Isolierung von freien Nucleotiden aus verschiedenen Geweben

Schmitz, Hanns; Potter, R. Van; Hurlbert, Robert B.

doi:10.1007/bf00630992

Cited by 15 publications

(6 citation statements)

References 15 publications

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“…Gumaa & McLean (1969) have estimated that 80% of pentose 5-phosphate was formed non-oxidatively by Krebs ascites cells. Other studies by Kit et al (1957) using lymphatic cells and tumours, Hiatt (1957) using HeLa cells, Schneider & Potter (1957) with regenerating liver, Schmitz et al (1954) using Flexner-Jobling carcinoma and rat liver indicated that a significant proportion of the ribose 5-phosphate moiety of nucleic acid was formed nonoxidatively.…”

Section: Reversibility Of the Non-oxidative Segment Of The Pentose Cyclementioning

confidence: 95%

Use of [2-14C]glucose and [5-14C]glucose for evaluating the mechanism and quantitative significance of the ‘liver-cell’ pentose cycle

Longenecker¹,

Williams²

1980

Biochemical Journal

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1. Expressions are derived for the steady-state measurement of the quantitative contribution of the liver-type pentose phosphate cycle to glucose metabolism by tissues. One method requires the metabolism of [5-14C]glucose followed by the isolation and degradation of glucose 6-phosphate. The second procedure involves the metabolism of [2-14C]glucose and the isolation and degradation of a triose phosphate derivative, usually lactate or glycerol. 2. Measurements of 14C in C-2 and C-5 of glucose 6-phosphate are required and the values of the C-2/C-5 ratios can be used to calculate the quantitative contribution of the L-type pentose cycle in all tissues. 3. The measurement of 14C in C-1, C-2 and C-3 of triose phosphate derivatives can be used to calculate the quantitative contribution of the L-type pentose cycle relative to glycolysis. 4. The effect of transaldolase and transketolase exchange reactions, reactions of gluconeogenesis and non-oxidative formation of pentose 5-phosphate, isotopic equilibration of triose phosphate pools and isotopic equilibration of fructose 6-phosphate and glucose 6-phosphate, which could interfere with a clear interpretation of the data using [2-14C]- and [5-14C]glucose are discussed.

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Section: Reversibility Of the Non-oxidative Segment Of The Pentose Cyclementioning

confidence: 95%

Use of [2-14C]glucose and [5-14C]glucose for evaluating the mechanism and quantitative significance of the ‘liver-cell’ pentose cycle

Longenecker¹,

Williams²

1980

Biochemical Journal

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“…Under the experimental conditions, guanosine 5'-pyrophosphate could not be detected after bacteria had been grown in the presence of labelled guanine. The presence of guanosine pyrophosphate in the acid-soluble portion of rat tissues has been reported (Schmitz, Hurlbert & Potter, 1954).…”

Section: Discussionmentioning

confidence: 98%

The effects of 8-azaguanine on the biosynthesis of ribonucleic acid in Bacillus cereus

Mandel

Markham

1958

Biochemical Journal

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LEUCOCYTES AND NON-PLASMA ANTIBODY 297 4. This non-plasma antibody is unlikely to be mainly in the lymphocytes or lymphoid tissue. 5. Injection of antigen into immune, but not into normal, rabbits causes a profound leucopenia, which is due to a fall in the numbers of both lymphocytes and granulocytes. This phenomenon is not modified by the prior injection of cortisone in amounts sufficient to cause a lymphocytopenia. We are most grateful to Professor A. Wormall for his constant advice and encouragement. We are indebted to the Medical Research Council and the Central Research Fund of the University of London for grants (to Professor A. Wormall) which have partly covered the costs of these investigations. We are also thankful to Miss A. Routledge for technical help throughout these investigations.

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“…The methods have inairily heen used for liver tissue (HuR1,RERr et al 1954 andBRUMM. POTTER andSieKxvrrz 1956), h e r mitochondria (SIEKEvI'rz slid POTTER 1955, BEYER, GLOMSET, andBEYER 1955), yeast cells (SCHMITZ 1954) and cancer cells ( SCHMITZ, POTTER, HURLRERT and WHITE 1954) hut not for kidney tissue according to the author's knowledge. The metabolism of phosphocompounds in the kidney in hyperglycemia has been studied by DRATZ and HANDLER (1952).…”

Section: Discossioiimentioning

confidence: 96%

The Incorporation of Labelled Phosphate into Different Compounds in the Rat Kidney. The Effect of Parathyroid Extract on an in vivo System.

Verdier

1957

Acta Physiologica Scandinavica

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According to most investigators the renal tubule is the prime locus for the action of the parathyroid hormone (e. g. JAt:om antl VERRANCR 1953, BARTTER 1954. In a recent article DAVIES. GORDON and MUSSETT (1956) have shown that parathyroid extract can be separated into one fraction which acts mainly on the excretion of phosphate by the kidney and another which mainly causes a liberation of bone salts.During recent years there has been a tendency t o localize the action of hormones to special chemical reactions (e. y. SUTHER-LAND 1955). Concerning parathormone IMRIE and HELKINSOS (1932) have observed that it accelerates the synthesis of creatine phosphate in cat's muscle, and quite recently NEUMAN, CHEK. FIRXCHEIN, MULRYAN and DI STEPANO (1956) have presented atheory, that the action of the hormone on the production of citrate in bone may be explained by a destruction of TPK".Abbreviations w e used as follov,s: AMP, ADP, ATP ~ adenosine mono-. di-, and triphosphate; ITMI', UDP uridine mono-, and diphosphate; CIMI'. GIIPguanidine mono-, and diphosphate; CMP ~ idine monophosphate; I M P inosine monophosphate; DPK and TPN : diphoqpho-and tripho.phopyridine nur-lcotides; 1P ~ inorganir orthophosphate; G-1-P and Q-6-P glycose-1-, a n d glucose-&phosphate; Y-6-P = fructose-&phosphate; SerP antl ThrP swine-, and threonine-0-phosphate: TC'A : triclilotoaretic acid. 1-573087. Artn p h g s . Sccindinrru. I'CJI. 49.

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Isolierung von freien Nucleotiden aus verschiedenen Geweben

Cited by 15 publications

References 15 publications

Use of [2-14C]glucose and [5-14C]glucose for evaluating the mechanism and quantitative significance of the ‘liver-cell’ pentose cycle

Use of [2-14C]glucose and [5-14C]glucose for evaluating the mechanism and quantitative significance of the ‘liver-cell’ pentose cycle

The effects of 8-azaguanine on the biosynthesis of ribonucleic acid in Bacillus cereus

The Incorporation of Labelled Phosphate into Different Compounds in the Rat Kidney. The Effect of Parathyroid Extract on an in vivo System.

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