrodynamic trapping.

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Isotropically Etched Silicon Microarrays for Rapid Breast Cancer Cell Capture

Cited by 12 publications

References 42 publications

Microreactor Array Device

Microreactor Array Device

Fabrication Of Sieved-Microwells For Single Particle Capture

A review on microwell and microfluidic geometric array fabrication techniques and its potential applications in cellular studies

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