Isolation, purification and characterization of naturally derived Crocetin beta-d-glucosyl ester from Crocus sativus L. against breast cancer and its binding chemistry with ER-alpha/HDAC2

Mir, Mudasir Ahmad; Ganai, Shabir Ahmad; Mansoor, Sheikh; Jan, Sumira; Mani, P.; Masoodi, Khalid Z.; Amin, Henna; Rehman, Muneeb U.; Ahmad, Parvaiz

doi:10.1016/j.sjbs.2020.01.018

Cited by 43 publications

(30 citation statements)

References 44 publications

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“…The immense properties of this spice marks it as a red Gold for any country capable of growing this evocative spice as its commercialization would fetch tangible profits, that would in turn help in uplifting the socio-economic conditions of people and would pave a way to leverage the economy. There are numerous properties attributed to this delicate golden crop ranged including therapeutic medicinal benefits and capacity to be an agent for pharmaceutical industry ( Ferrence and Bendersky, 2004 , Hosseini et al, 2018 , Mir et al, 2020 , Nemati et al, 2014 , Razak et al, 2017 ). However, there are multitude of challenges which the current saffron industry is facing and to overcome these challenges to an extent, biotechnological interventions could play a crucial role to ameliorate this precious crop at the molecular level such as disease, abiotic stress resistant cultivars or cultivars with superior chemical profiling etc.…”

Section: Discussionmentioning

confidence: 99%

Deciphering genetic diversity analysis of saffron (Crocus sativus L.) using RAPD and ISSR markers

Mir

Mansoor

Sugapriya

et al. 2021

Saudi Journal of Biological Sciences

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The existence of genetic diversity in Crocus sativus has globally remained a mystery till date. The study investigated PCR based DNA amplification profile of saffron using ISSR and RAPD based primers. A total of 38 amplicons were generated by ISSR primers in the range from 7 to 12 with an average of 9.50 bands per primer. 20 bands were found to be polymorphic and 18 were monomorphic with an average percentage of polymorphism as 52.48%. RAPD based amplification revealed a total 161 amplicons, 107 as polymorphic and 54 as monomorphic with an average percentage of polymorphism as 66.44%. Cumulative results of RAPD and ISSR demonstrated that Nei-Li’s similarity index ranged between 0.70 and 0.97. The results of AMOVA has revealed 9% of variance among populations and 91% of variance within populations, Φ PT was found as 0.089, which indicates existence of genetic differences though limited. In conclusion, the results indicate that saffron accessions are minimally genetically differentiated, which could be capitalized in future breeding programmes to ameliorate this precious crop.

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Section: Discussionmentioning

confidence: 99%

Deciphering genetic diversity analysis of saffron (Crocus sativus L.) using RAPD and ISSR markers

Mir

Mansoor

Sugapriya

et al. 2021

Saudi Journal of Biological Sciences

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“…Apart from radiotherapy, chemotherapy, immunotherapy and surgery [ 43 ], scientists are also looking for alternative approaches to treat cancer and to improve the life quality of patients. Saffron’s compounds are reported to be a safe and effective treatment to reduce the toxic side effects of some conventional chemotherapeutic drugs such as tamoxifen [ 44 ], cisplatin [ 45 ], and doxorubicin [ 46 ]. Also, besides the protective features mentioned above, several studies have highlighted that the combined treatment of saffron extracts with chemotherapeutic drugs had synergistic effects, enhancing the outcome of the applied treatment.…”

Section: Anticancer Properties Of Saffron and Its Major Ingredientmentioning

confidence: 99%

Recent Advances on the Anticancer Properties of Saffron (Crocus sativus L.) and Its Major Constituents

et al. 2020

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Cancer is the second leading cause of death globally with an estimated 9.6 million deaths in 2018 and a sustained rise in its incidence in both developing and developed countries. According to the WHO, about 1 in 6 deaths is due to cancer. Despite the emergence of many pioneer therapeutic options for patients with cancer, their efficacy is still time-limited and noncurative. Thus, continuous intensive screening for superior and safer drugs is still ongoing and has resulted in the detection of the anticancer properties of several phytochemicals. Among the spices, Crocus sativus L. (saffron) and its main constituents, crocin, crocetin, and safranal, have attracted the interest of the scientific community. Pharmacological experiments have established numerous beneficial properties for this brilliant reddish-orange dye derived from the flowers of a humble crocus family species. Studies in cultured human malignant cell lines and animal models have demonstrated the cancer prevention and antitumor activities of saffron and its main ingredients. This review provides an insight into the advances in research on the anticancer properties of saffron and its components, discussing preclinical data, clinical trials, and patents aiming to improve the pharmacological properties of saffron and its major ingredients.

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“…India’s Kashmir region has been found suitable for the growth and quality supply of KCS with regional agricultural benefit. The KCS and its extract (CSE) has become a popular dietary supplement with bioactive as apocarotenoids (PIC, SAF) and carotenoids (crocins, crocetin) [ 3 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ]. These dietary supplements have been regulated widely and require a validated methodology and data.…”

Section: Discussionmentioning

confidence: 99%

“…Kashmir or Indian Saffron is traditionally used in India as antispasmodic and aphrodisiac, treating dermal diseases, kidney–urinary disorders, and rheumatoid arthritis with widespread cosmetics usage as per folklore, Ayurveda, Siddha, Sowa Rigpa, and Unani medicinal systems [ 1 , 22 , 23 , 24 , 25 , 26 , 27 ]. The recent scientific studies on KCS have shown its potential in AD, cardiac functions, immunomodulatory activity, and anti-tumor agents [ 3 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ]. This increased valuation and consistent growth in the KCS consumption in health benefits have prompted the demand and interest in research as a dietary supplement and nutraceutical.…”

Section: Introductionmentioning

confidence: 99%

Bioanalytical Method Development and Validation Study of Neuroprotective Extract of Kashmiri Saffron Using Ultra-Fast Liquid Chromatography-Tandem Mass Spectrometry (UFLC-MS/MS): In Vivo Pharmacokinetics of Apocarotenoids and Carotenoids

et al. 2021

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Kashmir saffron (Crocus sativus L.), also known as Indian saffron, is an important Asian medicinal plant with protective therapeutic applications in brain health. The main bioactive in Kashmir or Indian Saffron (KCS) and its extract (CSE) are apocarotenoids picrocrocin (PIC) and safranal (SAF) with carotenoids, crocetin esters (crocins), and crocetins. The ultra-fast liquid chromatography(UFLC)- photodiode array standardization confirmed the presence of biomarkers PIC, trans-4-GG-crocin (T4C), trans-3-Gg-crocin (T3C), cis-4-GG-crocin (C4C), trans-2-gg-crocin (T2C), trans-crocetin (TCT), and SAF in CSE. This study’s objectives were to develop and validate a sensitive and rapid UFLC-tandem mass spectrometry method for PIC and SAF along T4C and TCT in rat plasma with internal standards (IS). The calibration curves were linear (R2 > 0.990), with the lower limit of quantification (LLOQ) as 10 ng/mL. The UFLC-MS/MS assay-based precision (RSD, <15%) and accuracy (RE, −11.03–9.96) on analytical quality control (QC) levels were well within the acceptance criteria with excellent recoveries (91.18–106.86%) in plasma samples. The method was applied to investigate the in vivo pharmacokinetic parameters after oral administration of 40 mg/kg CSE in the rats (n = 6). The active metabolite TCT and T4C, PIC, SAF were quantified for the first time with T3C, C4C, T2C by this validated bioanalytical method, which will be useful for preclinical/clinical trials of CSE as a potential neuroprotective dietary supplement.

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Isolation, purification and characterization of naturally derived Crocetin beta-d-glucosyl ester from Crocus sativus L. against breast cancer and its binding chemistry with ER-alpha/HDAC2

Cited by 43 publications

References 44 publications

Deciphering genetic diversity analysis of saffron (Crocus sativus L.) using RAPD and ISSR markers

Deciphering genetic diversity analysis of saffron (Crocus sativus L.) using RAPD and ISSR markers

Recent Advances on the Anticancer Properties of Saffron (Crocus sativus L.) and Its Major Constituents

Bioanalytical Method Development and Validation Study of Neuroprotective Extract of Kashmiri Saffron Using Ultra-Fast Liquid Chromatography-Tandem Mass Spectrometry (UFLC-MS/MS): In Vivo Pharmacokinetics of Apocarotenoids and Carotenoids

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