Isolation, purification, immunological and anti-tumor activities of polysaccharides from Gymnema sylvestre

Wu, Xiangyang; Mao, Guanghua; Fan, Q; Zhao, Ting; Zhao, Jun; Li, Fang

doi:10.1016/j.foodres.2012.02.006

Cited by 45 publications

(39 citation statements)

References 9 publications

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“…These assignments of gymnemagenin were supported by comparison of their linear retention indices calculated using standard with those reported in the literature [5,38,39]. The GC spectrum confirmed the extracted bioactive molecule as gymnemic acid and the inserts with in the image shows the standard gymnemagenin.…”

Section: Resultssupporting

confidence: 77%

In vivo anti-ulcer, anti-stress, anti-allergic, and functional properties of Gymnemic Acid Isolated from Gymnema sylvestre R Br

Arun

Arunachalam

et al. 2014

BMC Complement Altern Med

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BackgroundGymnema sylvestre is a highly valued ethno pharmacologically important medicinal plant used currently in many poly-herbal formulations due to its potential antidiabetic activity and other health benefits. The present study was carried out to analyze the anti-stress, anti-allergic, and antiulcer activity of the bioactive compounds present in Gymnema sylvestre leaves.MethodsThe preliminary phytochemical screening for bioactive compounds from aqueous extracts revealed the presence of alkaloids, triterpenes, flavonoids, steroids, and saponins. The antioxidant activities were investigated using DPPH radical scavenging method. The characterization of the extract was carried out using standard compound by High Performance Thin Layer Chromatography (HPTLC) and phytochemical analysis in terms of total phenol, total flavonoids, reducing power and antioxidant potentials, etc. The in vivo studies on albino mice proved the purified fraction has anti-stress/anti-allergic activity against milk induced leucocytosis/eosinophilia and able to inhibit the aspirin induced gastric ulcers.ResultsThe quantitative estimation for aqueous extract exhibited total antioxidant (9.13 ± 0.04 μg/g), flavonoids (125.62 ± 26.84 μg/g), tannin (111.53 ± 15.13 μg/g), total phenol content (285.23 ± 1.11 μg/g) and free radical scavenging (52.14 ± 0.32%). Further the aqueous extract was consecutively purified by TLC and silica column chromatography. The purified fractions were characterized by HPTLC and GC-MS and the component was identified as gymnemic acid. The potency of the antimicrobial activity of the extract was studied with bacteria. Pharmacological experiments clearly demonstrated that the extracts of all plants given orally showed significant gastric protection against the asprin-induced gastric ulcer model in mice. Furthermore, healing effects were also confirmed through histopathological examination.ConclusionsThe aqueous extracts of the leaves of Gymnema sylvestre possess anti ulcerogenic, Anti allergic, Anti stress, properties that may be due to cytoprotective mechanism. These results support the ethno medical uses of the plant in the treatment of gastric ulcer.

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Section: Resultssupporting

confidence: 77%

In vivo anti-ulcer, anti-stress, anti-allergic, and functional properties of Gymnemic Acid Isolated from Gymnema sylvestre R Br

Arun

Arunachalam

et al. 2014

BMC Complement Altern Med

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“…The former is mainly responsible for cellular immunity, and the latter is the only cell capable of producing antibodies [27]. Many reports confirmed that polysaccharides exerted their antitumor effect through enhancing the immune system of the host animal [28][29][30]. The immunologic action of polysaccharides may begin with the activation of effector cells such as lymphocytes and macrophages [19].…”

Section: Splenocyte Proliferationmentioning

confidence: 99%

Purification, characterization and biological activity of a novel polysaccharide from Inonotus obliquus

Chen

Huang

Cui

et al. 2015

International Journal of Biological Macromolecules

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“…2 The use of plant parts and isolated phytochemicals for the prevention and treatment of various health ailments has been in practice for many decades. 3 In addition, it also possesses antimicrobial, 9 antitumor, 5 anti-obesity, 10 anti-inflammatory, 11 anti-hyperglycemic, 12 antiulcer, anti-stress, and antiallergic activity. 13 The presence of flavonoids, saponins, anthraquinones, quercitol, and other alkaloid have been reported in the flowers, leaves, and fruits of G. sylvestre.…”

Section: Introductionmentioning

confidence: 99%

Potential anticancer properties of bioactive compounds of Gymnema sylvestre and its biofunctionalized silver nanoparticles

Arunachalam¹,

Arun²,

Annamalai³

et al. 2014

IJN

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Background Gymnema sylvestre is an ethno-pharmacologically important medicinal plant used in many polyherbal formulations for its potential health benefits. Silver nanoparticles (SNPs) were biofunctionalized using aqueous leaf extracts of G. sylvestre . The anticancer properties of the bioactive compounds and the biofunctionalized SNPs were compared using the HT29 human adenoma colon cancer cell line. Methods The preliminary phytochemical screening for bioactive compounds from aqueous extracts revealed the presence of alkaloids, triterpenes, flavonoids, steroids, and saponins. Biofunctionalized SNPs were synthesized using silver nitrate and characterized by ultraviolet–visible spectroscopy, scanning electron microscopy, energy-dispersive X-ray analysis, Fourier transform infrared spectroscopy, and X-ray diffraction for size and shape. The characterized biofunctionalized G. sylvestre were tested for its in vitro anticancer activity against HT29 human colon adenocarcinoma cells. Results The biofunctionlized G. sylvestre SNPs showed the surface plasmon resonance band at 430 nm. The scanning electron microscopy images showed the presence of spherical nanoparticles of various sizes, which were further determined using the Scherrer equation. In vitro cytotoxic activity of the biofunctionalized green-synthesized SNPs (GSNPs) indicated that the sensitivity of HT29 human colon adenocarcinoma cells for cytotoxic drugs is higher than that of Vero cell line for the same cytotoxic agents and also higher than the bioactive compound of the aqueous extract. Conclusion Our results show that the anticancer properties of the bioactive compounds of G. sylvestre can be enhanced through biofunctionalizing the SNPs using the bioactive compounds present in the plant extract without compromising their medicinal properties.

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Isolation, purification, immunological and anti-tumor activities of polysaccharides from Gymnema sylvestre

Cited by 45 publications

References 9 publications

In vivo anti-ulcer, anti-stress, anti-allergic, and functional properties of Gymnemic Acid Isolated from Gymnema sylvestre R Br

In vivo anti-ulcer, anti-stress, anti-allergic, and functional properties of Gymnemic Acid Isolated from Gymnema sylvestre R Br

Purification, characterization and biological activity of a novel polysaccharide from Inonotus obliquus

Potential anticancer properties of bioactive compounds of Gymnema sylvestre and its biofunctionalized silver nanoparticles

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