Isoprene: New insights into the control of emission and mediation of stress tolerance by gene expression

Lantz, Alexandra T.; Allman, J. Dowling; Weraduwage, Sarathi M.; Sharkey, Thomas D.

doi:10.1111/pce.13629

Cited by 62 publications

(47 citation statements)

References 196 publications

(303 reference statements)

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“…The roles of isoprene in abiotic stress responses in plants have been discussed by Vickers et al [9,10]. Recent studies have demonstrated that isoprene influences gene expression in plants, as reviewed by Lantz et al [12]. For example, Arabidopsis thaliana, a non-isoprene emitter, engineered to express ispS from Eucalyptus globulus, demonstrated regulation of isoprene emission and photosynthesis pathways which were comparable to the native emitter [11].…”

Section: Introductionmentioning

confidence: 99%

Isoprene Oxidation by the Gram-Negative Model bacterium Variovorax sp. WS11

et al. 2020

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Plant-produced isoprene (2-methyl-1,3-butadiene) represents a significant portion of global volatile organic compound production, equaled only by methane. A metabolic pathway for the degradation of isoprene was first described for the Gram-positive bacterium Rhodococcus sp. AD45, and an alternative model organism has yet to be characterised. Here, we report the characterisation of a novel Gram-negative isoprene-degrading bacterium, Variovorax sp. WS11. Isoprene metabolism in this bacterium involves a plasmid-encoded iso metabolic gene cluster which differs from that found in Rhodococcus sp. AD45 in terms of organisation and regulation. Expression of iso metabolic genes is significantly upregulated by both isoprene and epoxyisoprene. The enzyme responsible for the initial oxidation of isoprene, isoprene monooxygenase, oxidises a wide range of alkene substrates in a manner which is strongly influenced by the presence of alkyl side-chains and differs from other well-characterised soluble diiron monooxygenases according to its response to alkyne inhibitors. This study presents Variovorax sp. WS11 as both a comparative and contrasting model organism for the study of isoprene metabolism in bacteria, aiding our understanding of the conservation of this biochemical pathway across diverse ecological niches.

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Section: Introductionmentioning

confidence: 99%

Isoprene Oxidation by the Gram-Negative Model bacterium Variovorax sp. WS11

et al. 2020

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“…The biological role for isoprene in plants is a continuing matter for debate and has been reviewed recently by Lantz et al . [14] and Sharkey and Monson [15]. Since trees are the major source of isoprene globally [7], there is increasing interest in the potential impact on air quality of certain crop plants [16].…”

Section: Isoprene Production In the Biosphere And Effects On Climatementioning

confidence: 99%

Microbial metabolism of isoprene: a much-neglected climate-active gas

2020

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The climate-active gas isoprene is the major volatile produced by a variety of trees and is released into the atmosphere in enormous quantities, on a par with global emissions of methane. While isoprene production in plants and its effect on atmospheric chemistry have received considerable attention, research into the biological isoprene sink has been neglected until recently. Here, we review current knowledge on the sources and sinks of isoprene and outline its environmental effects. Focusing on degradation by microbes, many of which are able to use isoprene as the sole source of carbon and energy, we review recent studies characterizing novel isoprene degraders isolated from soils, marine sediments and in association with plants. We describe the development and use of molecular methods to identify, quantify and genetically characterize isoprene-degrading strains in environmental samples. Finally, this review identifies research imperatives for the further study of the environmental impact, ecology, regulation and biochemistry of this interesting group of microbes.

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“…However, which terpene should be better suited to this task is still a matter of debate, as the evidence in favor of either of them is still fragmentary and partly conflicting. The presence of isoprene emission in wild soybean (Glycine soja) and its lack in cultivated soybean (Glycine max) suggests that isoprene emission in Fabaceae could have been counter-selected during the domestication process in favor of monoterpene emission [46]. Given the naturally occurring multiple losses and gains of isoprene emission during the course of evolution in Fabaceae [12], however, it is still doubtful whether IspS pseudogenization in cultivated soybean is simply a by-product or the result of domestication analogously to the loss of resistance toward pathogens observed in several other crops [47,48].…”

Section: Agricultural and Evolutionary Relevancementioning

confidence: 99%

Shoot Characterization of Isoprene and Ocimene-Emitting Transgenic Arabidopsis Plants under Contrasting Environmental Conditions

Faralli

Varotto

2020

Plants

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Isoprenoids are among the most abundant biogenic volatile compounds (VOCs) emitted by plants, and mediate both biotic and abiotic stress responses. Here, we provide for the first time a comparative analysis of transgenic Arabidopsis lines constitutively emitting isoprene and ocimene. Transgenic lines and Columbia-0 (Col-0) Arabidopsis were characterized under optimal, water stress, and heat stress conditions. Under optimal conditions, the projected leaf area (PLA), relative growth rate, and final dry weight were generally higher in transgenics than Col-0. These traits were associated to a larger photosynthetic capacity and CO 2 assimilation rate at saturating light. Isoprene and ocimene emitters displayed a moderately higher stress tolerance than Col-0, showing higher PLA and gas-exchange traits throughout the experiments. Contrasting behaviors were recorded for the two overexpressors under water stress, with isoprene emitters showing earlier stomatal closure (conservative behavior) than ocimene emitters (non-conservative behavior), which might suggest different induced strategies for water conservation and stress adaptation. Our work indicates that (i) isoprene and ocimene emitters resulted in enhanced PLA and biomass under optimal and control conditions and that (ii) a moderate stress tolerance is induced when isoprene and ocimene are constitutively emitted in Arabidopsis, thus providing evidence of their role as a potential preferable trait for crop improvement.

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Isoprene: New insights into the control of emission and mediation of stress tolerance by gene expression

Cited by 62 publications

References 196 publications

Isoprene Oxidation by the Gram-Negative Model bacterium Variovorax sp. WS11

Isoprene Oxidation by the Gram-Negative Model bacterium Variovorax sp. WS11

Microbial metabolism of isoprene: a much-neglected climate-active gas

Shoot Characterization of Isoprene and Ocimene-Emitting Transgenic Arabidopsis Plants under Contrasting Environmental Conditions

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