Isopimpinellin extends antiangiogenic effect through overexpression of miR-15b-5p and downregulating angiogenic stimulators

Bhagavatheeswaran, Sambhavi; Ramachandran, Vinu; Shanmugam, Sambantham; Balakrishnan, Anandan

doi:10.1007/s11033-021-06870-4

Cited by 7 publications

(6 citation statements)

References 47 publications

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“…The (Paramasivam et al, 2012). Another phytochemical, isopimpinellin, was shown to have an anti-angiogenic effect by significantly inhibiting ISV in a dose-dependent pattern (Bhagavatheeswaran et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

“…Similar results were observed in an earlier study with thymoquinone, a flavonoid derived from black cumin Nigella sativa seeds, that inhibits angiogenesis by lowering ISV development in treated zebrafish embryos (Paramasivam et al, 2012). Another phytochemical, isopimpinellin, was shown to have an anti‐angiogenic effect by significantly inhibiting ISV in a dose‐dependent pattern (Bhagavatheeswaran et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

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Role of berberine on angiogenesis and blood flow hemodynamics using zebrafish model

Nathan,

Shameera,

Devarajan

et al. 2023

J of Applied Toxicology

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Angiogenesis and hemodynamic instability created by the irregular blood vessels causes hypoperfusion and angiogenesis‐mediated diseases. Therefore, therapies focusing on controlling angiogenesis will be a valuable approach to treat a broad spectrum of diseases. In this study, we explored the anti‐angiogenic potential of berberine (BBR) and also analyzed blood flow hemodynamics using zebrafish embryos. Zebrafish embryos treated with BBR (0.01–0.75 mM) at various doses at 1 hour post‐fertilization (hpf) developed a variety of phenotypic variations including aberrant blood vessels, tail bending, edema, and hemorrhage. Survival rates were much lower at higher dosages, and hatching rates were almost 99%, whereas control group appeared normal. Heart rate is an essential measure that has a strong association with hemodynamics. We used ImageJ software to study the heart rate of embryos treated with BBR, preceded by video processing. The resultant graph shows a significant decrease in heart rate of embryos treated with BBR in dose‐dependent manner. Also, RBC staining using o‐Dianisidine confirms the anti‐angiogenic potential of BBR by indicating the decrease in the intersegmental vessels at 0.5 and 0.75 mM treated embryos. Further, the gene expression study determined that the transcripts (vegf, vegfr2, nrp1a, hif‐1α, nos2a, nos2b, cox‐2a, and cox‐2b) measured were found to be downregulated by BBR at 0.5 mM concentration, from which we conclude that enos/vegf signaling could play an important role in modulating angiogenesis. Our data imply that BBR may be an effective compound for suppressing angiogenesis in vivo, which might be helpful in the treatment of vascular disorders like cancer and diabetic retinopathy in future.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Role of berberine on angiogenesis and blood flow hemodynamics using zebrafish model

Nathan,

Shameera,

Devarajan

et al. 2023

J of Applied Toxicology

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“…miR-15b-5p has also been shown to mediate the anti-amyloid effect of curcumin in an in vitro model of Alzheimer’s disease through influencing expression of the amyloid precursor protein ( 36 ). Moreover, the antiangiogenic effect of isopimpinellin has been attributed to its impact on induction of miR-15b-5p expression and subsequent down-regulation of angiogenic stimulators ( 37 ).…”

Section: Role Of Mir-15b-5p In Non-malignant Conditionsmentioning

confidence: 99%

A Comprehensive Review on Function of miR-15b-5p in Malignant and Non-Malignant Disorders

et al. 2022

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miR-15b-5p is encoded by MIR15B gene. This gene is located on cytogenetic band 3q25.33. This miRNA participates in the pathogenesis of several cancers as well as non-malignant conditions, such as abdominal aortic aneurysm, Alzheimer’s and Parkinson’s diseases, cerebral ischemia reperfusion injury, coronary artery disease, dexamethasone induced steatosis, diabetic complications and doxorubicin-induced cardiotoxicity. In malignant conditions, both oncogenic and tumor suppressor impacts have been described for miR-15b-5p. Dysregulation of miR-15b-5p in clinical samples has been associated with poor outcome in different kinds of cancers. In this review, we discuss the role of miR-15b-5p in malignant and non-malignant conditions.

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“…Compound 18 yielded the deprotonated molecular ions at m/z 247.0598 and characteristic fragment ions of m/z 232.0362 [M+H‐CH 3 ] + , 217.0128 [M+H‐2CH 3 ] + and 189.0180 [M+H‐2CH 3 ‐CO] + . Thus, compound 18 was deduced to be isopimpinellin [27], which possesses anti‐angiogenic and anti‐cancer effects [28]. Its detailed fragmentation pathway is exhibited in Figure 3D.…”

Section: Resultsmentioning

confidence: 99%

Analysis of the chemical components of pomelo peels (Citrus grandis [L.] Osbeck) from different cultivars by using supercritical CO₂ fluid extraction and ultra‐high‐performance liquid chromatography‐tandem mass spectrometry

Tian

Liu

et al. 2022

J of Separation Science

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Five pomelo cultivars (i.e., Citrus grandis cv. Shatianyou, Citrus grandis cv. Guanximiyou, Citrus grandis cv. Yuhuanyou, Citrus grandis cv. Duweiwendanyou and Citrus grandis cv. Liangpingyou) from different origins in China were selected to analyze their components by using supercritical CO2 fluid extraction coupled with ultra‐high‐performance liquid chromatography‐tandem mass spectrometry. A total of 45 compounds were identified in the supercritical CO2 fluid extracts of the pomelo peels from the five cultivars. These compounds included eight flavonoids, 18 coumarins, four organic acids, three aldehydes, and 12 other compounds, which were identified using the obtained MS data and by comparison with commercial standards, orbitrap Chinese Traditional Medicine Library, and previous literature. Twenty‐five of the identified compounds were detected for the first time in the pomelo peel extracts. Results suggested that the pomelo peels of C. grandis cv. Shatianyou contained the most natural chemical compositions. The pooled result may offer scientific evidence for further development and utilization of pomelo peels and a route for screening appropriate varieties for various demands.

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Isopimpinellin extends antiangiogenic effect through overexpression of miR-15b-5p and downregulating angiogenic stimulators

Cited by 7 publications

References 47 publications

Role of berberine on angiogenesis and blood flow hemodynamics using zebrafish model

Role of berberine on angiogenesis and blood flow hemodynamics using zebrafish model

A Comprehensive Review on Function of miR-15b-5p in Malignant and Non-Malignant Disorders

Analysis of the chemical components of pomelo peels (Citrus grandis [L.] Osbeck) from different cultivars by using supercritical CO₂ fluid extraction and ultra‐high‐performance liquid chromatography‐tandem mass spectrometry

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Isopimpinellin extends antiangiogenic effect through overexpression of miR-15b-5p and downregulating angiogenic stimulators

Cited by 7 publications

References 47 publications

Role of berberine on angiogenesis and blood flow hemodynamics using zebrafish model

Role of berberine on angiogenesis and blood flow hemodynamics using zebrafish model

A Comprehensive Review on Function of miR-15b-5p in Malignant and Non-Malignant Disorders

Analysis of the chemical components of pomelo peels (Citrus grandis [L.] Osbeck) from different cultivars by using supercritical CO2 fluid extraction and ultra‐high‐performance liquid chromatography‐tandem mass spectrometry

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Product

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Analysis of the chemical components of pomelo peels (Citrus grandis [L.] Osbeck) from different cultivars by using supercritical CO₂ fluid extraction and ultra‐high‐performance liquid chromatography‐tandem mass spectrometry