Isolation, Storage, Pathotype Characterization, and Evaluation of Resistance for <i>Phytophthora sojae</i> in Soybean

Dorrance, Anne E.; Berry, Sue Ann; Anderson, Terry; Meharg, Chuck

doi:10.1094/php-2008-0118-01-dg

Cited by 116 publications

(143 citation statements)

References 12 publications

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“…The phenotype of an F 2:3 family was evaluated using the percentage of resistant progenies (survival-no expanding lesion). An F 2:3 family was considered homozygous resistant (R) if more than 70 % of the progenies survived, segregating (Rs) if 25-70 % survived and susceptible (S) if fewer than 25 % survived (Dorrance et al 2008;Sun et al 2011).…”

Section: Disease Evaluationmentioning

confidence: 99%

Molecular mapping of two genes conferring resistance to Phytophthora sojae in a soybean landrace PI 567139B

et al. 2013

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Phytophthora root and stem rot (PRR), caused by the soil-borne oomycete pathogen Phytophthora sojae, is one of the most destructive diseases of soybean. PRR can be effectively controlled by race-specific genes conferring resistance to P. sojae (Rps). However, the Rps genes are usually non-durable, as populations of P. sojae are highly diverse and quick to adapt, and can be overcome 8-15 years after deployment. Thus, it is important to identify novel Rps genes for development of resistant soybean cultivars. PI 567139B is a soybean landrace carrying excellent resistance to nearly all predominant P. sojae races in Indiana. A mapping population consisting of 245 F2 individuals and 403 F2:3 families was developed from a cross between PI 567139B and the susceptible cultivar 'Williams', and used to dissect the resistance carried by PI 567139B. We found that the resistance in PI 567139B was conferred by two independent Rps genes, designated RpsUN1 and RpsUN2. The former was mapped to a 6.5 cM region between SSR markers Satt159 and BARCSOYSSR_03_0250 that spans the Rps1 locus on chromosome 3, while the latter was mapped to a 3.0 cM region between BARCSOYSSR_16_1275 and Sat_144, approximately 3.0-3.4 cM upstream of Rps2 on chromosome 16. According to the 'Williams 82' reference genome sequence, both regions are highly enriched with NBS-LRR genes. Marker assisted resistance spectrum analyses of these genes with 16 isolates of P. sojae, in combination with the mapping results, suggested that RpsUN1 was likely to be a novel allele at the Rps1 locus, while RpsUN2 was more likely to be a novel Rps gene.

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Section: Disease Evaluationmentioning

confidence: 99%

Molecular mapping of two genes conferring resistance to Phytophthora sojae in a soybean landrace PI 567139B

et al. 2013

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“…The resistance scale was similar to that described by Dorrance et al (2008) for P. sojae race identification.…”

mentioning

confidence: 53%

“…The three single zoospore P. sojae isolates, PS6, PS17, and PS44, representing races 1, 3, and 5, respectively, used in this study were isolated from diseased soybean plants in 2008. Races of these isolates were confirmed by challenging them on a set of eight differential lines each containing a single resistance Rps gene and the universal suscept Williams (rpsrps).…”

mentioning

confidence: 99%

Reactions of Canadian short-season soybean cultivars to three races of Phytophthora sojae

Zhang¹,

Xue²,

Zhang³

et al. 2010

Can. J. Plant Sci.

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I. 2010. Reactions of Canadian short-season soybean cultivars to three races of Phytophthora sojae. Can. J. Plant Sci. 90: 207Á210. Of 87 short-season soybean cultivars evaluated for reactions to three races (1, 3, and 5) of Phytophthora sojae Kaufmann and Gerdemann, 29 showed resistance to at least one of the three races. These resistant sources may be used for pyramiding Rps genes and deployment of P. sojae resistant soybean cultivars in Canada.

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“…Recovery of P. sojae in the soil samples was conducted using the baiting method described by Canaday & Schmitthenner (1982), and isolation using the soybean seedling bioassay procedure described by Dorrance et al (2008). Each soil sample was ground to fine particles by hand and placed in plastic pots (10 cm diameter).…”

Section: Isolation Of P Sojaementioning

confidence: 99%

“…To isolate the pathogen, tissue sections (1 cm) cut from margin of lesions on hypocotyls were surface-sterilized in 0.25% sodium hypochlorite solution for 3 min, followed by 70% ethanol for 10 s, rinsed in sterilized distilled water, dried by blotting with sterile paper towels, and placed on the semi-selective PBNIC medium (Dorrance et al 2008) containing Terrachlor (99% pentachloronitrobenzene) at 0.04 g L −1 , Benlate (50% benomyl) at 0.01 g L −1 , neomycin sulphate at 0.10 g L −1 , Rovral (50% iprodione) at 0.04 g L −1 and chloramphenicol at 0.10 g L −1 in a V8 juice agar base. Hyphal tips of P. sojae that grew from the infected tissues were transferred 3 days after plating onto PBNIC medium for further purification.…”

Section: Isolation Of P Sojaementioning

confidence: 99%

Races ofPhytophthora sojaein Ontario, Canada, 2010–2012

Xue

Marchand

Chen

et al. 2015

Canadian Journal of Plant Pathology

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Phytophthora root rot (PRR), caused by the oomycete Phytophthora sojae, is a devastating disease of soybean worldwide, and has been present in Canada since the 1950s. Major resistance to P. sojae (Rps) genes have been deployed by soybean breeders to mitigate yield losses, which has caused shifts in the pathogen population to more complex pathotypes and the emergence of new races. This study reports on a survey of races and pathotypes of P. sojae throughout 203 commercial soybean fields and two PRR nurseries in the province of Ontario, Canada, and is the first extensive survey to be conducted in Canada since the late 1980s. A total of 22 races and one intermediate reaction type (IRT) were detected from 262 isolates from commercial soybean fields. Race 25 was the predominant race, comprising 43 isolates and representing 16% of the pathogen population. Races 3, 4, 5, 6, 7, 9, 28 and 45 were frequently detected, each representing 5-11% of the pathogen population. A total of 16 races and two IRTs were detected in 96 isolates from the two PRR nurseries. Race 3 was the most abundant, representing 23% of isolates, while races 5, 6, 7, 8, 9, 14, 25 and 28 each represented 5-10% of total isolates. New races and more complex pathotypes have emerged since the last Canadian survey, and this will have implications for the future deployment of Rps genes or other forms of resistance to P. sojae.Résumé: Le pourridié phytophthoréen des racines (PPR) du soja, causé par l'agent pathogène de la classe des oomycètes Phytophthora sojae, ravage le soja cultivé à l'échelle de la planète, et est présent au Canada depuis les années 1950. Des gènes de résistance verticale à P. sojae (Rps) ont été déployés par les sélectionneurs de soja afin de mitiger les pertes de rendement, ce qui a engendré des changements au sein des populations de l'agent pathogène, vers des profils de virulence plus complexe et l'émergence de nouvelles races. Dans le cadre de cette étude, un relevé des races et pathovars de P. sojae a été effectué à l'échelle de 203 champs de soja en production commerciale et deux pépinières d'études de cette maladie dans la province de l'Ontario, Canada, et représente le premier relevé de cette envergure dans cette région du Canada depuis la fin des années 1980. Au total, 22 races et un type de réaction intermédiaire (TRI) ont été identifiés parmi 262 isolats provenant de champs de soja en production commerciale. La race 25 s'est avérée prédominante, représentant 43 isolats ou 16% du total de la population de l'agent pathogène. Les races 3, 4, 5, 6, 7, 9, 28 et 45 ont été fréquemment identifiées, chacune représentant 5 à 11% du total. Un total de 16 races et deux TRIs furent identifiés au sein de 96 isolats provenant des deux pépinières d'études de la maladie. La race 3 était la plus abondante, représentant 23% des isolats, alors que les races 5, 6, 7, 8, 9, 14, 25 et 28 représentaient chacune entre 5 et 10% des isolats. De nouvelles races, ainsi que des pathovars plus complexes, ont fait leur apparition depuis le dernier relevé au C...

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Isolation, Storage, Pathotype Characterization, and Evaluation of Resistance for Phytophthora sojae in Soybean

Cited by 116 publications

References 12 publications

Molecular mapping of two genes conferring resistance to Phytophthora sojae in a soybean landrace PI 567139B

Molecular mapping of two genes conferring resistance to Phytophthora sojae in a soybean landrace PI 567139B

Reactions of Canadian short-season soybean cultivars to three races of Phytophthora sojae

Races ofPhytophthora sojaein Ontario, Canada, 2010–2012

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