Isoliquiritigenin Attenuates Adipose Tissue Inflammation and Metabolic Syndrome by Modifying Gut Bacteria Composition in Mice

Abstract

Scope: Isoliquiritigenin (ILG) has been reported to attenuate adipose tissue inflammation and metabolic disorder; however, the underlying mechanisms remain to be elucidated. The aim of this study is to elucidate whether ILG shows the anti-inflammatory and antimetabolic syndrome effects through gut microbiota modification. Methods and results: Mice are fed a high-fat diet (HFD) with or without ILG for up to 12 weeks. The effect of ILG on body weight, blood glucose level, adipose tissue inflammation, gut barrier function, and gut microbiota composition are investigated. ILG supplementation alleviates HFD-induced obesity, glucose tolerance, and insulin resistance and suppresses inflammatory gene expression in epididymal white adipose tissue (eWAT). Moreover, ILG supplementation modifies gut bacterial composition by increasing the abundance of antimetabolic disease-associated species (e.g., Parabacteroides goldsteinii and Akkemansia muciniphila) and up-regulated genes associated with gut barrier function. Fecal microbiome transplantation (FMT) from ILG-fed donors counteract HFD-induced body and eWAT weight changes, inflammation-related gene expression, glucose tolerance, and insulin resistance, thereby suggesting that ILG-responsive gut bacteria exerts anti-inflammatory and antimetabolic syndrome effects. Conclusion: Alterations in gut bacteria underly the beneficial effects of ILG against adipose tissue inflammation and metabolic disorders. ILG may be a promising prebiotic for the prevention and treatment of metabolic syndrome.

Impact of Vancomycin Treatment and Gut Microbiota on Bile Acid Metabolism and the Development of Non-Alcoholic Steatohepatitis in Mice

“…For sequencing, 16S rRNA gene sequence libraries were prepared according to the Illumina (San Diego, CA, USA) protocol, as described previously [ 48 ]. The final libraries were pooled, diluted to 4 nM in 5 mM Tris-HCl buffer, and sequenced using a Miseq System with a 600-Cycle Kit (Illumina).…”

“…The Quantitative Insight Into Microbial Ecology 1 (QIIME1) pipeline, an open-source bioinformatics tool for the analysis of raw microbiome DNA sequencing data, was used to investigate the bacterial composition as described previously [ 48 ]. The taxonomy assignment was performed using the EzbioCloud reference database.…”

Effect of soluble oat fiber on intestinal microenvironment and TNBS-induced colitis

“…Cecal and fecal SCFAs were measured using GC as previously described. 18 For extraction of cecal and fecal SCFAs, cecal contents and feces were placed in 10 volumes of water and intensively homogenized using a toothpick until fully suspended. The supernatants were transferred to new tubes and heptanoic acid was added as an internal standard.…”

Fecal microbiota transplantation modulates the intestinal microbiome and alleviates Escherichia coli infected outcomes in mice

“…For FMT, 100 mg of fresh fecal pellets were collected daily from donor mice immediately after defecation, suspended in 1 mL normal saline, vortexed for 1 min, and centrifuged at 2500 × g for 1 min. Then 100 μL aliquots of supernatant from the stool samples were administered to recipient mice by gavage immediately to minimize the alterations in the composition of fecal microbiome [19].…”