Isolation of Virus Strains From Mosquitoes Collected in Queensland, 1972–1976

Abstract

Sttmmtry.Ml.MS mosquitoes and 4^53 ofhcr anhropods coltcctetJ at three cenires in QueenslaiKJ in I972>1976 yiekfcd ISl strains or 18 viruses. CuJex annuiirossm was the m^jor source of virus noUtion but 42 strains from AeJes nurmoitemis indicate it IO Iw a vector of importance. Ross River and Kokobera viruses *trc isdatod at Kownnyama in the dry season, a finding of inicmt a^ being compariNc %kith yearround \urvival in vector-vertebrate cyiiies. Cule.v fatigam has in pan replaced Culex an/iuliroitrii in pchdomestic breedmg sites at Kouanyama: the inrrcquency or virus botatlon from ii ^uggc^I^ that this replacement may lower arK>viriis infcLtian rales. Twelve Mrainv were iJcntified as viruses antigenically diMlnci from any previously Motnted in Australia or NCNV Guinea: 0)16129. shown by the Intcrniitional Reference Centre for Arboviruses to be a previously undescribed nKnibcr of the Sinibu Group (f-awy's Paddock virus). Chl631.1 (Murwch). ChI9.S20 (Parker's I arm) iitid Chl9546 (Little Sussex). The remaining strains were idcntilicd ns viruses previously known in Au-ilrnthi, hut included many new host or geographical record**.

Isolation of Murray Valley Encephalitis Virus and Other Arboviruses in the Ord River Valley 1972–1976

“…annuUrostris at the end of the dry were isolated in 1974. These dry season isolations add to the evidence that MVE and other viruses may be maintained in permanent transmission cycles in tropical Australia (Doherty et al, 1979). The predominance of end-of-wet season activity is a feature of all arbovirus studies in Australia (Doherty et al, 1979;Marshall, 1979).…”

“…These dry season isolations add to the evidence that MVE and other viruses may be maintained in permanent transmission cycles in tropical Australia (Doherty et al, 1979). The predominance of end-of-wet season activity is a feature of all arbovirus studies in Australia (Doherty et al, 1979;Marshall, 1979). Additional detailed collecting and analysis are needed to further define these maintenance cycles in the Ord Valley and to analyse the occurrence of isolates in the dry season.…”

“…During the same period, studies in Queensland yielded only six strains of MVE from a total of 171,348 mosquitoes (Doherty et al, 1979). The reproducibility and intensity of the Ord River activity strongly suggests that these viruses are endemically maintained in the Kimberley region.…”

“…However, despite intensive study, MVE was not recovered again at Kowanyama until 1972and then again in 1975(Doherty et al, 1979. A single isolate was also obtained from Charleville (Southwestern Queensland) during the 1974 epidemic in eastern Australia (Doherty et al, 1979).…”

Isolation of Arboviruses from Insects Collected at Beatrice Hill, Northern Territory of Australia, 1974?1976

“…Those viruses which were negative in this test were tested at The Queensland Institute of Medical Research (QIMR) by standard methods (Doherty et al 1979) against antisera to all known Australian arboviruses (Doherty 1972(Doherty , 1977. Viruses which did not react with these antisera were submitted to the WHO International Reference Centre, Yale Arbovirus Research Unit.…”