Isotope Effects on the Metabolic &lt;i&gt;N&lt;/i&gt;-Demethylation of Diuron and Monuron in Hepatocytes and Liver Microsomes of Rats

Kurihara, Norio; Kinoshita, K.; Fujikawa, Masato

doi:10.1584/jpestics.15.579

Cited by 1 publication

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“…Cryopreserved rat hepatocytes maintained nearly all of their N-demethylation activity for up to 23 days of freezing -but NADPH addition was required to restore full activity (diuron as substrate) (ref. 21). In contrast when aminopyrine was used as substrate Ndemethylation activity was retained after two days of storage but decreased to 10% after 21 days of storage (ref.…”

Section: Immobilization and Cryopreservation Of Cellsmentioning

confidence: 99%

Pesticides Report 30: Use of Isolated Cells to Study the Metabolism of Agrochemicals in Animals

Kurihara¹,

Paulson²,

Otto³

et al. 2016

IUPAC Standards Online

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Names of countries given after Members' names are in accordance with the ZUPAC Handbook 1991-93; changes will be effected in the 1993-95 edition.Republication of this report is permitted without the need for formal IUPAC permission on condition that an acknowledgement, with full reference together with IUPAC copyright symbol (0 1993 IUPAC), is printed.Publication of a translation into another language is subject to the additional condition of prior approval from the relevant IUPAC National Adhering Organization.Abstract -Isolated cells derived from various organs of animals can be used to obtain a variety of information on the metabolism of agrochemicals and xenobiotics in intact animals. Isolated cells catalyze various phase I and I1 reactions and generally more closely mimic intact animal systems than do other in vitro systems. However the utility of isolated cells is limited by technical difficulties including isolation techniques, preincubation times, separation of heterogenous cells and the difficulty in maintaining catalytic activity for a long period of time. Thus, improved techniques for isolating cells and for maintaining the viability of isolated cells are needed. Studies on the relationship between results obtained with isolated cells and with intact animals, and results obtained with animal cells and human cells are also required. Studies with cells containing expressed human enzymes are desirable.

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Section: Immobilization and Cryopreservation Of Cellsmentioning

confidence: 99%