Isoprene production in Synechocystis under alkaline and saline growth conditions

Chaves, Julie E.; Kirst, Henning; Melis, Anastasios

doi:10.1007/s10811-014-0395-2

Cited by 27 publications

(35 citation statements)

References 28 publications

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“…Therefore, only when the infiltration water moves in the fine capillary can the salt and alkali move down at higher efficiency. According to the capillary management theory of soil moisture and solute transport, when the dripper discharge is large, that is, when the water supply intensity is large, the water moves in the soil capillary under the action of gravity, and the water in the small capillary is relatively immobile, at this time the salt and alkali in the small capillary cannot be leached [30]. Therefore, the efficiency of leaching water is relatively low.…”

Section: Saline-alkali Infiltration Of Drip Irrigation Under Sub-soilmentioning

confidence: 99%

Saline-alkali migration in soda saline soil based on sub-soiling technology

Yuan¹,

Feng²,

Jang³

et al. 2019

Desalination and Water Treatment

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To study the saline-alkali migration law of soda saline-alkali soils based on sub-soiling technology, improve the soil quality of soda saline-alkali agricultural area, and provide scientific basis for the construction of high and stable yield fields of crops in soda saline-alkali soils. Based on the unsaturated soil water movement theory and the convection-dispersion theory of solute transport in porous media, a single-parameter and two-parameter model of saline-alkali transport was constructed to analyze the distribution of saline-alkali concentration in soil solution with the depth of soil layer. Soil samples were taken from four soda saline-alkali soils in different periods under sub-soiling tillage. The experimental data of soil saline-alkali content were obtained, and the law of saline-alkali migration was analyzed. When the depth was 60 cm, the content of saline-alkali in the soil before breaking the plough bottom was 0.4%, and the content of saline-alkali was 0.35% after breaking the plough bottom with sub-soiling technique. It can be seen that sub-soiling technique can promote saline-alkali infiltration and reduce the content of saline-alkali in the planting soil layer. The average water content of the sub-soiled soil was 23.4%, which was significantly higher than that of the subsoiled soil. The soil salinity and alkali content in 100 cm depth layer decreased by 30.8%, and that in 40 cm depth layer decreased by 57%. The salinity and alkali data in different regions showed that the soil salinity and alkali content in the soil decreased by 30.8%. Saline-alkali data from different regions show that due to sub-soiling, salt-alkali in the soil moves upward, salt-alkali in the lower part of crop roots moves outward, and salt-alkali in the soil moves away from the crop root zone; between 0 and 50 cm under the soil, the content of soil salt and alkali is from small to large, forming a desalination area of saline and alkali content; sub-soiling tillage makes 0-30 cm layer soil desalinization rate reach 83.5%, the soil salt removal rate of 0-60 cm layer reached 79.9%; The effect of sub-soiling on soil salinity is less than 1.634% of the initial value. Conclusion: sub-soiling technology can effectively reduce saline alkali content and increase crop yield in soda saline alkali soil.

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Section: Saline-alkali Infiltration Of Drip Irrigation Under Sub-soilmentioning

confidence: 99%

Saline-alkali migration in soda saline soil based on sub-soiling technology

Yuan¹,

Feng²,

Jang³

et al. 2019

Desalination and Water Treatment

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“…Due to their very slow K cat , relatively high K m , and poor expression levels in cyanobacteria, the IspS itself is a limiting factor toward photosynthetic Isp production. PCC 6803 so far [19,29,39,40,62,89,104]. PCC 7942 including: P. montana, Populus trichocarpa, Populus deltoides, Populus alba, Populus canescens, and Eucalyptus globulus [30].…”

Section: Overcoming Limitations In Substrate Flux and Enzyme Concentrationmentioning

confidence: 99%

“…Isp generation has been shown in the fresh water strain Synechocystis sp. PCC 6803 grown in seawater concentrations of 3.5% w : v NaCl [40,62] up to pH 10 [62], showing the natural plasticity of the strain to tolerate a range of environmental conditions, while generating the product of interest. Recently, a S. elongatus sp PCC 7942 strain was developed to tolerate higher stress conditions for outdoor cultivation by overexpression of the hspA gene [63].…”

Section: Barriers To High Capacity Productionmentioning

confidence: 99%

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Engineering isoprene synthesis in cyanobacteria

Chaves

Melis

2018

FEBS Letters

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The renewable production of isoprene (Isp) hydrocarbons, to serve as fuel and synthetic chemistry feedstock, has attracted interest in the field recently. Isp (C H ) is naturally produced from sunlight, CO and H O photosynthetically in terrestrial plant chloroplasts via the terpenoid biosynthetic pathway and emitted in the atmosphere as a response to heat stress. Efforts to institute a high capacity continuous and renewable process have included heterologous expression of the Isp synthesis pathway in photosynthetic microorganisms. This review examines the premise and promise emanating from this relatively new research effort. Also examined are the metabolic engineering approaches applied in the quest of renewable Isp hydrocarbons production, the progress achieved so far, and barriers encountered along the way.

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“…Manuscript to be reviewed productivity is sensitive to most of the aforementioned operational parameters (Yu et al, 2013;Burrows & Wong, 2009;Nanjo et al, 2010;Chaves, Kirst & Melis, 2015), it is undoubted that productivity is tightly coupled with the light absorption efficiency of optical energy conversion systems. Therefore, light management during Synechocystis cultivation in photobioreactors (PBRs) is by far the most remarkable factor to account for in order to boost the practical exploitation of this microorganism.…”

Section: Introductionmentioning

confidence: 99%

Peer Review #1 of "Analysis of the light intensity dependence of the growth of Synechocystis and of the light distribution in a photobioreactor energized by 635 nm light (v0.1)"

2018

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Synechocystis gathered momentum in modelling studies and biotechnological applications owing to multiple factors like fast growth, ability to fix carbon dioxide into valuable products, and the relative ease of genetic manipulation. Synechocystis physiology and metabolism, and consequently, the productivity of Synechocystis-based photobioreactors, are heavily light modulated. Here, we set up a turbidostat-controlled lab-scale cultivation system in order to study the influence of varying orange-red light intensities on Synechocystis growth characteristics and photosynthetic activity. Synechocystis growth and photosynthetic activity were found to raise as supplied light intensity increased up to 500 µmol photons m -2 s -1 and to enter the photoinhibition state only at 800 µmol photons Manuscript to be reviewed 47In the latter case, the heterogeneous distribution of the local light intensity would allow 48Synechocystis for an optimized usage of light.

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Isoprene production in Synechocystis under alkaline and saline growth conditions

Cited by 27 publications

References 28 publications

Saline-alkali migration in soda saline soil based on sub-soiling technology

Saline-alkali migration in soda saline soil based on sub-soiling technology

Engineering isoprene synthesis in cyanobacteria

Peer Review #1 of "Analysis of the light intensity dependence of the growth of Synechocystis and of the light distribution in a photobioreactor energized by 635 nm light (v0.1)"

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