Abstract:Variations in the isotopic composition of Zn present in various biological materials were determined using high-resolution multicollector inductively coupled plasma mass spectrometry (MC-ICPMS), following digestion and purification by anion exchange chromatography. To correct for differences in instrumental mass discrimination effects between samples and standards, Cu was employed as an elemental spike. Complementary analyses of Zn separates by sector field ICPMS instruments revealed that the concentrations of… Show more
“…This result proved that Zn isotopic fractionation occurred in the human body. Moreover, this lower value was consistent with the Zn isotopic data reported by Stenberg et al 12 These results demonstrate that Zn isotopic 427 ANALYTICAL SCIENCES APRIL 2005, VOL. 21 Zn data for RBC and hair samples: a, male; A, female; f, hair.…”
Section: Isotopic Heterogeneity Of Zn In the Human Bodysupporting
confidence: 91%
“…[1][2][3][4][5][6][7][8][9][10][11][12] While the mechanisms of isotopic fractionation are not yet well understood, biological activity appears to be particularly important for the cause of variations in isotopic composition. Zinc is one of the most important elements as a biological essential element to activate physiological functions of various enzymes; deficiency of this element leads to poor growth, dermatitis or impaired immune function.…”
Section: Introductionmentioning
confidence: 99%
“…More recently, precise Zn isotopic data on several biological materials such as bovine muscle, liver, blood or human hair demonstrated the wide range of variation in Zn isotopic ratios covering almost 1‰ among the samples. 12 However, neither variation among individuals nor seasonal change of the Zn isotopic composition has been investigated. In this study, in order to test the possible isotopic variations of Zn among the individuals and the seasonal change in isotopic compositions of Zn in a human body, isotopic analyses have been made on Zn in red blood cell (RBC) samples collected from five volunteers and a series of twelve RBC monthly-based samples collected over a period of one year.…”
“…This result proved that Zn isotopic fractionation occurred in the human body. Moreover, this lower value was consistent with the Zn isotopic data reported by Stenberg et al 12 These results demonstrate that Zn isotopic 427 ANALYTICAL SCIENCES APRIL 2005, VOL. 21 Zn data for RBC and hair samples: a, male; A, female; f, hair.…”
Section: Isotopic Heterogeneity Of Zn In the Human Bodysupporting
confidence: 91%
“…[1][2][3][4][5][6][7][8][9][10][11][12] While the mechanisms of isotopic fractionation are not yet well understood, biological activity appears to be particularly important for the cause of variations in isotopic composition. Zinc is one of the most important elements as a biological essential element to activate physiological functions of various enzymes; deficiency of this element leads to poor growth, dermatitis or impaired immune function.…”
Section: Introductionmentioning
confidence: 99%
“…More recently, precise Zn isotopic data on several biological materials such as bovine muscle, liver, blood or human hair demonstrated the wide range of variation in Zn isotopic ratios covering almost 1‰ among the samples. 12 However, neither variation among individuals nor seasonal change of the Zn isotopic composition has been investigated. In this study, in order to test the possible isotopic variations of Zn among the individuals and the seasonal change in isotopic compositions of Zn in a human body, isotopic analyses have been made on Zn in red blood cell (RBC) samples collected from five volunteers and a series of twelve RBC monthly-based samples collected over a period of one year.…”
“…Fig. 4Compilation of published δ 66/64 Zn values for animal tissue and the reference materials analysed in this study (squares, except BCR-639), reported relative to IRMM-3702 Zn [16, 18, 21, 22, 25, 26, 29, 60–62]. Literature data reported relative to JMC Lyon Zn was corrected for an offset of 0.32‰ between IRMM-3702 Zn and JMC Lyon Zn (Table 1)
…”
Biological reference materials with well-characterised stable isotope compositions are lacking in the field of ‘isotope biochemistry’, which seeks to understand bodily processes that rely on essential metals by determining metal stable isotope ratios. Here, we present Zn stable isotope data for six biological reference materials with certified trace metal concentrations: fish muscle, bovine muscle, pig kidney, human hair, human blood serum and human urine. Replicate analyses of multiple aliquots of each material achieved reproducibilities (2sd) of 0.04–0.13‰ for δ66/64Zn (which denotes the deviation of the 66Zn/64Zn ratio of a sample from a pure Zn reference material in parts per 1000). This implies only very minor isotopic heterogeneities within the samples, rendering them suitable as quality control materials for Zn isotope analyses. This endorsement is reinforced by (i) the close agreement of our Zn isotope data for two of the samples (bovine muscle and human blood serum) to previously published results for different batches of the same material and (ii) the similarity of the isotopic data for the samples (δ66/64Zn ≈ –0.8 to 0.0‰) to previously published Zn isotope results for similar biological materials. Further tests revealed that the applied Zn separation procedure is sufficiently effective to enable accurate data acquisition even at low mass resolving power (M/ΔM ≈ 400), as measurements and analyses conducted at much higher mass resolution (M/ΔM ≈ 8500) delivered essentially identical results.Electronic supplementary materialThe online version of this article (doi:10.1007/s00216-017-0240-y) contains supplementary material, which is available to authorized users.
“…To test this, we measured a suite of peat and plant samples spiked with NBS Cu 976 (certified ratio for 65 Cu/ 63 Cu is 0.4456 ± 0.0021, (Shields et al, 1964)) and corrected the instrumental bias using the external normalization approach (Maréchal et al, 1999;Ohno et al, 2005;Stenberg et al, 2004).…”
Section: Instrumentation and Measurement Protocolsmentioning
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