Isolation of yeasts and enteric bacteria in root‐filled teeth with chronic apical periodontitis

Pečiulienė, Vytautė; Reynaud, A.; Balciuniene, Irena; Haapasalo, Markus

doi:10.1046/j.1365-2591.2001.00411.x

Cited by 349 publications

(355 citation statements)

References 21 publications

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“…Anaerobic bacteria, especially Gram-negative species of Prevotella and Porphyromonas, are frequently associated with signs and symptoms of endodontic origin [2][3][4], and facultative species such as Enterococcus faecalis have been isolated in high frequency from refractory cases [5]. E. faecalis is rarely observed in cases of primary infections, but has been found to be the major component of microfl ora in cases of retreatment, amounting to as much as 70% of the microbiota isolated in these cases [6].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of essential oils and chloroform alone and combinated with cetrimide againstEnterococcus faecalisbiofilm

Martos¹,

Ferrer-Luque²,

González-Rodrı́guez³

et al. 2013

European Journal of Microbiology and Immunology

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The Enterococcus faecalis bacteria have been identified as the most commonly recovered species from teeth with persistent endodontic infections. The antimicrobial activity of essential oils and chloroform (CHL), alone and in association with various concentrations of cetrimide (CTR), against biofilm of Enterococcus faecalis was investigated. Solutions of CHL, eucalyptus oil (EO) and orange oil (OO) associated with CTR at 0.3%, 0.2%, 0.1%, and 0.05% were used to determine antimicrobial activity by exposing treated bovine dentine blocks to E. faecalis. Biofilms grown in the dentine blocks for 7 days were exposed to solutions for 2 and 5 min. Biofilm reduction between OO and EO at 2 min did not show any significant differences; however, OO had a higher kill percentage of biofilms than did the eucalyptus oil at 5 min (p < 0.01). Combinations with CTR at all concentrations achieved a 100% kill rate at 2 and 5 min. The association of CTR with solvent agents achieved the maximum antimicrobial activity against E. faecalis biofilms in dentine.

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Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of essential oils and chloroform alone and combinated with cetrimide againstEnterococcus faecalisbiofilm

Martos¹,

Ferrer-Luque²,

González-Rodrı́guez³

et al. 2013

European Journal of Microbiology and Immunology

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“…In cases of pulp necrosis with radiographic sign of apical periodontitis and lack of periodontal disease, E. faecalis was isolated in 55% (4) and 64% of the cases (2). Microbiological identification and analytic studies of teeth presenting endodontic treatment and periapical lesion verified that this microorganism is the most prevalent species, present in 45% (3), 77% (5) and up to 77.8% (6) of the cases.…”

Section: Introductionmentioning

confidence: 91%

“…However, Bystrom and Sundqvist (1) showed that microorganisms can survive even after biomechanical preparation and Enterococcus faecalis (Gram-positive, facultative anaerobic coccus) has been frequently isolated in root canals exhibiting persistent post-endodontic treatment infections (2,3).…”

Section: Introductionmentioning

confidence: 99%

Different Extracts of Zingiber officinale Decrease Enterococcus faecalis Infection in Galleria mellonella

et al. 2015

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Dried, fresh and glycolic extracts of Zingiber officinale were obtained to evaluate the action against G. mellonella survival assay against Enterococcus faecalis infection. Eighty larvae were divided into: 1) E. faecalis suspension (control); 2) E. faecalis + fresh extract of Z. officinale (FEO); 3) E. faecalis + dried extract of Z. officinale (DEO); 4) E. faecalis + glycolic extract of Z. officinale (GEO); 5) Phosphate buffered saline (PBS). For control group, a 5 μL inoculum of standardized suspension (10 7 cells/mL) of E. faecalis (ATCC 29212) was injected into the last left proleg of each larva. For the treatment groups, after E. faecalis inoculation, the extracts were also injected, but into the last right proleg. The larvae were stored at 37 °C and the number of dead larvae was recorded daily for 168 h (7 days) to analyze the survival curve. The larvae were considered dead when they did not show any movement after touching. E. faecalis infection led to the death of 85% of the larvae after 168 h. Notwithstanding, in treatment groups with association of extracts, there was an increase in the survival rates of 50% (GEO), 61% (FEO) and 66% (DEO) of the larvae. In all treatment groups, the larvae exhibited a survival increase with statistically significant difference in relation to control group (p=0.0029). There were no statistically significant differences among treatment groups with different extracts (p=0.3859). It may be concluded that the tested extracts showed antimicrobial activity against E. faecalis infection by increasing the survival of Galleria mellonella larvae.

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“…17,51,52 Enterococci, which are seldom found in these circumstances, have been isolated from teeth undergoing endodontic treatment and previously root treated teeth. [53][54][55][56] In a study of root filled teeth with apical periodontitis 69% of the bacterial strains recovered from the root canals were faculative anaerobes. 53,54 Enterococci were isolated in 50% of the infective cases and were the most frequently isolated.…”

Section: Endodonticsmentioning

confidence: 99%

Clindamycin and dentistry

Addy

Martin²

2005

Br Dent J

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Isolation of yeasts and enteric bacteria in root‐filled teeth with chronic apical periodontitis

Abstract: The high prevalence of enteric bacteria and yeasts in root-filled teeth with chronic apical periodontitis was established. IKI improved the antimicrobial effect of the treatment.

Cited by 349 publications

References 21 publications

Antimicrobial activity of essential oils and chloroform alone and combinated with cetrimide againstEnterococcus faecalisbiofilm

Antimicrobial activity of essential oils and chloroform alone and combinated with cetrimide againstEnterococcus faecalisbiofilm

Different Extracts of Zingiber officinale Decrease Enterococcus faecalis Infection in Galleria mellonella

Clindamycin and dentistry

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