Isolation, purification and partial characterization of plantaricin 423, a bacteriocin produced by Lactobacillus plantarum

Abstract

Lactobacillus plantarum 423, isolated from sorghum beer, produces a bacteriocin (plantaricin 423) which is inhibitory to several food spoilage bacteria and food-borne pathogens, including Bacillus cereus, Clostridium sporogenes, Enterococcus faecalis, Listeria spp. and Staphylococcus spp. Plantaricin 423 is resistant to treatment at 80 degrees C, but loses 50% of its activity after 60 min at 100 degrees C and 75% of its activity after autoclaving (121 degrees C, 15 min). Plantaricin 423 remains active after incubation at pH 1-10 and is inactivated when treated with pepsin, papain, alpha-chymotrypsin, trypsin and Proteinase K. Plantaricin 423 was partially purified and its size estimated at 3.5 kDa, as determined by tricine-SDS-PAGE. The mechanism of activity of plantaricin 423 is weakly bactericidal, as determined against Oenococcus oeni (previously Leuconostoc oenos). High DNA homology was obtained between the plasmid DNA of strain 423 and the pediocin PA-1 operon of Pediococcus acidilactici PAC 1.0, suggesting that plantaricin 423 is plasmid-encoded and related to the pediocin gene cluster.

Expression of different types of [FeFe]-hydrogenase genes in bacteria isolated from a population of a bio-hydrogen pilot-scale plant

“…In this case, the coexistence of Lactobacillus plantarum and Enterococcus species with various Clostridium species suggests that the strains isolated here interact favourably between them and, conversely, that the bacteriocins potentially produced by L. plantarum [59,62] and Enterococcus sp. C. beijerinckii SM10 shows the most complex behaviour, with two genes constitutively expressed in all the four time points: Cbei_0327 (M3a' structure) and Cbei_4110 (TR(M3) structure).…”

“…It is also interesting to consider that Lactobacillus plantarum produces various bacteriocins that can inhibit the growth of several microorganisms, including Clostridium, Enterococcus and Staphylococcus species; importantly, many strain-to-strain differences have been reported [59,[62][63][64]. Also various Enterococcus species are reported to produce bacteriocins [60,65].…”

Comparison of two methods for purification of plantaricin ST31, a bacteriocin produced by Lactobacillus plantarum ST31

“…A number of L. plantarum strains produce bacteriocins, many of which have been isolated and partially characterized (2,6,9,10,14,16).…”

NMR solution structure of the precursor for carnobacteriocin B2, an antimicrobial peptide from Carnobacterium piscicola

“…For example, Fimland et al [42] have shown that interchange of large domains of different type IIa bacteriocins, such as pediocin PA-1, sakacin P, and curvacin A, gives chimeric peptides, the antimicrobial specificity of which for target organisms corresponds to the C-terminal region. It has also been demonstrated that a C-terminal fragment of pediocin (residues [20][21][22][23][24][25][26][27][28][29][30][31][32][33][34] have not yet been performed with prebacteriocins, the present work shows that the leader peptide does not interfere with formation of the key C-terminal a-helix, and as a result, all of the precursors are likely to possess significant antimicrobial activity with specificity similar to the mature type IIa bacteriocin.…”