Isolation, Structural, and Functional Characterization of an Apoptosis-Inducing -Amino Acid Oxidase from Leaf-Nosed Viper (Eristocophis macmahoni) Snake Venom

Ali, Syed Abid; Stoeva, Stanka; Abbasi, Atiya; Alam, Junaid Mahmood; Kayed, Rakez; Faigle, Marion; Neumeister, Birgid; Voelter, Wolfgang

doi:10.1006/abbi.2000.2130

Cited by 98 publications

(72 citation statements)

References 28 publications

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“…In viperid, crotalid, and elapid snakes, amide oxidases have high enzymatic activities that are believed to contribute to toxicity upon envenomation through the production of hydrogen peroxide (27). They have been shown to induce human platelet aggregation (28), assist in hemorrhage (27) and apoptosis (29), and cause edema and hemolysis (30). We predict that the platypus protein would cause similar symptoms.…”

Section: Resultsmentioning

confidence: 99%

Proteomics and Deep Sequencing Comparison of Seasonally Active Venom Glands in the Platypus Reveals Novel Venom Peptides and Distinct Expression Profiles

Wong

Morgenstern

Mofiz

et al. 2012

Molecular & Cellular Proteomics

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Section: Resultsmentioning

confidence: 99%

Proteomics and Deep Sequencing Comparison of Seasonally Active Venom Glands in the Platypus Reveals Novel Venom Peptides and Distinct Expression Profiles

Wong

Morgenstern

Mofiz

et al. 2012

Molecular & Cellular Proteomics

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“…In 1994, Strizhkov et al reported that both neurotoxin II (NT II) from venom of Naja naja oxiana and 20-30 kDa proteins partially purified from pig brain (NTlm) cross-reacting with antibodies to NT II were cytotoxic to L929 and K562 tumor cells at concentrations of 10 -6 -10 -8 M, and induced apoptosis in L929 and K562 cells in vitro [34] . After that, L-amino acid oxidase (LAO) was found to induce apoptosis in human embryonic kidney cell line 293T [35] and human monocyte line MM6 [36] . Recently, Araki et al [37] and Masuda et al [38] associated integrins with vascular apoptosisinducing protein 1 (VAP1)-induced apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Serum from rabbit orally administered cobra venom inhibits growth of implanted hepatocellular carcinoma cells in mice

Sun¹,

Ren²,

Zhang³

et al. 2003

WJG

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AIM:To investigate the inhibitory effect of serum preparation from rabbits orally administered cobra venom (SRCV) on implanted hepatocellular carcinoma (HCC) cells in mice. METHODS:An HCC cell line, HepA, was injected into mice to prepare implanted tumors. The animals (n=30) were divided randomly into SRCV, 5-fluorouracil (5-FU), and distilled water (control) groups. From the second day after transplantation, 20 mg/kg 5-FU was administered intraperitoneally once a day for 9 days. SRCV (1 000 mg/kg) or distilled water (0.2 mL) was given by gastrogavage. Tumor growth inhibition was described by the inhibitory rate (IR). Apoptosis was detected by transmission electron microscopy (TEM), flow cytometry (FCM), and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL). Student's t-test was performed for statistical analysis. RESULTS:The tumor growth was inhibited markedly by SRCV treatment compared to that in the control group (P<0.01). The treatment resulted in a significant increase in the apoptotic rate of cancer cells by the factors of 10.5±2.4 % and 20.65±3.2 % as demonstrated through TUNEL and FCM assays, respectively (P<0.01). The apoptotic cells were also identified by characteristic ultrastructural features. CONCLUSION: SRCV can inhibit the growth of implantedHepA cells in mice, and the apoptosis rate appears to elevate during the process. Sun P, Ren XD, Zhang HW, Li XH, Cai SH, Ye KH, Li XK. Serum from rabbit orally administered cobra venom inhibits growth of implanted hepatocellular carcinoma cells in mice.

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“…Diverse studies have indicated that LAAOs have broad bioactivities such as inducing apoptosis [1], cytotoxicity [2,3], edema [4], hemolysis [5], hemorrhage [6], inducing or inhibiting platelet aggregation [7,8], parasite-killing activity [9], and antimicrobial activity [10]. LAAO may act as defence or attack weapons via H 2 O 2 [11] or as ideal molecular mechanisms for the acquisition of nitrogen from diverse amino acid sources [12].…”

Section: Nhmentioning

confidence: 99%

Isolation and Characterization of an L-Amino Acid Oxidase-Producing Marine Bacterium

Yu¹,

Qiao²,

Qiu³

et al. 2013

ENG

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One marine bacterial strain, R3, has been newly isolated from the intertidal zone of Dinghai sea area. Measurements of a-keto acids and H 2 O 2 existing in fermentation supernatant were carried out to show that R3 can produce L-amino acid oxidase (LAAO) with a broad substrate specificity. Physiological and biochemical analysis showed that it can grow great at the conditions with sodium chloride concentration of 1.5% -3%, temperature of 15˚C -35˚C and pH of 6 -7. In addition, molecular identification of 16S rDNA was performed to show that R3 was proximal to Pseudoalteromonas spp. with the highest identity of 98.5% to Pseudoalteromonas rubra. Therefore, it was designated as Pseudoalteromonas sp. R3. Further studies are required to arrive at a better understanding of this LAAO and secure an application.

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Isolation, Structural, and Functional Characterization of an Apoptosis-Inducing -Amino Acid Oxidase from Leaf-Nosed Viper (Eristocophis macmahoni) Snake Venom

Cited by 98 publications

References 28 publications

Proteomics and Deep Sequencing Comparison of Seasonally Active Venom Glands in the Platypus Reveals Novel Venom Peptides and Distinct Expression Profiles

Proteomics and Deep Sequencing Comparison of Seasonally Active Venom Glands in the Platypus Reveals Novel Venom Peptides and Distinct Expression Profiles

Serum from rabbit orally administered cobra venom inhibits growth of implanted hepatocellular carcinoma cells in mice

Isolation and Characterization of an L-Amino Acid Oxidase-Producing Marine Bacterium

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