Isolations of subgenus III salmonellas (arizonas) in Cardiff, 1959–1971

SUMMARYOne hundred and eighty samples of pork sausages were examined after preenrichment in buffered peptone water (P medium), for the presence of salmonellas. From each pre-enrichment four enrichments were made: (1) 0-1 ml of P medium was inoculated into 10 ml of Rappaport's medium, formula R 10 (R 10/43°C), (2) 1 ml of the P medium was added to 100 ml of R 10 broth (RIO/100 ml/43°C), (3) 1 ml of P medium was inoculated into 10 ml of Muller-Kauffmann tetrathionate broth (MK medium) prepared in accordance with the International Standards Organization document ISO 3565 (MK/43°C) and (4) 10 ml of P medium were added to 100 ml of MK broth (MK/100 ml/43°C). All the enrichments were incubated at 43°C for 48 h. Forty-six and 47 samples were found positive with the first two enrichment methods (R 10/43°C and R 10/100 ml/43°C), while only 16 samples were found positive with the method MK/43°C, and 27 with the method MK/ 100 ml/43 'C. The superiority ofeither one of the two R 10 procedures over either one of the two MK methods is statistically highly significant (paired x2; P < 0-001 in all four comparisons). The superiority of procedure MK/100 ml/43 'C over the method MK/43 'C is also statistically significant (P < 0005). Both R 10/43°C and R 10/100 ml/43°C procedures had a much stronger inhibitory effect on the competing organisms (lactose-and sucrosenegative) than the two MK methods.

Section: Isolation Of Salmonella Methodsmentioning

confidence: 82%

Isolation of Salmonella with the use of 100 ml of the R10 modification of Rappaport's enrichment medium

Vassiliadis

Trichopoulos

Kalapothaki

et al. 1981

“…The carriage of S. typhi in European gulls (Steiniger, 1970) and of subgenus III salmonellae in Australian silver gulls (Western Australia Public Health Department, 1977) has been reported. Although these serotypes are relatively infrequently experienced in the United Kingdom and to our knowledge have not been isolated from seagulls in Scotland, if these serotypes, or indeed S. dublin were being sought from gulls, an alternative enrichment medium such as selenite or tetrathionate broth should be employed, as Rappaport's medium is not suitable for the isolation of these particular serotypes (Rappaport, Konforti & Navon, 1959;Harvey, Price & Hall, 1973;Harvey & Price, 1975).…”

Section: Resultsmentioning

confidence: 99%

A comparison of enrichment media for the isolation of salmonellae from seagull cloacal swabs

Fricker

Girdwood

Munro

1983

SUMMARYThe relative efficiency of three selective enrichment broths (Muller-Kauffmann tetrathionate, Rappaport's and selenite F) was investigated for the isolation of salmonellae from seagull cloacal swabs. Pre-enrichment in buffered peptone water was employed throughout the study, which involved the examination of 560 gulls, sixty (10-7%) of which were found to be carrying salmonellae. Rappaport's broth as modified by Vassiliadis for incubation at 43 'C (Vassiliadis et al. 1976) yielded the highest number of positive swabs (57) and the widest range of serotypes. It was significantly more efficient that either selenite F or tetrathionate broth, although the results obtained with Rappaport's broth incubated at 37 and 43°C did not differ significantly (P > 0 5). Eleven serotypes were isolated during. the study, the most prevalent being Salmonella virchow.

“…Adjustments of method must, of course, be made if S. typhi, S. dublin or lactose fermenting salmonella are thought to be present in the sample. (Harvey & Price, 1975;Harvey, Price & Hall, 1973).…”

Section: Resultsmentioning

confidence: 99%

Comparison of selenite F, Muller-Kauffmann tetrathionate and Rappaport's medium for salmonella isolation from chicken giblets after pre-enrichment in buffered peptone water

Harvey

Price

1981

Self Cite

SUMMARYSix hundred and eighty three samples of chicken giblets were examined for salmonellas. Three hundred and forty nine of these were neck and crop specimens and 334 were combined liver and heart samples. Two hundred and ten, in all, contained salmonellas.The technique of examination included pre-enrichment in buffered peptone water at 37°C for 18 h and subculture to three enrichment media: MullerKauffmann tetrathionate, selenite F and Rappaport's magnesium chloride malachite green broth. Inocula from buffered peptone water to 10 ml of tetrathionate and selenite were 1 ml in each case. The inoculum from the pre-enrichment medium to 10 ml of Rappaport was 0 005 ml. Tetrathionate and selenite were incubated at 43°C for 48 h. Rappaport's medium was incubated at 37°C for 48 h. Subcultures from all three enrichment broths were made at 24 h and 48 h to brilliant green MacConkey agar. Selective agars were incubated at 37°C for 24 h.The most successful technique for salmonella isolation used Rappaport's medium, which was significantly more efficient than either tetrathionate or selenite. This finding reinforces results obtained using sewage polluted natural water as test material and it is suggested that routine examination of environment samples for salmonellas could be based on Rappaport's medium alone.If S. typhi, S. dublin or subgenus III salmonellas were likely to be present in the sample, the technique described here would require modification.