Isolation, purification and characterization of antioxidative peptide of pearl millet (Pennisetum glaucum) protein hydrolysate

Agrawal, Himani; Joshi, Robin; Gupta, Mahesh

doi:10.1016/j.foodchem.2016.02.127

Cited by 155 publications

(94 citation statements)

References 38 publications

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“…Therefore, finding a scavenging agent for protecting cells from hydroxyl radical‐induced damage is very important. In Table , the hydroxyl radicals (·OH) scavenging activity of QBLH was 82.44 ± 3.35%, whereas pearl millet ( Pennisetum glaucum ) protein hydrolysate exhibited 60.95 ± 0.250% activity on hydroxyl radicals (Agrawal, Joshi, & Gupta, ). Thus, we can suppose that QBLH contained some potent and efficient compounds which could reduce or terminate the radical reaction.…”

Section: Resultsmentioning

confidence: 99%

“…Several previous researches (Agrawal et al, 2016;Wattanasiritham, Theerakulkait, Wickramasekara, Maier, & Stevens, 2016;Xu, Zhao, Qu, & Ye, 2013) demonstrated that high proportion of hydrophobic peptide residues, such as Val, Leu, Ala, Gly, and Pro, exert strong effects on inhibiting oxidant reaction. Consistent with these above reports mentioned, in our study, peak 3 of RP-HPLC actually possessed a large number of hydrophobic amino acids.…”

Section: Amino Acid Components Of the Purified Peptide Versus Antiomentioning

confidence: 99%

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Antioxidant activity of queen bee larvae processed by enzymatic hydrolysis

Deyuan

Dong

Liu

et al. 2017

J Food Process Preserv

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Queen bee larvae (QBL), by‐products of royal jelly processing, was enzymatically hydrolyzed to produce bioactive peptides. Five enzymes were firstly used to obtain hydrolysates which were evaluated for their antioxidative performances. QBL hydrolyzed by pepsin, showing the highest antioxidant resistance, were prepared and isolated by ultrafiltration using different molecular weight cutoffs of <1, 1–5, 5–10, and >10 kDa. Then, hydrolysate III (MW 1–5 kDa) with the highest activity was purified through continuous chromatography. Antioxidant capacities of active peak 3 (P3) to DPPH, hydroxyl, superoxide, ABTS, oxygen radical absorbance capacity radicals assay were 74.31%, 82.44%, 74.53%, 78.81%, and 1,074 μM TE/g, respectively. Finally, the most potent antioxidant hydrolysate was determined through a high‐speed amino acid analyzer. The results of the QBLH for in vitro antioxidant assays have indicated that P3 is a good antioxidative peptide due to its high percentage of hydrophobic amino acids and has vast prospects for future development and application. Practical applications QBL has been used as a traditional medicine for several millennia in China due to its rich nutrition and potent effect in improving human immunity, however, the utilization of QBL resources industry is rather low. This article evaluated the antioxidant activities of queen bee larvae using DPPH, hydroxyl, superoxide, ABTS, ORAC radicals assay, respectively. This article indicates that high antioxidant amino acid residues can be obtained from QBL through simple processing methods (enzymatic hydrolyzing and membrane isolating). Our study could arouse a great interest among researchers to develop a new series of QBL products with high efficient antioxidant properties.

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Section: Resultsmentioning

confidence: 99%

Section: Amino Acid Components Of the Purified Peptide Versus Antiomentioning

confidence: 99%

Antioxidant activity of queen bee larvae processed by enzymatic hydrolysis

Deyuan

Dong

Liu

et al. 2017

J Food Process Preserv

View full text Add to dashboard Cite

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“…Food-derived antioxidant peptides are attracting the interest of research globally because of their safety (Papas, 1999). A number of antioxidant peptides from various animal and plant protein hydrolysates, such as Chinese chestnut protein (Feng, Ruan, Jin, Xu, & Wang, 2018), walnut protein (Liu et al, 2016), pearl millet protein (Agrawal, Joshi, & Gupta, 2016), and monkfish muscle (Chi et al, 2014), have been reported recently.…”

Section: Introductionmentioning

confidence: 99%

A possible mechanism for enhancing the antioxidant activity by pulsed electric field on pine nut peptide Glutamine‐Tryptophan‐Phenylalanine‐Histidine

Zhang

Xing

et al. 2018

J Food Biochem

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The aim of this study was to investigate the possible mechanism for increasing the antioxidant activity on peptide Glutamine‐Tryptophan‐Phenylalanine‐Histidine (QWFH) from pine nut (Pinus koraiensis) protein by a pulsed electric field (PEF). The antioxidant capacity of PEF‐treated QWFH increased significantly (p < 0.05) through 1,1‐diphenyl‐2‐pycryl‐hydrazyl radical scavenging and oxygen radical absorbance capacity assays. A series of mechanism exploration methods, including reversed‐phase high‐performance liquid chromatography, ultraviolet absorption spectroscopy, intrinsic fluorescence spectra, circular dichroism spectroscopy, and 1D and 2D nuclear magnetic resonance spectroscopies, were applied. QWFH chain was not cleaved by the PEF treatment, while more aromatic amino acids (Trp and Phe) were exposed to the polar solvent. In addition, the content of random coil of QWFH in solution was increased and its active hydrogen was changed after the PEF treatment. Moreover, the long‐range connectivity between OH (14.234 ppm) on 4‐H His, NαH (7.295 ppm) on 3‐H Phe, and NαH2 (6.801 ppm) on 1‐H Gln disappeared due to the PEF. Practical applications Antioxidants have been extensively explored as a potential drug to decrease the risk of certain chronic diseases. Food‐derived bioactive compounds are safer than synthetic antioxidants for human health and well‐being. And the PEF technology is one of the promising processes for improving the biological activity of food components. Currently, the activity of the antioxidant peptide QWFH increased after a PEF treatment. The basic structure of QWFH did not change, but the unfolding of the secondary structure on the peptide chain and the displacement of the active hydrogen increased the antioxidant activity of the peptide. Thus, the range of application of a PEF has been expanded and it also benefited the development of more functional factors in the functional food industry.

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“…Compared with the animal drugs from TCM, the research of TCM proteins from herbal medicine seems to be ignored. Instead, plenty of oligopeptides and peptide fractions have been identified from non-medicinal plants, such as Sorghum bicolor [3], Pennisetum glaucum [4], and so on. However, the studies on the bioactivities of these plant peptides are often random, due to the lack of traditional pharmacological effect like TCM.…”

Section: Introductionmentioning

confidence: 99%

Discovery of Anti-Hypertensive Oligopeptides from Adlay Based on In Silico Proteolysis and Virtual Screening

Qiao

Chen

et al. 2016

IJMS

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Adlay (Coix larchryma-jobi L.) was the commonly used Traditional Chinese Medicine (TCM) with high content of seed storage protein. The hydrolyzed bioactive oligopeptides of adlay have been proven to be anti-hypertensive effective components. However, the structures and anti-hypertensive mechanism of bioactive oligopeptides from adlay were not clear. To discover the definite anti-hypertensive oligopeptides from adlay, in silico proteolysis and virtual screening were implemented to obtain potential oligopeptides, which were further identified by biochemistry assay and molecular dynamics simulation. In this paper, ten sequences of adlay prolamins were collected and in silico hydrolyzed to construct the oligopeptide library with 134 oligopeptides. This library was reverse screened by anti-hypertensive pharmacophore database, which was constructed by our research team and contained ten anti-hypertensive targets. Angiotensin-I converting enzyme (ACE) was identified as the main potential target for the anti-hypertensive activity of adlay oligopeptides. Three crystal structures of ACE were utilized for docking studies and 19 oligopeptides were finally identified with potential ACE inhibitory activity. According to mapping features and evaluation indexes of pharmacophore and docking, three oligopeptides were selected for biochemistry assay. An oligopeptide sequence, NPATY (IC50 = 61.88 ± 2.77 µM), was identified as the ACE inhibitor by reverse-phase high performance liquid chromatography (RP-HPLC) assay. Molecular dynamics simulation of NPATY was further utilized to analyze interactive bonds and key residues. ALA354 was identified as a key residue of ACE inhibitors. Hydrophobic effect of VAL518 and electrostatic effects of HIS383, HIS387, HIS513 and Zn2+ were also regarded as playing a key role in inhibiting ACE activities. This study provides a research strategy to explore the pharmacological mechanism of Traditional Chinese Medicine (TCM) proteins based on in silico proteolysis and virtual screening, which could be beneficial to reveal the pharmacological action of TCM proteins and provide new lead compounds for peptides-based drug design.

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Isolation, purification and characterization of antioxidative peptide of pearl millet (Pennisetum glaucum) protein hydrolysate

Cited by 155 publications

References 38 publications

Antioxidant activity of queen bee larvae processed by enzymatic hydrolysis

Antioxidant activity of queen bee larvae processed by enzymatic hydrolysis

A possible mechanism for enhancing the antioxidant activity by pulsed electric field on pine nut peptide Glutamine‐Tryptophan‐Phenylalanine‐Histidine

Discovery of Anti-Hypertensive Oligopeptides from Adlay Based on In Silico Proteolysis and Virtual Screening

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