Isolation, synthesis, and drug interaction potential of secondary metabolites derived from the leaves of miracle tree (Moringa oleifera) against CYP3A4 and CYP2D6 isozymes

Khan, SI; Albadry, Mohamed A.; Parveen, Abidah; Hawwal, Mohammed F.; Majrashi, Taghreed A.; Ali, Zulfiqar; Chittiboyina, Amar G.; Khan, Ikhlas A.

doi:10.1016/j.phymed.2019.153010

Cited by 18 publications

(14 citation statements)

References 19 publications

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“…13 Prior studies reported the isolation of compound 1 from the methanol extract of M. oleifera [21,22]. In agreement with other research groups, we isolated this compound from the methanol extract of M. oleifera (#18332) in the previous isolation work [23]. However, compound 1 in the plant sample (#18332) and 13 herbal supplements in this analytical work were undetectable.…”

Section: Discussionsupporting

confidence: 87%

“…Secondary metabolites (1-5 and 7-9) were previously isolated and elucidated from the authenticated plant material #18332 at the National Center for Natural Products Research (NCNPR). Based on 1 H NMR data and HPLC chromatograms, the purity of phytoconstituents 2-5 and 7-9 was approximately 90-95 % while the purity for compound 1 was 85 % [23]. Compound 6 was purchased from Sigma-Aldrich with 85 % purity.…”

Section: Chemicals and Plant Samplesmentioning

confidence: 99%

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Profiling and Quantification of the Key Phytochemicals from the Drumstick Tree (Moringa oleifera) and Dietary Supplements by UHPLC-PDA-MS

et al. 2020

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Moringa oleifera is known as a drumstick tree and is cultivated in the subtropics and tropics. It exhibits antihypertensive and antidiabetic effects. An ultra-high-performance liquid chromatography method was developed for the determination of 9 phytochemicals in M. oleifera leaves and marketed products. The efficient separation was achieved within 7 min with a temperature of 45 °C by using a C-18 column as the stationary phase and water/acetonitrile with 0.05% formic acid as the mobile phase. The method was validated for linearity, repeatability, limits of detection, and limits of quantification. The limits of detections of phenolic compounds 1 – 9 were as low as 0.2 µg/mL. The photodiode array detector at 220 and 255 nm wavelengths was recruited for quantification. The key phytochemicals were detected in the range of 0.42 to 2.57 mg/100 mg sample weight in 13 dietary supplements. This study considers the quantitative analysis for lignans in M. oleifera for the first time. Isoquercitrin (5) and quercetin 3-O-(6-O-malonyl)-β−D-glucopyranoside (6) predominates the leaves of M. oleifera with inherent degradable nature detected for compound 6. Niazirin (2) was detected in amounts between 0.010 – 0.049 mg/100 mg while compound 1 was undetectable and potentially an artifact because of the fractionation process. The characterization and confirmation of components were achieved by liquid chromatography-electrospray ionization-mass spectrometry with extractive ion monitoring for the positive and negative ion modes. The developed and validated method is robust and rapid in the conclusive quantification of phytochemicals and authentication of the Moringa samples for quality assurance.

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Section: Discussionsupporting

confidence: 87%

Section: Chemicals and Plant Samplesmentioning

confidence: 99%

Profiling and Quantification of the Key Phytochemicals from the Drumstick Tree (Moringa oleifera) and Dietary Supplements by UHPLC-PDA-MS

et al. 2020

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“…Many drug–herb interactions are thought to result from the inhibitory effect on these CYP enzymes blocking the activation of cyclophosphamide [ 61 ]. Indeed, moringa extracts have been shown to inhibit liver CYP isozymes including CYP1A2 [ 62 ], CYP3A4 and CYP2D6 [ 63 ]. However, CYP enzymes constitute a superfamily of more than 50 isoforms responsible for both activating and detoxifying drugs.…”

Section: Discussionmentioning

confidence: 99%

Moringa Oleifera Seed Extract Concomitantly Supplemented with Chemotherapy Worsens Tumor Progression in Mice with Triple Negative Breast Cancer and Obesity

et al. 2021

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Triple negative breast cancer (TNBC) is an aggressive and highly metastatic breast cancer subtype with limited treatment options. Obesity and insulin resistance are associated with a worse prognosis in those with TNBC. Moringa oleifera (moringa) is a tropical edible plant used for both food and medicinal purposes and found to have anti-obesity and anti-cancer effects in vitro and in preclinical models. The anti-cancer effects of moringa seed extract alone and in combination with chemotherapy were evaluated in immunocompromised female mice with diet-induced obesity bearing MDA-MB-231-derived xenograft tumors. Moringa supplementation protected against high-fat diet- and chemotherapy-induced increases in fasting glucose and improved insulin sensitivity. Moringa supplementation alone did not attenuate tumor growth relative to chemotherapy alone, and in combination worsened tumor progression. Moringa supplementation alone reduced angiogenesis, but this effect was abrogated in combination with chemotherapy. Moringa supplementation may be an effective strategy to improve metabolic health in mice with obesity and TNBC and reduce angiogenesis in tumors, but may have a negative interaction when used as a concurrent complementary therapy. Caution should be taken when considering the consumption of moringa seed extracts while receiving chemotherapy for breast cancer treatment. Further investigations of alternative timings of moringa therapy are warranted.

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“…The consumption of these herbs may potentiate or antagonise the effects of some antihypertensive drugs, causing changes to the pharmacokinetics of the drug, primarily their metabolic activities [40]. Certain bioactive compounds and aqueous extracts of M. oleifera were determined to have CYP3A4 and CYP1A2 inhibitory properties respectively [41,42]. Hence, certain antihypertensive drugs such as amlodipine, is metabolised by CYP3A4 [43].…”

Section: Plos Onementioning

confidence: 99%

Self-management using crude herbs and the health-related quality of life among adult patients with hypertension living in a suburban setting of Malaysia

et al. 2021

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Purpose To determine the prevalence of crude herbs’ use in the self-management of hypertension and the health-related quality of life (HRQOL) in patients with hypertension. Methods This cross-sectional study was performed among patients with hypertension attending a government health clinic. Socio-demographic characteristics, lifestyle modifications, medical history and predictors of crude herbs users were obtained. The diversity of crude herbs used was assessed using a modified international complementary and alternative medicine questionnaire (I-CAM-Q) and the HRQOL was assessed using the SF36 instrument. Results Out of the 294 patients recruited, 52.4% were female, 41.5% were Malay and 38.8% were within the 60 to69 age category. The prevalence of crude herbs users was 30.6% and the most common herbs used were pegaga (Centella asiatica), peria (Momordica charantia) and betik (Carica papaya). Using the regression analysis, significantly higher odds of using crude herbs are noted among Malay or Indian patients who have these characteristics: attained secondary education, experienced falls or muscle pain, and had systolic blood pressure of more than 140 mmHg. There was no significant difference in HRQOL domains between the crude herb users and non-users (p>0.05). Conclusion Besides taking allopathic medications, certain patients with hypertension use crude herbs as a form of self-management. Although patients are adamant about integrating crude herbs as a form of self-management, the effects of doing so have not been properly investigated. This implies that the healthcare staff members need to communicate with the patients regarding the use of crude herbs together with conventional drugs.

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Isolation, synthesis, and drug interaction potential of secondary metabolites derived from the leaves of miracle tree (Moringa oleifera) against CYP3A4 and CYP2D6 isozymes

Cited by 18 publications

References 19 publications

Profiling and Quantification of the Key Phytochemicals from the Drumstick Tree (Moringa oleifera) and Dietary Supplements by UHPLC-PDA-MS

Profiling and Quantification of the Key Phytochemicals from the Drumstick Tree (Moringa oleifera) and Dietary Supplements by UHPLC-PDA-MS

Moringa Oleifera Seed Extract Concomitantly Supplemented with Chemotherapy Worsens Tumor Progression in Mice with Triple Negative Breast Cancer and Obesity

Self-management using crude herbs and the health-related quality of life among adult patients with hypertension living in a suburban setting of Malaysia

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