Isolation, Optimization, and Structural Characterization of Glycolipid Biosurfactant Produced by Marine Isolate Shewanella algae B12 and Evaluation of Its Antimicrobial and Anti-biofilm Activity

Gharaei, S. Khajoei; Ohadi, Mandana; Hassanshahian, Mehdi; Porsheikhali, Sara; Forootanfar, Hamid

doi:10.1007/s12010-021-03782-8

Cited by 28 publications

(17 citation statements)

References 48 publications

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“…Oil-degrading Shewanella cultures were also isolated from oil reservoirs [ 78 , 79 ] and seawater of the Persian Gulf [ 80 , 81 ]. The formation of glycolipid biosurfactants by a marine isolate Shewanella algae B12 has been shown [ 82 ]. Members of the genus Shewanella are able to utilize a range of organic substrates, to reduce iron and a number of other metals, metalloids, and radionuclides [ 83 , 84 ]; their presence in a sample of littoral soil 3_M21 may be a consequence of metal contamination of coastal areas and seawater, noted earlier ( (accessed on 21 June 2022)).…”

Section: Resultsmentioning

confidence: 99%

Microbial Communities of Seawater and Coastal Soil of Russian Arctic Region and Their Potential for Bioremediation from Hydrocarbon Pollutants

et al. 2022

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The development of Arctic regions leads to pollution of marine and coastal environments with oil and petroleum products. The purpose of this work was to determine the diversity of microbial communities in seawater, as well as in littoral and coastal soil, and the potential ability of their members to degrade hydrocarbons degradation and to isolate oil-degrading bacteria. Using high-throughput sequencing of the V4 region of the 16S rRNA gene, the dominance of bacteria in polar communities was shown, the proportion of archaea did not exceed 2% (of the total number of sequences in the libraries). Archaea inhabiting the seawater belonged to the genera Nitrosopumilus and Nitrosoarchaeum and to the Nitrososphaeraceae family. In the polluted samples, members of the Gammaproteobacteria, Alphaproteobacteria, and Actinomycetes classes predominated; bacteria of the classes Bacteroidia, Clostridia, Acidimicrobiia, Planctomycetia, and Deltaproteobacteria were less represented. Using the iVikodak program and KEGG database, the potential functional characteristics of the studied prokaryotic communities were predicted. Bacteria were potentially involved in nitrogen and sulfur cycles, in degradation of benzoate, terephthalate, fatty acids, and alkanes. A total of 19 strains of bacteria of the genera Pseudomonas, Aeromonas, Oceanisphaera, Shewanella, Paeniglutamicibacter, and Rhodococcus were isolated from the studied samples. Among them were psychrotolerant and psychrophilic bacteria growing in seawater and utilizing crude oil, diesel fuel, and motor oils. The data obtained suggest that the studied microbial communities could participate in the removal of hydrocarbons from arctic seawater and coastal soils and suggested the possibility of the application of the isolates for the bioaugmentation of oil-contaminated polar environments.

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Section: Resultsmentioning

confidence: 99%

Microbial Communities of Seawater and Coastal Soil of Russian Arctic Region and Their Potential for Bioremediation from Hydrocarbon Pollutants

et al. 2022

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“…The antimicrobial effects of biosurfactants against various microorganisms have been investigated by numerous studies. For instance, S. gharaei (2022) showed the glycolipid biosurfactant produced by marine bacteria renders signi cant antimicrobial activity versus a wide range of pathogenic bacteria [19].…”

Section: Agar Diffusion Methodsmentioning

confidence: 99%

“…The PCR program involved an initial template denaturation step at 94 ℃ for 1 minute, followed by 30 cycles of denaturation at 94 ℃ for 60 seconds, annealing at 55 ℃ for 1 minute, synthesis at 72 ℃ for 2 minutes, and nal extension at 72 ℃ for 2 minutes. Subsequently, the PCR product was separated on a 1% agarose gel, subjected to sequencing, and compared to available sequences in the GeneBank using the basic local alignment search tool (BLAST) [19].…”

Section: Molecular Identi Cation Of Bacterial Strainmentioning

confidence: 99%

Structural characterization of biosurfactant produced by marine bacterium Pseudomonas fragi strain F1 (isolated from Persian Gulf) and evaluation of antimicrobial and antibiofilm activity

Amirinejad,

Shekarchizadeh,

Mousavi

et al. 2023

Preprint

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The marine environment is a rich source of bioactive compounds, including biosurfactant-producing bacteria that exhibit unique characteristics and functionalities. In this study, we focused on glycolipid biosurfactants produced bacteria in a commensal relationship with marine organisms. We isolated and characterized a biosurfactant-producing strain, Pseudomonas fragi strain F1, which displayed high hemolytic activity (27mm), oil spreading ability (4mm), emulsification index (40%), and decreasing surface tension (31.3 mN/m). Fourier transform infrared (FT-IR) analysis revealed the glycolipid nature of the produced biosurfactant. Elemental analysis using CHNS and Energy Dispersive Spectroscopy (EDS) confirmed the presence of carbon, hydrogen, nitrogen, sulfur, chlorine, potassium, oxygen, and some other elements in the biosurfactant. The critical micelle concentration (CMC) of the biosurfactant was measured at 350 mg.L− 1, indicating its high efficiency. Furthermore, the biosurfactant demonstrated significant antimicrobial activity against Gram-positive and Gram-negative bacteria (the largest obtained ZOI was associated to P. aeruginosa with 27 mm), making it a potential alternative to synthetic drugs. The biosurfactant also exhibited substantial inhibition of biofilm formation and disruption, as well as enzymatic activity reduction in treated bacteria. Moreover, the mixture of biosurfactant and F1 bacterium enhanced the degradation of crude oil (86%), indicating its potential for environmental remediation. These findings highlight the importance of exploring commensal biosurfactant-producing strains in marine environments for hydrocarbon degradation, combating antibiotic resistance, and disrupting microbial biofilms.

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“…The bacterial genera Pseudomonas and Bacillus are the ones that are utilized the most frequently in biosurfactant manufacturing. A significant number of currently available publications have demonstrated their efficacy in biosurfactant production and their role in successfully managing phytopathogens [5,117–119] . Varnier et al., [120] concluded that Pseudomonas aeruginosa produced Rhamnolipid biosurfactants that improved grapevine immunity against Botrytis cinerea .…”

Section: Application Of Biosurfactantmentioning

confidence: 99%

“…A significant number of currently available publications have demonstrated their efficacy in biosurfactant production and their role in successfully managing phytopathogens. [5,[117][118][119] Varnier et al, [120] concluded that Pseudomonas aeruginosa produced Rhamnolipid biosurfactants that improved grapevine immunity against Botrytis cinerea. In addition, using surfactants prevents the virus's spores from germinating and slows the growth of the mycelium they produce.…”

Section: Biosurfactants As Antimicrobial Against Plant Diseasesmentioning

confidence: 99%

Prospects of Microbial Bio‐surfactants To Endorse Prolonged Conservation in the Pharmaceutical and Agriculture Industries

Karnwal

2023

ChemistrySelect

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Chemical surfactants have raised concerns due to their negative impact on ecosystems, prompting the search for ecofriendly alternatives. Biosurfactants derived from microorganisms offer a promising solution. These biomolecules are less harmful and can replace toxic pesticide surfactants. Biosurfactants have shown potential in agriculture for food digestion, crop protection, soil fertility, disease management, antibacterial properties, and biofilm prevention. They eliminate plantpathogens and improve nutrient availability, supporting plantgrowth. Rhamnolipids, produced by Pseudomonas, reduce surface tension, are used as wetting agents and emulsifiers in agriculture, and antimicrobial and anti-inflammatory medications. Surfactin, a biosurfactant from Bacillus subtilis, improves plant development, controls plant diseases, and fights pathogens and diseases. Candida bombicola yeast produces sopho-rolipids, biodegradable surfactants with antibacterial, anticancer, and anti-inflammatory properties. Pseudozyma yeast produces mannosylerythritol lipids (MELs), which biocontrol fungus and have antibacterial, anticancer, and immunomodulatory activities. Biosurfactants also hold potential in pharmaceuticals, functioning as antioxidants, exhibiting antibacterial and anticancer activities, and acting as drug-delivery systems. However, challenges in biosurfactant production include varying research methods, limited production organism sources, and cost implications for large-scale manufacturing. This minireview explores microbiologically produced biosurfactants, their regulatory parameters, their applications in optimizing soil health and controlling plant infections, and their potential roles in the pharmaceutical sector.* Ability to tolerate high temperatures, * Ability to tolerate variation in pH, * Ability to tolerate saline environment, * Biodegradable character, * Low or non-toxic nature, and * Effective selectivity.Generally, biosurfactants are amphipathic molecules characterized by hydrophobic and hydrophilic components. Generally, the hydrophobic component consists of a very long chain of fatty acids connected. The hydrophilic component comprises positively, negatively, or amphoterically charged ions. In contrast to their chemical analogs, biosurfactants are often categorized by their CMC concentration, low or high molecular weight, the microorganism type that produce biosurfactant, and their mode of action. Low molecular weight biosurfactants are most frequently reported as glycolipids, phospholipids, and [a]

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Isolation, Optimization, and Structural Characterization of Glycolipid Biosurfactant Produced by Marine Isolate Shewanella algae B12 and Evaluation of Its Antimicrobial and Anti-biofilm Activity

Cited by 28 publications

References 48 publications

Microbial Communities of Seawater and Coastal Soil of Russian Arctic Region and Their Potential for Bioremediation from Hydrocarbon Pollutants

Microbial Communities of Seawater and Coastal Soil of Russian Arctic Region and Their Potential for Bioremediation from Hydrocarbon Pollutants

Structural characterization of biosurfactant produced by marine bacterium Pseudomonas fragi strain F1 (isolated from Persian Gulf) and evaluation of antimicrobial and antibiofilm activity

Prospects of Microbial Bio‐surfactants To Endorse Prolonged Conservation in the Pharmaceutical and Agriculture Industries

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