Isolation, optimization, and purification of extracellular levansucrase from nonpathogenic Klebsiella strain L1 isolated from waste sugarcane bagasse

Desai, Maitri; Patel, Kartik

doi:10.1016/j.bcab.2019.101107

Cited by 18 publications

(8 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Hitherto, more than one hundred bacterial genera were described as levan producers [ 2 , 8 ]. Levansucrase is secreted by microorganisms into the extracellular environment particularly at low and neutral pH [ 9 ]. This enzyme works efficiently when sucrose is used as a sole carbon source and displays low activity with other types of sugars such as raffinose, mannose, fructose, glucose, and others [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Bioactive Levan-Type Exopolysaccharide Produced by Pantoea agglomerans ZMR7: Characterization and Optimization for Enhanced Production

Al-Qaysi

Al-Haideri

AL-Shimmary

et al. 2021

J. Microbiol. Biotechnol.

View full text Add to dashboard Cite

Levan is an industrially important, functional biopolymer with considerable applications in the food and pharmaceutical fields owing to its safety and biocompatibility. Here, levan-type exopolysaccharide produced by Pantoea agglomerans ZMR7 was purified by cold ethanol precipitation and characterized using TLC, FTIR, 1 H, and 13 C NMR spectroscopy. The maximum production of levan (28.4 g/l) was achieved when sucrose and ammonium chloride were used as carbon and nitrogen sources, respectively, at 35°C and an initial pH of 8.0. Some biomedical applications of levan like antitumor, antiparasitic, and antioxidant activities were investigated in vitro. The results revealed the ability of levan at different concentrations to decrease the viability of rhabdomyosarcoma and breast cancer cells compared with untreated cancer cells. Levan appeared also to have high antiparasitic activity against the promastigote of Leishmania tropica. Furthermore, levan had strong DPPH radical scavenging (antioxidant) activity. These findings suggest that levan produced by P. agglomerans ZMR7 can serve as a natural biopolymer candidate for the pharmaceutical and medical fields.

show abstract

Section: Introductionmentioning

confidence: 99%

Bioactive Levan-Type Exopolysaccharide Produced by Pantoea agglomerans ZMR7: Characterization and Optimization for Enhanced Production

Al-Qaysi

Al-Haideri

AL-Shimmary

et al. 2021

J. Microbiol. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…At pH less than 6.0, the enzyme catalyzes almost exclusively the synthesis of low molecular weight levan in its microfibril form. Additionally, studies on Brenneria goodwinii [ 16 ], Bacillus subtilis [ 38 , 45 ], Bacillus subtilis NATTO [ 27 ], Bacillus methylotrophicus [ 50 ], Klebsiella species [ 20 ], Lactobacillus reuteri [ 51 ], and Zymomonas mobilis [ 14 ] have demonstrated that a pH range of 5.0–6.5 is optimal for levansucrase activity. However, pH extremes have a significant negative effect on levansucrase activity.…”

Section: Discussionmentioning

confidence: 99%

“…It is synthesized by the levansucrase enzyme (EC 2.4.1.10), a fructosyltransferase belonging to the glycoside hydrolase family 68 (GH68). This GH68 is found to be produced by several bacterial strains [ 15 ], including the species Brenneria goodwini [ 16 ], Bacillus subtilis [ 17 ], Gluconobacter japonicus [ 18 ], Halomonas species [ 19 ], Klebsiella strain [ 20 ], Leuconostoc citreum [ 21 ], and Zymomonas mobilis [ 22 ]. Levansucrase functions by catalyzing the conversion of sucrose to levan via the fructosyltransferase reaction.…”

Section: Introductionmentioning

confidence: 99%

Characterization of levansucrase produced by novel Bacillus siamensis and optimization of culture condition for levan biosynthesis

Phengnoi

Thakham

Rachphirom

et al. 2022

Heliyon

View full text Add to dashboard Cite

“…Amplification of the target DNA was performed in a volume of 50 µL by mixing 20 ng of the template DNA with 2.5 mM concentration of dNTPs, 1 mM concentration of each universal primer (reverse-1492r and forward-27f), and 3U of Taq DNA polymerase in 10X Taq buffer (Thermo Fisher Scientific, Bengaluru, India). The amplification procedure was performed using a GeneAmp PCR system 9700 (Applied Biosystems, Waltham, MA, USA) with the conditions described by Desai and Patel (2019) [57]. As part of the Sanger sequencing process, a purified PCR amplicon was sequenced at Eurofins Genomic India Pvt Ltd., Bangalore, India.…”

Section: Identification Of Potent Bacterial Strain Dg-20mentioning

confidence: 99%

Cadmium-Tolerant Plant Growth-Promoting Bacteria Curtobacterium oceanosedimentum Improves Growth Attributes and Strengthens Antioxidant System in Chili (Capsicum frutescens)

Patel

Al-Keridis

et al. 2022

Sustainability

Self Cite

View full text Add to dashboard Cite

The remediation of potentially toxic element-polluted soils can be accomplished through the use of microbial and plant-assisted bioremediation. A total of 32 bacteria were isolated from soil samples contaminated with potentially toxic elements. The isolated bacterial strain DG-20 showed high tolerance to cadmium (up to 18 mM) and also showed bioaccumulative Cd removal properties, as demonstrated by atomic absorption spectroscopy studies. By sequencing the 16S rRNA gene, this strain was identified as Curtobacterium oceanosedimentum. Under stress and normal conditions, isolate DG-20 also produced a wide range of plant growth promoting traits, including ammonia production (51–73 µg/mL) and IAA production (116–183 µg/mL), alongside siderophore production and phosphate solubilization. Additionally, pot experiments were conducted to determine whether the strain could promote Chili growth when Cd salts are present. Over the control, bacterial colonization increased root and shoot lengths significantly up to 58% and 60%, respectively. Following inoculation with the Cd-tolerant strain, the plants also increased in both fresh and dry weight. In both the control and inoculated plants, Cd was accumulated more in roots than in shoots, indicating that Chili was phytostabilizing Cd levels. Besides improving the plant attributes, Cd-tolerant bacteria were also found to increase the amount of total chlorophyll, proline, total phenol, and ascorbic acid in the soil when added to the soil. These results suggest that the inoculant provides protection to plants from negative effects. The results of the present study predict that the combined properties of the tested strain in terms of Cd tolerance and plant growth promotion can be exploited for the purpose of the bioremediation of Cd, and for the improvement of Chili cultivation in soils contaminated with Cd.

show abstract

Isolation, optimization, and purification of extracellular levansucrase from nonpathogenic Klebsiella strain L1 isolated from waste sugarcane bagasse

Cited by 18 publications

References 28 publications

Bioactive Levan-Type Exopolysaccharide Produced by Pantoea agglomerans ZMR7: Characterization and Optimization for Enhanced Production

Bioactive Levan-Type Exopolysaccharide Produced by Pantoea agglomerans ZMR7: Characterization and Optimization for Enhanced Production

Characterization of levansucrase produced by novel Bacillus siamensis and optimization of culture condition for levan biosynthesis

Cadmium-Tolerant Plant Growth-Promoting Bacteria Curtobacterium oceanosedimentum Improves Growth Attributes and Strengthens Antioxidant System in Chili (Capsicum frutescens)

Contact Info

Product

Resources

About