Isoplanatic patch of the human eye for arbitrary wavelengths

Han, Ge; Cao, Zhaoliang; Mu, Quanquan; Wang, Yukun; Li, Dayu; Wang, Shaoxin; Xu, Zihao; Wu, Daosheng; Hu, Liang; Li, Xuan

doi:10.1016/j.optcom.2017.11.055

Cited by 6 publications

(5 citation statements)

References 15 publications

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“…On the other, this separation can actually be large enough to cause resolution degradation due to anisoplanatism between the two regions of interest. 13,14,15 The isoplanatic patch size can loosely be considered the maximal size of a field of view for which any two points can be reasonably considered to have the same optical aberrations. This size varies tremendously with the object in question, and the pupil size being used, but for a dilated primate eye, it is on the order of 1 degree of visual angle.…”

Section: Design Considerationsmentioning

confidence: 99%

A dual adaptive optics instrument for testing the role of retinal ganglion cells in vision

Teverovsky,

Murphy,

Parkins

et al. 2024

Ophthalmic Technologies XXXIV

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Retinal ganglion cells (RGCs) are the layer of neurons in the retina that output directly to the brain. In primates, there are approximately 20 distinct types which each transmit an independent encoding of the external visual world in parallel to the brain. 1 The wide variety of RGC types suggests that the retina undertakes a sophisticated set of computations that our used by higher brain areas to decode the visual world. Yet, little is known about the majority of the different types of RGCs present in the primate retina largely due to the rarity of many of them. Current understanding of RGCs largely derives from ex vivo electrophysiology experiments which are acute and require severing the optic nerve. 2 This preparation removes the opportunity to study vision as an intact system and puts a time limit on how long a sample is viable for study before the tissue dies. Traditional electrophysiology also struggles to study the RGCs that serve the fragile fovea, the central high-resolution region of the retina. Adaptive optics (AO) ophthalmoscopy coupled with functional imaging and optogenetics provides a unique set of tools which allows the study of individual RGCs in situ. 3,4 Here we describe a plan for a novel split field of view AO ophthalmoscope that will deliver visual stimuli precisely controlled in space, time, and color to the receptive fields of specific RGCs, while also simultaneously optically recording functional calcium responses from the same RGCs. The device also enables psychophysical experiments to study the perceptual impact of RGC types via optogenetics.

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Section: Design Considerationsmentioning

confidence: 99%

A dual adaptive optics instrument for testing the role of retinal ganglion cells in vision

Teverovsky,

Murphy,

Parkins

et al. 2024

Ophthalmic Technologies XXXIV

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“…3, this algorithm could also be modified to account for the correction of nonisoplanatic aberrations. 47,48 Our aim is to obtain bright and small spots on a low zero-order background after the application of the aberrations-correction algorithm.…”

Section: Aberrations Representational Modelmentioning

confidence: 99%

“…Moreover, we expect that living tissues display anisoplanatic aberrations. 47,48,55 We have tested the optical setup for possible anisoplanatism by performing corrections on arrays on adjacent ROIs covering a wide field of view (see Fig. 3).…”

Section: Aberrations Correctionmentioning

confidence: 99%

Adaptive optics microspectrometer for cross-correlation measurement of microfluidic flows

et al. 2019

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Mapping flows in vivo is essential for the investigation of cardiovascular pathologies in animal models.The limitation of optical-based methods, such as space-time cross correlation, is the scattering of light by the connective and fat components and the direct wave front distortion by large inhomogeneities in the tissue. Nonlinear excitation of the sample fluorescence helps us by reducing light scattering in excitation. However, there is still a limitation on the signal-background due to the wave front distortion. We develop a diffractive optical microscope based on a single spatial light modulator (SLM) with no movable parts. We combine the correction of wave front distortions to the cross-correlation analysis of the flow dynamics. We use the SLM to shine arbitrary patterns of spots on the sample, to correct their optical aberrations, to shift the aberration corrected spot array on the sample for the collection of fluorescence images, and to measure flow velocities from the cross-correlation functions computed between couples of spots. The setup and the algorithms are tested on various microfluidic devices. By applying the adaptive optics correction algorithm, it is possible to increase up to 5 times the signalto-background ratio and to reduce approximately of the same ratio the uncertainty of the flow speed measurement. By working on grids of spots, we can correct different aberrations in different portions of the field of view, a feature that allows for anisoplanatic aberrations correction. Finally, being more efficient in the excitation, we increase the accuracy of the speed measurement by employing a larger number of spots in the grid despite the fact that the two-photon excitation efficiency scales as the fourth power of this number: we achieve a twofold decrease of the uncertainty and a threefold increase of the accuracy in the evaluation of the flow speed. © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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“…11,12 Current AOOs have small fields of view (FOV), typically ±1.5 • , which is set by the largest area that can be imaged while maintaining diffraction limited resolution through a fully dilated ocular pupil. 13,14 This diffraction limited area is called the isoplanatic patch. The size of the isoplanatic patch depends on many factors, but it critically has an inverse relationship with ocular pupil size.…”

Section: Introductionmentioning

confidence: 99%

First order solution generation for reflective dual-conjugate zoom systems

Teverovsky,

Smith,

Bentley

2024

Optical Design and Engineering IX

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Adaptive optics (AO) ophthalmoscopy is a powerful technique for imaging retinas of living subjects at single-cell resolution. Traditional AO ophthalmoscopes use a fixed exit pupil diameter which is matched to the ocular pupil of a subject. A more efficient ophthalmoscope would allow for a variable exit pupil size to match the variable ocular pupil size of a subject. One method to achieve this is an optical zoom system. To make a zoom system appropriate for use in adaptive optics ophthalmoscopy it must be simultaneously well-corrected for both the field conjugate and the pupil conjugate such that the aberration correction can be successfully applied. The system must also be made of reflective optics to minimize chromatic aberration and spurious back reflections propagating through the system. Both these factors make for an extremely novel zoom system that requires new tools and methods to be developed in order to realize successful designs.We present here our work developing a new first-order starting point generator which creates valid first-order solutions that satisfy all the constraints of the system. We then show our techniques for modeling and optimizing the simultaneous conjugates of the system throughout the process of unobscuring the system. We find that to achieve the selected specifications that freeform optics must be introduced to the system to correct residual errors created during the unobscuring process.

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Isoplanatic patch of the human eye for arbitrary wavelengths

Cited by 6 publications

References 15 publications

A dual adaptive optics instrument for testing the role of retinal ganglion cells in vision

A dual adaptive optics instrument for testing the role of retinal ganglion cells in vision

Adaptive optics microspectrometer for cross-correlation measurement of microfluidic flows

First order solution generation for reflective dual-conjugate zoom systems

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