Isotope-Dilution Mass Spectrometry in Clinical Chemistry

Leenheer, A.P. De; Lefevere, M.F.; Lambert, Willy E.; Colinet, E

doi:10.1016/s0065-2423(08)60272-3

Cited by 61 publications

(22 citation statements)

References 128 publications

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“…Additionally, this approach provides full traceability to the International System of units (SI). IDMS has been well established elsewhere as providing highly reliable quantitative trace analysis [22,23]; thus, it has become the method of choice for the quantification of analytes in primary standards for many national measurement institutes.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of droplet digital PCR for characterizing plasmid reference material used for quantifying ammonia oxidizers and denitrifiers

Meng

Wang

et al. 2014

Anal Bioanal Chem

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DNA reference materials of certified value have a critical function in many analytical processes of DNA measurement. Quantification of amoA genes in ammonia oxidizing bacteria (AOB) and archaea (AOA), and of nirS and nosZ genes in the denitrifiers is very important for determining their distribution and abundance in the natural environment. A plasmid reference material containing nirS, nosZ, amoA-AOB, and amoA-AOA is developed to provide a DNA standard with copy number concentration for ensuring comparability and reliability of quantification of these genes. Droplet digital PCR (ddPCR) was evaluated for characterization of the plasmid reference material. The result revealed that restriction endonuclease digestion of plasmids can improve amplification efficiency and minimize the measurement bias of ddPCR. Compared with the conformation of the plasmid, the size of the DNA fragment containing the target sequence and the location of the restriction site relative to the target sequence are not significant factors affecting plasmid quantification by ddPCR. Liquid chromatography-isotope dilution mass spectrometry (LC-IDMS) was used to provide independent data for quantifying the plasmid reference material. The copy number concentration of the digested plasmid determined by ddPCR agreed well with that determined by LC-IDMS, improving both the accuracy and reliability of the plasmid reference material. The reference value, with its expanded uncertainty (k=2), of the plasmid reference material was determined to be (5.19±0.41)×10 9 copies μL −1 by averaging the results of two independent measurements. Consideration of the factors revealed in this study can improve the reliability and accuracy of ddPCR; thus, this method has the potential to accurately quantify DNA reference materials.

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Section: Introductionmentioning

confidence: 99%

Evaluation of droplet digital PCR for characterizing plasmid reference material used for quantifying ammonia oxidizers and denitrifiers

Meng

Wang

et al. 2014

Anal Bioanal Chem

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“…Then the concentrations of endogenous steroids in each real sample can be calculated based on the regression equations of these calibration lines. Unlike isotope dilution technique that exploits the use of stable isotope-labeled internal standards, in surrogate analyte approach the stable isotope-labeled analyte is not added to every calibration and test sample, but instead is added and quantified only in the calibration samples [36,37]. There are few reports about application of surrogate analyte in chemical analysis [1,3,8,34,35].…”

Section: Resultsmentioning

confidence: 99%

Quantification of endogenous steroids in human urine by gas chromatography mass spectrometry using a surrogate analyte approach

Ahmadkhaniha

Shafiee

Rastkari

et al. 2010

Journal of Chromatography B

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“…Recent reviews on this subject were given by de Leenheer et al (33) and Siekmann (34). Particular problems arise for reference methods for the determination of the catalytic activity concentration of enzyme (35).…”

Section: Analytical Principles For Reference Methodsmentioning

confidence: 99%

Reference Methods as a Basis for Accurate Measuring Systems

Büttner

1991

Clinical Chemistry and Laboratory Medicine

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Summary:Reference methods, äs part of a reference System, are essential for improving the accuracy of routine methods. They can help to establish worldwide compatibility of clinical chemical measurements and they provide an objective basis for quality control measures. The subject of analytical errors is treated in detail, then the concept of reference methods is developed. This review emphasizes the fundamental metrological aspects of reference methods and reference measurement technology. An overview is given of the most important analytical principles used for reference methods in clinical chemistry. The particular problems of reference methods for the determination of the catalytic activity concentration of enzymes are outside the scope of this review.

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Isotope-Dilution Mass Spectrometry in Clinical Chemistry

Cited by 61 publications

References 128 publications

Evaluation of droplet digital PCR for characterizing plasmid reference material used for quantifying ammonia oxidizers and denitrifiers

Evaluation of droplet digital PCR for characterizing plasmid reference material used for quantifying ammonia oxidizers and denitrifiers

Quantification of endogenous steroids in human urine by gas chromatography mass spectrometry using a surrogate analyte approach

Reference Methods as a Basis for Accurate Measuring Systems

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