Isolation, partial amino acid sequence, and immunohistochemical localization of a brain-specific calcium-binding protein.

Winsky, Lois; Nakata, Hajime; Martin, Brian M.; Jacobowitz, David M.

doi:10.1073/pnas.86.24.10139

Cited by 155 publications

(75 citation statements)

References 20 publications

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“…The calbindin-D 28k antibody is derived from CB-955 hybridomas produced by fusion of mouse myeloma cells and splenocytes from BALB/c mice that were immunized with purified bovine kidney calbindin-D 28k . The specificity of this antibody has been demonstrated through preadsorption immunohistochemical assays that abolish calbindin labeling (Celio, 1990), through Western blot analysis of rat brain tissue which shows a distinct band at 28kD (Miyata et al, 2000) and through immunohistochemistry which shows calbindin immunostaining in brain regions known to express a significant level of calbindin-D 28k mRNA Miyata et al, 2000;Winsky et al, 1989).…”

Section: Primary Antibodiesmentioning

confidence: 99%

Subcellular and subsynaptic localization of group I metabotropic glutamate receptors in the nucleus accumbens of cocaine-treated rats

2008

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There is significant pharmacological and behavioral evidence that group I metabotropic glutamate receptors (mGluR1a and mGluR5) in the nucleus accumbens play an important role in the neurochemical and pathophysiological mechanisms that underlie addiction to psychostimulants. To further address this issue, we undertook a detailed ultrastructural analysis to characterize changes in the subcellular and subsynaptic localization of mGluR1a and mGluR5 in the core and shell of nucleus accumbens following acute or chronic cocaine administration in rats. After a single cocaine injection (30mg/kg) and 45 minutes withdrawal, there was a significant decrease in the proportion of plasma membrane-bound mGluR1a in accumbens shell dendrites. Similarly, the proportion of plasma membrane-bound mGluR1a was decreased in large dendrites of accumbens core neurons following chronic cocaine exposure (i.e. 1 week treatment followed by three weeks withdrawal). However, neither acute nor chronic cocaine treatments induced significant change in the localization of mGluR5 in accumbens core and shell, which is in contrast with the significant reduction of plasma membranebound mGluR1a and mGluR5 induced by local intra-accumbens administration of the group I mGluR agonist, DHPG. In conclusion, these findings demonstrate that cocaine-induced glutamate imbalance (Smith et al., 1995;Pierce et al., 1996;Reid et al., 1997) has modest effects on the trafficking of group I mGluRs in the nucleus accumbens. These results provide valuable information on the neuroadaptive mechanisms of accumbens group I mGluRs in response to cocaine administration.

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Section: Primary Antibodiesmentioning

confidence: 99%

Subcellular and subsynaptic localization of group I metabotropic glutamate receptors in the nucleus accumbens of cocaine-treated rats

2008

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“…We used the anti-calretinin antibody (Winsky et al, 1989) to visualize the olfactory sensory neurons. This anti- body appears to recognize both microvillar and ciliated olfactory receptors in trout (Porteros et al, 1997) and the pattern of expression we observed is similar to that reported in embryos expressing green fluorescent protein (GFP) under the Olfactory Marker Protein (OMP) promoter (Yoshida and Mishina, 2003).…”

Section: Morphological Defects Of the Lre Mutantmentioning

confidence: 99%

Isolation and characterization of the laure olfactory behavioral mutant in the zebrafish, Danio rerio

Vitebsky

Reyes

Sanderson

et al. 2005

Developmental Dynamics

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To initiate a genetic analysis of olfactory development and function in the zebrafish, Danio rerio, we developed a behavioral genetic screen for mutations affecting the olfactory sensory system. First, we characterized olfactory responses of wild-type zebrafish to various odors. We found that 3-day-old juvenile zebrafish reacted to the amino acid L-cysteine with an aversive behavioral response. We isolated one mutant, laure (lre), which showed no aversive behavioral response to L-cysteine at 3 days of development, and carried out a preliminary characterization of this mutant's defects. We found that lre mutant fish were also defective in their response to L-serine and L-alanine, but not to taurocholic acid, as young adults. In addition, lre mutant fish had significantly fewer primary olfactory sensory neurons than normal, and the axons of these neurons did not form the characteristic axon termination pattern in the developing olfactory bulb. Nevertheless, the olfactory epithelium of lre mutant fish showed normal or near normal electrophysiological responses to several odorants. Our data suggest that the behavioral defects observed in the lre mutant result from the disruption of the developing olfactory sensory neurons and their axonal connections within the olfactory bulb. The isolation of the lre mutant shows that our behavior-based screen represents a viable approach for carrying out a genetic dissection of olfactory behaviors in this vertebrate model system. Developmental Dynamics 234:229 -242, 2005.

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“…61(3): 267-269, 1999 incubated with polyclonal rabbit antibodies (IgG) against guinea-pig calretinin (diluted 1:5,000; AB149; Chemicon, Temecula, CA, U.S.A.) for 72 hr at 4°C. The specificity of the calretinin antibody has been described previously [11]. After incubation, the specimens were treated with biotinylated antibodies that had been raised in goat against rabbit IgG (Vector, Burlingame, CA, U.S.A.) and reagents from an ABC kit (Elite ABC kit; Vector) for 90 min each at room temperature.…”

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confidence: 99%

Calretinin Immunoreactive Nerve Endings in the Trachea and Bronchi of the Rat.

Yamamoto

Atoji

Suzuki

1999

The Journal of Veterinary Medical Science

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ABSTRACT. Nerve endings showing calretinin immunoreactivity were examined in the lower respiratory tract of the adult rat. Tree-like nerve endings were immunostained in the tracheal and bronchial smooth muscle layer. The endings that arose from thick nerve fibers and formed corpuscles composed of many arborized nerve terminals. A few of the nerve endings were also observed in the lamina propria of the tracheal mucosa, close to the epithelial layer. Immunoelectron microscopy revealed that the immunoreactive terminals were filled with mitochondria and scattered among the intermuscular collagen fibrils. Schwann cell sheath and collagen fibrils were intercalated between the smooth muscle cells and nerve endings. The calretinin immunoreactive nerve endings observed in the present study seem to be slowly adapting stretch receptors.-KEY WORDS: calretinin, sensory nerve ending, trachea.J. Vet. Med. Sci. 61(3): 267-269, 1999 incubated with polyclonal rabbit antibodies (IgG) against guinea-pig calretinin (diluted 1:5,000; AB149; Chemicon, Temecula, CA, U.S.A.) for 72 hr at 4°C. The specificity of the calretinin antibody has been described previously [11]. After incubation, the specimens were treated with biotinylated antibodies that had been raised in goat against rabbit IgG (Vector, Burlingame, CA, U.S.A.) and reagents from an ABC kit (Elite ABC kit; Vector) for 90 min each at room temperature. Immunoreactions were made visible by incubation with Tris-HCl buffer containing 3,3'-diaminobenzidine tetrahydrochloride and H 2 O 2 . Immunostained specimens were mounted on gelatin-coated slides, air-dried, dehydrated in ethanol, cleared with xylene, and sealed with coverslips. For examination by immunoelectron microscopy, 50-µm-thick cryostat sections of the thoracic trachea and the lung were immunostained with calretinin antibodies without treatment with PBS containing Triton X-100 or H 2 O 2 . Portions of the sections containing immunostained endings were removed under the dissecting microscope, postfixed in 1% osmium tetroxide for 60 min, dehydrated with a graded ethanol series, and embedded in Epon 812. The ultrathin sections, without any contrast staining, were examined by a transmission electron microscope at 75 kV (H-8100; Hitachi, Tokyo, Japan).Tree-like nerve endings showing calretinin immunoreactivity were identified in the smooth muscle layer of the trachea and the larger bronchi (Fig. 1A-C). The endings were distributed mainly in the smooth muscle layer of the thoracic trachea and the principal and lobar bronchi, without the smaller bronchi. Only 1 to 10 endings were found in the whole length of the trachea (Fig. 2). A few endings were also observed in the lamina propria of the tracheal mucosa close to the epithelial layer (Fig. 1D).Thick nerve fibers with or without swellings ramified into several branches to form the tree-like endings (Fig. 1). The axons ended with laminar terminal arborizations. The corpuscles were arranged in parallel to the longitudinal axis of the smooth muscle cells, about 100-200 µm in le...

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Isolation, partial amino acid sequence, and immunohistochemical localization of a brain-specific calcium-binding protein.

Cited by 155 publications

References 20 publications

Subcellular and subsynaptic localization of group I metabotropic glutamate receptors in the nucleus accumbens of cocaine-treated rats

Subcellular and subsynaptic localization of group I metabotropic glutamate receptors in the nucleus accumbens of cocaine-treated rats

Isolation and characterization of the laure olfactory behavioral mutant in the zebrafish, Danio rerio

Calretinin Immunoreactive Nerve Endings in the Trachea and Bronchi of the Rat.

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