Isolation, Screening and Production Studies of Uricase Producing Bacteria From Poultry Sources

Abstract

Uricase (urate oxidase EC 1.7.3.3) is a therapeutic enzyme that is widely used to catalyze the enzymatic oxidation of uric acid in the treatment of hyperuricemia and gout diseases. In this study, three bacterial species capable of producing extracellular uricase were isolated from a poultry source and screened based on the size of the clear zone using a uric acid agar plate. The bacterial species capable of producing uricase with the highest uricolytic activity was identified as Bacillus cereus strain DL3 using a 16SrRNA gene sequencing approach. The time-course study of uricase production was performed and the medium was optimized. Carboxymethylcellulose and asparagine were found to be the best carbon and nitrogen sources. Maximum uricolytic activity was observed at pH 7.0 with an inducer concentration of 2.0 g/L. Inoculum size of 5% gave maximum uricolytic activity. The maximum uricolytic activity of 15.43 U/mL was achieved at optimized conditions, which is 1.61 times more than the initial activity. Further, enzymatic stability was determined at different pH and temperature.

Ureide metabolism in plant-associated bacteria: purine plant-bacteria interactive scenarios under nitrogen deficiency

“…However, in S. coelicolor as well as in the soil and plant colonizing Listeria monocytogenes, the XDH is involved mainly in the purine salvage catabolic pathway needed to generate sufficient GDP and GTP, the substrates for the phosphorylated nucleosides guanosine 5′(di)triphosphate 3′diphosphate synthetases (Sivapragasam and Grove 2016), via the XDH transcriptional repressor (xdhR) with a binding site to either GTP or ppGpp transcriptional repressor to induce xdhABC expression. As far as we could verify, there is only one publication on the inhibitory effects of plant phenolics and flavonoids from Juniperus procera on the XO activity in Gram-positive bacteria (Samaha et al 2017), contrasting with the large number of clinical reports showing the inhibitor effects of plant isoflavones and flavonol glycosides on the XO and/or UOX activities in bacteria pathogens to animals and humans (Nile et al 2017;Raziq et al 2017 (Nanda and Jagadeesh Babu 2014). In general, UOX was categorized as a thermosensitive and co-factorindependent enzyme.…”

Selective inhibition of adenylyl cyclase subtype 1 reduces inflammatory pain in chicken of gouty arthritis

“… 2 , 18 , 19 Chicken is considered to be one of the ideal animal models of gout due to lack of uricase. 3 , 4 In the present work, we injected MSU into the left ankle joint of chickens to establish a gouty arthritis model. We found that the ankle joint of chickens generated spontaneous pain behaviors for several hours by injection of MSU, and the left ankle joint was showed pain hypersensitivity for mechanical stimulus.…”

“…Particularly, both of them lack uricase. 3 , 4 Different from the high cost of the uricase-deficient mouse model and the complexity of model construction, the establishment of the chicken model of gouty arthritis has advantages in the similarity of the uric acid metabolism pathway and the simplicity of operation. 5 , 6 Therefore, chicken is an ideal animal for studying gouty arthritis.…”

Isolation of Bile Salt Hydrolase and Uricase Producing Lactobacillus brevis SF121 from Pak Sian Dong (Fermented Spider Plant) for using as Probiotics

“…To determine uricase activity, plate assay with modification was performed 23,25 . The isolate was streaked on MRS agar containing 0.5% (w/v) uric acid (Sigma-Aldrich, USA) and clear zone were observed after incubation for 48 -72 h at 37°C.…”

“…Therefore, catalytic for uric acid activity, uricase is interested to be used for hyperuricemia and gout. Variety genus of bacteria have been screened and isolated with uricase activity, such as Bacillus 23,24 , Pseudomonas 25 and Lactobacillus 26 .…”