Isolation, Screening and Optimized Production of Extracellular Xylanase under Submerged Condition from Aspergillus Flavus Mtcc 9390

Bhushan, Bharat; Pal, Ajay; Jain, Veena

doi:10.4172/2329-6674.1000103

Cited by 4 publications

References 35 publications

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Improved Enzyme Catalytic Characteristics upon Glutaraldehyde Cross-Linking of Alginate Entrapped Xylanase Isolated fromAspergillus flavusMTCC 9390

2015

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Purified fungal xylanase was entrapped in alginate beads. Its further cross-linking using glutaraldehyde resulted in large enzyme aggregates which may function as both a catalyst and a support material for numerous substrate molecules. Enzyme cross-linking presented a negative impact on enzyme leaching during repeated washings and recovery of enzyme activity was substantial after twelve cycles of usage. The entrapment followed by cross-linking doubled the total bound activity and also greatly improved the enzyme stability at extreme chemical environment. The wide pH stability, better thermo- and storage stability, lowered K m value, and protection from some metal ions are salient achievements of present immobilization. The study shows the efficacy, durability, and sustainability of immobilized catalytic system which could be efficiently used for various juice processing operations.

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Improved Enzyme Catalytic Characteristics upon Glutaraldehyde Cross-Linking of Alginate Entrapped Xylanase Isolated fromAspergillus flavusMTCC 9390

2015

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Development of Novel Pectinase and Xylanase Juice Clarification Enzymes via a Combined Biorefinery and Immobilization Approach

Hassan¹

2020

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Optimisation of Medium Formulation and Growth Conditions for Xylanase Production by Aspergillus brasiliensis in Submerged Fermentation (SmF)

Hood²

2014

J Biodivers Biopros Dev

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Objective: There has been much concern and immense importance on xylanase due to its potential industrial applications in the manufacturing of pulp and paper, baking, food and beverages, textile and animal feeds production. There are continuously endeavors to turn xylanase into a profitable market with lower production costs using optimum fermentation medium and growth conditions. Thus, the main objectives of this study were to determine the optimum medium formulation and growth conditions for the production of xylanase by Aspergillus brasiliensis ATCC 16404 and to elucidate the correlation of xylanase synthesis with biomass, pH and protein concentration in submerged fermentation (SmF). Methods:The effect of various carbon sources: sucrose, glucose, maltose, galactose, glycerol, lactose and ethanol and nitrogen sources: yeast extract, urea, peptone, ammonium sulphate, ammonium nitrate, ammonium chloride and sodium nitrate on xylanase activity were examined to obtain the optimum medium formulation for xylanase activity by A. brasiliensis. In addition, growth temperatures of 28 to 36 o C and initial pH medium of 3 to 11 were also examined to elucidate the optimum growth conditions for the production of xylanase. Results:The highest xylanase activity of 39.567 U/mL at 72 h was obtained using sucrose and yeast extract as the optimised carbon and nitrogen sources. Our results also showed that 30°C and pH 6.5 were the optimum growth condition of A. brasiliensis for xylanase synthesis. 38.528 U/mL of xylanase activity was observed at 72 h when the initial pH medium was adjusted to 5.0. Nevertheless, when the initial pH medium was increased to 6.5, the xylanase synthesis increased almost 3% at 72 h. Conclusion:Hence, in our study, the optimum medium formulation and growth conditions for the production of xylanase by A. brasiliensis in SmF was achieved by growing the fungi in medium containing sucrose and yeast extract with the initial medium pH 6.5 at 30°C.

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Isolation, Screening and Optimized Production of Extracellular Xylanase under Submerged Condition from Aspergillus Flavus Mtcc 9390

Cited by 4 publications

References 35 publications

Improved Enzyme Catalytic Characteristics upon Glutaraldehyde Cross-Linking of Alginate Entrapped Xylanase Isolated fromAspergillus flavusMTCC 9390

Improved Enzyme Catalytic Characteristics upon Glutaraldehyde Cross-Linking of Alginate Entrapped Xylanase Isolated fromAspergillus flavusMTCC 9390

Development of Novel Pectinase and Xylanase Juice Clarification Enzymes via a Combined Biorefinery and Immobilization Approach

Optimisation of Medium Formulation and Growth Conditions for Xylanase Production by Aspergillus brasiliensis in Submerged Fermentation (SmF)

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