Isolation of Yersinia enterocolitica Bacteriophage From Hospital Wastewater

Daneshgar, Fatemeh; Dallal, Mohammad Mehdi Soltan; Hosseini, Farzaneh

doi:10.15171/ijep.2018.05

Cited by 6 publications

(3 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Researchers isolated bacteriophages from raw hospital wastewater using the food-borne bacteria Yersinia enterocolitica ( Daneshgar et al, 2018 ). The phages were active at temperatures ranging between 4 and 70 °C; however, the phages became inactive at 80 °C.…”

Section: Sources Of Bacteriophagesmentioning

confidence: 99%

Wastewater as a fertility source for novel bacteriophages against multi-drug resistant bacteria

Alharbi

Ziadi

2021

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

Antibiotic resistance is a common and serious public health worldwide. As an alternative to antibiotics, bacteriophage (phage) therapy offers one of the best solutions to antibiotic resistance. Bacteriophages survive where their bacterial hosts are found; thus, they exist in almost all environments and their applications are quite varied in the medical, environmental, and industrial fields. Moreover, a single phage or a mixture of phages can be used in phage therapy; mixed phages tend to be more effective in reducing the number and/or activity of pathogenic bacteria than that of a single phage.

show abstract

Section: Sources Of Bacteriophagesmentioning

confidence: 99%

Wastewater as a fertility source for novel bacteriophages against multi-drug resistant bacteria

Alharbi

Ziadi

2021

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

show abstract

“…In the present study, we successfully isolated six virulent phages against MRSA from Motahari hospital sewage over the course of 6 months. In various studies, such as our study, wastewater has been used as a phage separation reservoir [27][28][29][30][31]. MH-1 titer was determined to be 1×10 9 PFU/ml therefore; hospital sewage seems to be a good source for isolation of this phage.…”

Section: Discussionmentioning

confidence: 79%

Isolation and In Vitro Evaluation of Bacteriophage against Methicillin-Resistant Staphylococcus aureus (MRSA) from Burn wounds

Hallajzadeh¹,

Mojtahedi²,

Mahabadi³

et al. 2019

Arch Clin Microbiol

View full text Add to dashboard Cite

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a major threat to human health. It is considered to be a Multi-Drug Resistant (MDR) pathogen capable of causing a variety of diseases. MRSA is also one of the most important nosocomial pathogens in burn infection. As a treatment strategy against MRSA infections phage therapy has the potential of becoming alternative remedy. Thus, the aim of present study was to isolate and characterize lytic bacteriophage from hospital sewage effective against burn wounds infecting MRSA isolates.Materials and methods: Staphylococcus aureus strains were isolated from hospitalized burnt patients. Strains were confirmed as MRSA by Kirby-Bauer disk diffusion tests to penicillin, methicillin, and oxacillin and also PCR assay for mecA gene. Phage was isolated from hospital sewage sample and tittered by Double Layer Agar (DLA) method. Spot test was used for host range determination. The latent period and burst size were estimated from one step growth curve. Phage morphology was observed by electron microscopy. The nature of the nucleic acid of isolated bacteriophage was confirmed by using Rnase A, Dnase I and 6 restriction enzymes. Results:The titer, latent period and burst size of the isolated phage was determined to be 1×10 9 PFU/ml, 20 min and 190 PFU per infected cell, respectively. It displayed a wide host range for MRSA bacteria by spot test (27 isolate from 30).Electron microscopy observation demonstrated that the phage belonged myoviridea family. Digestion profiles of Rnase A, Dnase I and XbaI restriction enzyme on 1% agarose gel Electrophoresis, show that the genome of isolated phage was a double-stranded DNA with a size of <∼43 kbp. Conclusion:The isolated phage was active against a wide range of MRSA strains recovered from burnt patients.Therefore it can be considered as a suitable candidate for use in prophylaxis and treatment of these clinical infections and for the preparation of anti-staphylococcal cocktails.

show abstract

“…In the present study, we successfully isolated six virulent phages against MRSA from Motahari hospital sewage over the course of six months. In various studies, such as our study, wastewater has been used as a phage isolation reservoir (29)(30)(31)(32)(33). The MH-1 titer was determined to be 1 × 10 9 PFU/mL; therefore, hospital sewage seems to be a good source for the isolation of this phage.…”

Section: Discussionmentioning

confidence: 84%

Characterizing a Lytic Bacteriophage Infecting Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated From Burn Patients

Hallajzadeh

Mojtahedi

Amirmozafari

et al. 2020

Arch Clin Infect Dis

View full text Add to dashboard Cite

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a significant threat to human health. It is a multidrug-resistant (MDR) pathogen capable of causing a variety of diseases. Also, MRSA is one of the most important nosocomial pathogens in burn infection. As a treatment strategy against MRSA infections, phage therapy has the potential of becoming an alternative remedy. Objectives: The present study aimed to isolate and characterize a lytic bacteriophage from hospital sewage to be effective against burn wound-infecting MRSA isolates. Methods: Staphylococcus aureus strains were isolated from hospitalized burn patients. The strains were confirmed as MRSA by the Kirby-Bauer disk diffusion method using penicillin, methicillin, and oxacillin, as well as the PCR assay for the mecA gene. The phage was isolated from the hospital sewage and tittered by the double layer agar (DLA) method. The spot test was used for host range determination. The latent period and burst size were estimated from a one-step growth curve. The phage morphology was observed by electron microscopy. The nature of the nucleic acid of the isolated bacteriophages was confirmed by Rnase A, Dnase I, and six restriction enzymes. Results: The titer, latent period, and burst size of the isolated phage were determined to be 1 × 10 9 PFU/mL, 20 min, and 190 PFU per infected cell, respectively. It displayed a broad host range for MRSA bacteria by the spot test (27 out of 30 isolates). Electron microscopy observation demonstrated that the phage belonged to the Myoviridea family. Digestion profiles of Rnase A, Dnase I, and six restriction enzymes in 1% agarose gel electrophoresis showed that the genome of the isolated phage was a double-stranded DNA with a size of <~23 kbp. Conclusions: The isolated phage (MH-1) was active against a wide range of MRSA strains recovered from burn patients. Its specificity and remarkable lytic effects on MRSA strains emphasized that it could be a suitable candidate for use in prophylaxis and treatment of these clinical infections.

show abstract

Isolation of Yersinia enterocolitica Bacteriophage From Hospital Wastewater

Cited by 6 publications

References 17 publications

Wastewater as a fertility source for novel bacteriophages against multi-drug resistant bacteria

Wastewater as a fertility source for novel bacteriophages against multi-drug resistant bacteria

Isolation and In Vitro Evaluation of Bacteriophage against Methicillin-Resistant Staphylococcus aureus (MRSA) from Burn wounds

Characterizing a Lytic Bacteriophage Infecting Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated From Burn Patients

Contact Info

Product

Resources

About