Isolation, tissue expression, and chromosomal assignment of human RGS5, a novel G-protein signaling regulator gene

Seki, Naohiko; Sugano, Sumio; Suzuki, Yutaka; Nakagawara, Akira; Ohira, Miki; Muramatsu, Masaaki; Saito, Toshiyuki; Hori, Tada-aki

doi:10.1007/s100380050071

Cited by 50 publications

(34 citation statements)

References 17 publications

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“…35 RGS5 has also been identified by microarray analysis of genes expressed in the media of human aorta but not vena cava, suggesting specific arterial expression. 24 In a search for genes differentially expressed in a genetic rat model for stroke, Kirsch and colleagues 25 found RGS5 to be down-regulated, and they localized RGS5 expression in the rat CNS to capillary endothelium and the plexus choroideus.…”

Section: Discussionmentioning

confidence: 97%

Transcription Profiling of Platelet-Derived Growth Factor-B-Deficient Mouse Embryos Identifies RGS5 as a Novel Marker for Pericytes and Vascular Smooth Muscle Cells

Bondjers

Kalén²,

Hellström³

et al. 2003

The American Journal of Pathology

213

163

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Section: Discussionmentioning

confidence: 97%

Transcription Profiling of Platelet-Derived Growth Factor-B-Deficient Mouse Embryos Identifies RGS5 as a Novel Marker for Pericytes and Vascular Smooth Muscle Cells

Bondjers

Kalén²,

Hellström³

et al. 2003

The American Journal of Pathology

213

163

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“…It has been shown to be expressed at high levels in heart, lung, skeletal muscle, and small intestine. 42 Since there are no antibodies available for immunohistochemical staining, we used ISH to evaluate the expression of these two novel endothelial cell markers in HCC and nontumor liver tissues. In this study, we assessed the expression of MSTP032 and RGS5 (two cluster I genes) with CDH5, a cluster II gene.…”

Section: Ish Study Of Novel Endothelial Cell Markersmentioning

confidence: 99%

Novel endothelial cell markers in hepatocellular carcinoma

et al. 2004

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Hepatocellular carcinoma is characterized by hypervascularity and a propensity for vascular invasion. Detailed analysis of complementary DNA (cDNA) microarray global gene expression data and further validation on a smaller independent sample set by reverse transcription-polymerase chain reaction established the presence of two endothelial gene clusters in hepatocellular carcinoma. Cluster I, consists of 20 cDNA clones, representing 15 unique genes. Cluster II consists of nine unique genes. The expression of the cluster I genes appeared to be significantly upregulated in hepatocellular carcinoma compared with normal liver, cirrhotic liver, or nontumor liver tissues adjacent to the hepatocellular carcinoma. The pattern of gene expression of cluster I genes correlated positively with the 'proliferation gene cluster' and 'stromal cells cluster 2'. Expression of cluster II genes, in contrast, was not significantly different between hepatocellular carcinoma and non-neoplastic liver tissues. Studies conducted to localize the protein products of these genes by immunohistochemical staining of tissue arrays with up to 350 cores of tissues, and by in situ hybridization led to the discovery of novel sinusoidal endothelial cell markers in hepatocellular carcinoma: podocalyxin-like and regulator of G protein signaling-5. Our results underscore fundamental differences not only between neoplastic vs non-neoplastic liver cells but also between the hepatic sinusoidal endothelium of hepatocellular carcinoma and normal liver.

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“…We examined the distribution of human RAB9-like transcript in various human tissues by Northern blot analysis, as described previously (Seki et al 1998). The 961-bp cDNA fragment containing the entire open reading frame was used as a hybridization probe.…”

Section: Expression Profile Of Rab9-like Genementioning

confidence: 99%

cDNA cloning of a new member of the Ras superfamily, RAB9-like, on the human chromosome Xq22.1–q22.3 region

Seki¹,

Abe²,

Yoshikawa³

et al. 2000

J Hum Genet

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Members of the RAB protein family regulate vesicular trafficking and reside in specific intercellular compartments. A new member of the RAB family was identified through a public database search, and its fulllength cDNA was isolated from a human fetal brain cDNA library. The predicted protein product of the gene consists of 201 amino acid residues, and the protein has 86% similarity to human RAB9 at the amino acid level. We designated the new gene RAB9-like. Northern blot analysis showed that the gene was transcribed ubiquitously in various human tissues. A database search revealed that the gene is divided into three exons and spans approximately 7.2 kb of the genome DNA of chromosome Xq22.1-q22.3 region.

show abstract

Isolation, tissue expression, and chromosomal assignment of human RGS5, a novel G-protein signaling regulator gene

Cited by 50 publications

References 17 publications

Transcription Profiling of Platelet-Derived Growth Factor-B-Deficient Mouse Embryos Identifies RGS5 as a Novel Marker for Pericytes and Vascular Smooth Muscle Cells

Transcription Profiling of Platelet-Derived Growth Factor-B-Deficient Mouse Embryos Identifies RGS5 as a Novel Marker for Pericytes and Vascular Smooth Muscle Cells

Novel endothelial cell markers in hepatocellular carcinoma

cDNA cloning of a new member of the Ras superfamily, RAB9-like, on the human chromosome Xq22.1–q22.3 region

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