Isolation, Screening and Identification of Biosurfactant-producing Bacteria from Hydrocarbon-polluted and Pristine Soils within Ogoniland, Nigeria

Nwaguma, I. V.; Chikere, Chioma Blaise; Okpokwasili, G. C.

doi:10.9734/bmrj/2016/26294

Cited by 3 publications

(3 citation statements)

References 18 publications

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“…The hemolytic activity demonstrated by the strain was superior to that presented by Aspergillus species MSF1 that caused the appearance of a clear zone with a diameter of 7 mm in blood agar medium as described by Kiran et al (2010). The hemolytic activity was employed by several authors as initial criterion for selection of biosurfactant producers (Batool et al, 2017;Nwaguma et al, 2016). However, Satpute et al (2010), recommend the use of more than one screening method for detection of biosurfactantproducing microorganisms.…”

Section: Detection Of Biosurfactant-producing Rhizopus Strainsmentioning

confidence: 96%

“…However, the majority of screening studies have been carried out using bacteria (Kebbouche-Gana et al, 2009;Nwaguma et al, 2016;Joy et al, 2017;Batool et al, 2017), and outgrows by far those evaluating the fungi producing potential (Sari et al, 2014;Lodha et al, 2016). Till date however, the need for discovery of biosurfactant producing microorganisms capable of inhabiting environments featured by adverse typical conditions such as extreme salinity, higher temperatures, and scanty humidity is still enormous (Techaoei et al, 2007;Kebbouche-Gana et al, 2009;Kiran et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Development and improved selected markers to biosurfactant and bioemulsifier production by Rhizopus strains isolated from Caatinga soil

Pele¹,

Rodríguez²,

Ribeaux³

et al. 2018

Afr. J. Biotechnol.

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This study screened four Rhizopus species as biosurfactant producers using different markers. First of all, Rhizopus spp. UCP 1607 was identified as Rhizopus arrhizus by morphological and molecular methods. The production of biosurfactant/bioemulsifier was investigated by submerged fermentation using soybean post-frying oil (5% v/v) and sodium glutamate (1% w/v) medium. The primary markers' hemolysis and parafilm M tests showed that R. arrhizus UCP1607 strain exhibited higher hemolytic activity (49 mm of clear zone) on sheep blood agar and a larger drop diameter (12 mm) on parafilm hydrophobic surface. The experimental results showed the most promising biosurfactant production by R. arrhizus UCP 1607 strain led to a reduction of surface tension (31.8 mN/m) and the diameter of the oil-spreading covered an area of 66.4 cm 2 . The strains Rhizopus microsporus var. chinensis UCP1296, R. microsporus var. microsporus UCP1304, and R. arrhizus UCP1607 were capable of forming stable emulsions corresponding to 91.7, 94.8, and 82.6%, respectively in crude oil.

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Section: Detection Of Biosurfactant-producing Rhizopus Strainsmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Development and improved selected markers to biosurfactant and bioemulsifier production by Rhizopus strains isolated from Caatinga soil

Pele¹,

Rodríguez²,

Ribeaux³

et al. 2018

Afr. J. Biotechnol.

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“…Much research is conducted to identify biosurfactant-producing bacteria from different environments in contact with different hydrocarbon oils mainly for the purpose of bioremediation and biodegradation as well as enhanced oil recovery (Christova et al, 2019;Cai et al, 2017;Nwagumal et al, 2016;Souza et al, 2014;Bach & Gutnick, 2004). However, fewer reports are available on identification of biosurfactant-producing bacteria and their role in hydrocarbon bioprocessing applications such as viscosity reduction and heavy oil upgrading (Gudiña & Teixeira, 2017;Cerón-Camacho et al, 2013;Perfumo et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification of the same type LMG1242 of biosurfactant-producing strain of Pseudomonas aeruginosa from different oil contaminated soils

Ghavipanjeh

Moradi

2019

APJMBB

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Emulsification is a major rate limiting step in any biological conversion of heavy hydrocarbons. Here, thirty bacterial strains were isolated from seven native consortia and screened for biosurfactantproducing activities which could enhance emulsification of heavy oils. The consortia were obtained by sampling from oil contaminated soils of different petroleum refineries of Iran. The oil spreading test, drop collapse test, emulsification index (E24) and surface tension measurements were used to evaluate the biosurfactant producing activities of the strains. A total number of 5 strains out of 30 were finally selected as the best biosurfactant-producing bacteria. The clear zone diameters in their oil spreading test were 4 to 5 cm, and E24 were 48 to 92%. The selected strains also properly lowered the surface tensions of the supernatants to 23.5-32 mN/m when grown on heavy diesel. All the selected strains were identified by 16S rRNA sequencing analysis as Pseudomonas aeruginosa LMG1242 (99.9% homology) which is a newly isolated type in oil polluted soils. The results suggest P. aeruginosa LMG1242 as a predominant and highly active biosurfactant producing bacterium which could be further evaluated in petroleum bioremediation and bioprocessing applications.

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Screening and Characterization of Biosurfactant-Producing Bacillus Species Isolated from Contaminated Soils in Makurdi Metropolis

John¹,

Ogbonna²,

Gberikon³

et al. 2021

Nig. J. Biotechnol

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Biosurfactants synthesized by microorganisms are chemically diverse and have gained interest industrially due to their surface and interfacial tensions-reducing activities. In this study Bacillus species from contaminated soils were screened and characterized for biosurfactant production. The study was carried out at the Microbiology Laboratory, Federal University of Agriculture Makurdi, Nigeria. The Bacillus species were isolated from kerosene shops, palm oil shops, nearby restaurants, mechanic workshops and abattoir effluents- contaminated soil samples collected from Makurdi metropolis. The Bacillus spp. were screened for biosurfactants production potentials using various screening methods (oil spreading, beta haemolysis, drop collapse and emulsification index). Specific primers were used to amplify the srfAA (surfactin gene) gene in the Bacillus isolates and the nucleotide sequences were determined at Inqaba Biotec, South Africa. The screening results were statistically analysed using analysis of variance (ANOVA) at 95 % confidence level. Isolate RT7(4)B exhibited the ability to produce biosurfactant, as well as the highest emulsification index (E24) of 73.25 % while isolate PO7(3)C gave the highest oil displacement of 6.77 mm. The supernatant obtained from isolate RT7(4)B showed reduction in surface tension of up to 30.26 mN/m. The isolates gave positive results for biosurfactant production when subjected to drop collapse and Beta haemolytic tests. The Polymerase chain reaction (PCR) results revealed amplifications of srfAA gene from 7 isolates. Based on these findings, the isolates used in this study can be utilized for biosurfactant production, and can also be useful for bioremediation and industrial biotechnology applications. Keywords: Biosurfactants; emulsification index; Bacillus; surface tension; Drop collapse

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Isolation, Screening and Identification of Biosurfactant-producing Bacteria from Hydrocarbon-polluted and Pristine Soils within Ogoniland, Nigeria

Cited by 3 publications

References 18 publications

Development and improved selected markers to biosurfactant and bioemulsifier production by Rhizopus strains isolated from Caatinga soil

Development and improved selected markers to biosurfactant and bioemulsifier production by Rhizopus strains isolated from Caatinga soil

Isolation and identification of the same type LMG1242 of biosurfactant-producing strain of Pseudomonas aeruginosa from different oil contaminated soils

Screening and Characterization of Biosurfactant-Producing Bacillus Species Isolated from Contaminated Soils in Makurdi Metropolis

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