Isotopic Studies on 2′-Deoxyinosine-8-C14 Formation From Inosine-8-C14 by Washed Cell Suspention of Vitamin B12-Requiring Strain of Lactobacillus

Abstract

The authors (1) have reported the formation of deoxyinosine from inosine by cell washed suspension of Lactobacillus leichmannii ATCC 7830. Similar studies were further made using inosine-8-C14 and the washed cell suspension of the strain. First, the isolation and identification of the compounds which appeared in the reaction mixture were tried by two-dimensional paper chromatography and bioauto graphy and also the recovery of the standard compounds added to the reaction mixture. Then, the isolation and determination of 2•L-deoxyinosine-8-C14 were tested similarly, and the metabolic fate of inosine-8-C14 was examined.