Isolation, Structure Determination, and Anti-HIV Evaluation of Tigliane-Type Diterpenes and Biflavonoid from Stellera chamaejasme

Abstract

Five novel tigliane-type diterpenes, stelleracins A–E (3–7), a novel flavanone dimer, chamaeflavone A (8), and six known compounds were isolated from roots of Stellera chamaejasme. Their structures were elucidated by extensive spectroscopic analyses. The isolated compounds were evaluated for anti-HIV activity in MT4 cells. New compounds 3–5 showed potent anti-HIV activity (EC90 0.00056–0.0068 μM) and relatively low or no cytotoxicity (IC50 4.4–17.2 μM). These new compounds represent promising new leads for development into anti-AIDS clinical trial candidates.

Ultra‐high performance liquid chromatography with linear ion trap‐Orbitrap hybrid mass spectrometry combined with a systematic strategy based on fragment ions for the rapid separation and characterization of components in Stellera chamaejasme extracts

“…In full scan mode, the centroid-type spectra were acquired in the m/z range 100-2 000. And in MS n experiments, data-dependent scanning was performed to trigger fragmentation spectra, which was set to select the strongest parent ions in each acquiring time points (four ions for MS 2 and one ion for MS 3 ) as targets for the next grade fragmentation. The dynamic exclusion function of the LTQ was enabled to prevent repetition on the same targeted ions and minimize the total analysis time.…”

De novo transcriptome analysis to identify flavonoid biosynthesis genes in Stellera chamaejasme

“…More and more studies have indicated that S. chamaejasme can synthesizes a group of flavonoids, which exhibit antibacterial, immunomodulation, anti-HIV, anti-HBV, antimitotic and antifungal activities (Asada et al, 2013;Liu et al, 2008;Xu et al, 2001;Yang et al, 2005). In addition, flavonoid is one of major floral pigments, and contributes most to the range and type of colored pigments in plants.…”

Diterpenoids from the stems of Tripterygium hypoglaucum (Celastraceae) and cytotoxic evaluation

“…Anti-HIV assay (Asada et al, 2013) MT4 cells were infected with HIV-1 NL4-3 (multiplicity of infection = 0.001) in the presence of various concentrations of compounds. Fresh medium, which contained appropriate concentrations of the compounds, was added to the culture 48 h after infection to maintain normal cell growth.…”