Isolation, proliferation and characterization of endometrial canine stem cells

Abstract

In the last decade, progenitor cells isolated from dissociated endometrial tissue have been the subject of many studies in several animal species. Recently, endometrial cells showing characteristics of mesenchymal stem cells (MSC) have been demonstrated in human, pig and cow uterine tissue samples. The aim of this study was the isolation and characterization of stromal cells from the endometrium of healthy bitches, a tissue that after elective surgery is routinely discarded. Multipotent stromal cells could be isolated from all bitches enrolled in the study (n = 7). The multipotency of cells was demonstrated by their capacity to differentiate into adipocytic, osteocytic and chondrocytic lineages. Clonogenicity and cell proliferation ability were also tested. Furthermore, gene expression analysis by RT-PCR was used to compare the expression of a set of genes (CD44, CD29, CD34, CD45, CD90, CD13, CD133, CD73, CD31 CD105, Oct4) with adipose tissue-derived MSC. Stromal cells isolated from uterine endometrium showed similar morphology, ability of subculture and plasticity, and also expressed a panel of genes comparable with adipose tissue-derived MSC. These data suggest that endometrial stromal cells fulfil the basic criteria proposed by the "Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy" for the identification of mesenchymal stem cells. Although endometrial mesenchymal stem cells (EnMSC) showed a lower replicative ability in comparison with adipose tissue-derived MSC, they could be considered a cell therapeutic agent alternative to adipose tissue or bone marrow-derived MSC in dog.

Mesenchymal stem cell basic research and applications in dog medicine

“…Infrapatellar fat pad‐derived dMSCs though carry comparable proliferation potential to that of the synovium but the chondrogenesis may be better in case of Sy‐MSCs (Sasaki et al, ). Between dEn‐MSCs and dAD‐MSCs, former cells carry lower replicative potential as compared with latter (De Cesaris et al, ). dBM‐MSCs and dLi‐MSCs though carry similar proliferation potential but differ in their differentiation potential.…”

Isolation and characterization mesenchymal stem cells from red panda (Ailurus fulgens styani) endometrium

“…Moreover, eMSCs could generate endometrial stroma in xenograft assays ( Masuda et al, 2010 ). eMSCs have been harvested and characterized from humans ( Cheng et al, 2017 ; Queckborner et al, 2020 ), pigs ( Miernik & Karasinski, 2012 ), heifers ( Calle et al, 2019 ; de Moraes et al, 2016 ), horses ( Cabezas et al, 2018 ), dogs ( De Cesaris et al, 2017 ; Sahoo et al, 2017 ) and sheep ( Ghobadi et al, 2018 ). This type of cells showed similar properties to bone marrow MSCs and may provide an available source for cell-based therapies due to their strong regenerative capacity ( Gargett et al, 2009 ).…”

Isolation, culture, characterization, and osteogenic differentiation of canine endometrial mesenchymal stem cell

“…The multipotent stem cells (MSCs) were first isolated and characterized from bone marrow [ 5 ] and later on from various tissues such as umbilical cord blood [ 6 ], adipose tissue [ 7 ], amniotic fluid [ 8 ], amniotic membrane [ 9 ], and peripheral blood [ 10 ]. The existence of endometrial mesenchymal stem cell (En-MSCs) population in different species has been demonstrated by different groups in heifer [ 11 ], human [ 12 ], murine [ 13 ], ovine [ 14 ], equine [ 15 ], and canine [ 16 ].…”