Isolation of Yersinia enterocolitica from Pig Tonsils

Abstract

Yersinia enterocolitica serotype 0:5 was isolated from the tonsils of 8 of 27 butcher hogs subjected to normal slaughtering procedures. Isolation through enrichment in cold mannitol broth or a bile medium at 4 C for 10–60 days was equally effective. Enrichment in modified Rappaport broth at 25 C for longer than 2 days sharply decreased recovery of Y. enterocolitica from tonsils. The type of enrichment medium also had a marked effect on the recovery efficiency of different strains of Y. enterocolitica from ground meat.

Yersinia Enterocolitica and Yesinia Enterocolitica‐like Species: Their Pathogenicity and Significance in Foods

“…Warm-blooded animals probably constitute the largest reservoir of Y. enterocolitica (Hubbert 1972;Kapperud 1975;Mollaret 1971 (Black et al 1978) and swine (Hanna et al 1980). Doyleet al (1981) have isolated serotype 0:s strains, as well as other serotypes, from porcine tongues.…”

“…Y. enterocolitica, Y. frederiksenii and Y. intermedia are consistently isolated from pork, beef and lamb (Leistner et al 1975;Asakawa et al 1979; Inoue and Kurose 1975;Hanna et al 1976). Y. enterocolitica has been most frequently isolated from swine carcasses and pork (Toma and Dedrick 1975;Leistner et al 1975;Zen-Yoji et al 1974;Esseveld and Goudzwaard 1973;Tsubokura et al 1973;Wauters and Janssens 1976;Hanna et al 1980;Doyle et al 1981;Schiemann and Fleming 1981). Clearly, swine serve as a natural reservoir for Y. enterocolitica; however, there are insufficient data to determine whether or not swine are a major source of human infection.…”

Prevalence of Yersinia enterocolitica across phases of swine production

“…(Anonymous, 1990;Anonymous, 2003) Of particular food safety concern is the ability for Y. enterocolitica to grow at refrigeration temperatures and survive repeated freezing and thawing. (Toora et al, 1992) Previous studies of Y. enterocolitica have investigated swine at harvest (Hanna et al, 1980;Harmon et al, 1984) but little research has focused on the epidemiology of Y. enterocolitica at the farm level. Understanding the on-farm epidemiology of Y. enterocolitica is the first step towards identifying risk factors and potential interventions in swine production that may decrease the risk of product contamination during harvest and processing.…”

Effects of irradiation on selected pathogens in meat and meat products

“…This organism has been isolated from many locations on pigs, but mostly from the oral cavity (Nesbakken, 1988), including the tongue (Doyle, 1981), throat (Schiemann and Fleming, 1981), and tonsils (Hanna et al, 1980). Enterotoxigenic strains were found in minced meat (Kleinlein and Untermann, 1990), various retail pork products (Schiemann, 1980), and vacuum-packages of beef and lamb ).…”