Isolation, screening and molecular characterization of biosurfactant producing bacteria from soil samples of auto repair shops

Uyar, Ebru; Sağlam, Özgür

doi:10.1007/s00203-021-02483-4

Cited by 18 publications

(5 citation statements)

References 47 publications

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“…Additionally, the construction of the evolutionary tree was confirmed by rechecking the species’ similarity and dissimilarity using bootstrap techniques with 1000 replications. The maximum composite likelihood technique was utilized to compute distances …”

Section: Methodsmentioning

confidence: 99%

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Isolation, Screening, Characterization, and Optimization of Biosurfactant-Producing Bacteria for Removal of Micropollutants from Textile Effluents

Kumari,

Debnath,

Joshi

et al. 2024

Ind. Eng. Chem. Res.

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Biosurfactants are ecofriendly substitutes for chemical surfactants. In this study, biosurfactant-producing bacterial strains were isolated from soil and water samples from textile industry sites. Extracted biosurfactants could reduce the surface tension values from 72 to 39.3 mN/m demonstrating that the biosurfactants achieved the critical micelle concentration. Stable emulsifications with diesel and vegetable oil showed an emulsification index ranging from 61.76% to 76.47%. The presence of both rhamnolipid and surfactin types of biosurfactants was confirmed from our isolates using FTIR, LC-MS/MS, and GC-MS/MS analyses. These biosurfactant-producing bacteria act on dyes and heavy metals present in textile wastewater and reduce their concentrations by either degradation or biosorption. After the reaction with biosurfactant-producing bacterial isolates, FTIR spectra confirmed the breakdown of some compounds and their conversion into intermediates. There was also a reduction in the concentration of metals in textile effluents, as shown by EDAX analysis. These novel bacteria can be scaled up for their use in water reclamation and recycled for regular industrial use.

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Section: Methodsmentioning

confidence: 99%

“…The maximum composite likelihood technique was utilized to compute distances. 39 2.7. Bacterial Growth Analysis.…”

Section: Sample Collectionmentioning

confidence: 99%

Isolation, Screening, Characterization, and Optimization of Biosurfactant-Producing Bacteria for Removal of Micropollutants from Textile Effluents

Kumari,

Debnath,

Joshi

et al. 2024

Ind. Eng. Chem. Res.

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“…Combined with short-read nextgeneration sequencing (NGS), 16S rDNA gene amplicon-based analysis of bacterial communities is possible and relatively straightforward 2 . At present, 16S rDNA sequencing has been widely incorporated into various fields of science, including food analysis [3][4][5] , environmental surveys 6,7 , and clinical tests [8][9][10][11] .…”

Section: Extension Of Bacterial Rdna Sequencing For Simultaneous Meth...mentioning

confidence: 99%

Extension of bacterial rDNA sequencing for simultaneous methylation detection and its application in microflora analysis

Nishimura

Tanaka

Murata

et al. 2023

Sci Rep

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Although polymerase chain reaction (PCR) amplification and sequencing of the bacterial 16S rDNA region has numerous scientific applications, it does not provide DNA methylation information. Herein, we propose a simple extension for bisulfite sequencing to investigate 5-methylcytosine residues in the bacterial 16S rDNA region from clinical isolates or flora. Multiple displacement amplification without DNA denaturation was used to preferentially pre-amplify single-stranded bacterial DNA after bisulfite conversion. Following the pre-amplification, the 16S rDNA region was analyzed using nested bisulfite PCR and sequencing, enabling the simultaneous identification of DNA methylation status and sequence data. We used this approach (termed sm16S rDNA PCR/sequencing) to identify novel methylation sites and a methyltransferase (M. MmnI) in Morganella morganii and different methylation motifs among Enterococcus faecalis strains from small volumes of clinical specimens. Further, our analysis suggested that M. MmnI may be correlated to erythromycin resistance. Thus, sm16S rDNA PCR/sequencing is a useful extension method for analyzing the DNA methylation of 16S rDNA regions in a microflora, providing additional information not provided by conventional PCR. Given the relationship between DNA methylation status and drug resistance in bacteria, we believe this technique can be effectively applied in clinical sample testing.

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“…The study of speci c pollutant-degrading bacterial species to determine their degradation capacities and associated metabolic pathways requires the isolation of bacterial colonies. Currently, bacterial isolation of PAHs and TNT degrader-species is commonly done through bacterial enrichment in liquid media containing the pollutant of interest, for example motor oil (Uyar & Sağlam, 2021). This strategy has proven useful to our group for the isolation of PAH-degrading bacteria, such as Sphingobium xenophagum D43FB (Gran-Scheuch et al, 2017, p. 2) and TNT-degrading bacteria, such as the isolates TNT3, TNT11 and TNT19, of the Pseudomonas genus (Cabrera et al, 2020(Cabrera et al, , 2022.…”

Section: Introductionmentioning

confidence: 99%

Solid medium for the direct isolation of bacterial colonies growing with polycyclic aromatic hydrocarbons or 2,4,6-trinitrotoluene (TNT)

et al. 2023

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Isolation of hydrocarbon-degrading bacteria is a key step for the study of microbiological diversity, metabolic pathways and bioremediation, however current methods lack simplicity and versatility. We developed an easy method that allows the screening and isolation of bacterial colonies capable of degrading hydrocarbons, such as diesel or polycyclic aromatic hydrocarbons (PAHs), as well as the pollutant explosive, 2, 4, 6-trinitrotoluene (TNT). The method uses a two-layer solid medium, with a layer of M9 medium, with a second layer containing the carbon source deposited trough the evaporation of ethanol. Using this medium we grew hydrocarbon-degrading strains, using diesel, phenanthrene or anthracene as the sole carbon sources, as well as three TNT-degrading isolates. Using this medium we isolated PAHs-degrading bacterial colonies directly from diesel polluted soils. Analysis revealed that bacteria grown in medium using PAHs as carbon source maintain their morphological characteristics when compared to cells grown on traditional media with glucose.

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Isolation, screening and molecular characterization of biosurfactant producing bacteria from soil samples of auto repair shops

Cited by 18 publications

References 47 publications

Isolation, Screening, Characterization, and Optimization of Biosurfactant-Producing Bacteria for Removal of Micropollutants from Textile Effluents

Isolation, Screening, Characterization, and Optimization of Biosurfactant-Producing Bacteria for Removal of Micropollutants from Textile Effluents

Extension of bacterial rDNA sequencing for simultaneous methylation detection and its application in microflora analysis

Solid medium for the direct isolation of bacterial colonies growing with polycyclic aromatic hydrocarbons or 2,4,6-trinitrotoluene (TNT)

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