Isolation of Two Strains of a New Tombusvirus (Havel river virus, HaRV) from Surface Waters in Germany

Koenig, R.; Pfeilstetter, Ernst; Kegler, H.; Lesemann, D.‐E.

doi:10.1023/b:ejpp.0000021084.38938.c3

Cited by 10 publications

(11 citation statements)

References 13 publications

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“…However, even with good quality antisera, the sensitivity of the double-antibody-sandwich ELISA (DAS-ELISA) procedure is limited to detecting plant viruses down to 1e10 ng ml À1 (Bergmayer, 1986), while the reverse transcriptase polymerase chain reaction (RT-PCR) usually enables a further 100-to 1000-fold increase in sensitivity (Candresse et al, 1998). Using a relatively new important diagnostic method in plant virology, real-time PCR (RT-qPCR) Ló pez et al, 2006;Agindotan et al, 2007;Ling et al, 2007;Kogov sek et al, 2008;Gutierrez-Aguirre et al, 2009b;Cepin et al, 2010), concentrations as low as 10 genome copies of viruses per reaction can be reliably detected (Gutierrez-Aguirre et al, 2009b).…”

Section: Methods For Detecting Plant Viruses In Watermentioning

confidence: 99%

“…Apart from viruses known to be plant pathogens, novel infectious viruses from genus Carmovirus (Yi et al, 1992), Potexvirus (Koenig and Lesemann, 1985;Bü ttner and Nienhaus, 1989), Tombusvirus (Yi et al, 1992;Koenig and Lesemann, 1985;Gallitelli et al, 1989;Koenig et al, 2004) and Tobamovirus (Koenig and Lesemann, 1985) have been isolated from environmental waters. Their natural hosts and importance in nature are unknown.…”

Section: Van Dorst Reported Viruses In Irrigation Waters (Cited Inmentioning

confidence: 99%

“…Their natural hosts and importance in nature are unknown. They are usually named after the river or place where first isolated, e.g., Sikte waterborne virus (SWBV), Ahlum waterborne virus (AWBV) and Weddel waterborne virus (WWBV) (Yi et al, 1992), Neckar river virus (NRV; Tombusvirus Neckar; Koenig and Lesemann, 1985), Lato river virus (LRV) (Gallitelli et al, 1989), Havel river virus (HaRV) (Koenig et al, 2004).…”

Section: Van Dorst Reported Viruses In Irrigation Waters (Cited Inmentioning

confidence: 99%

“…The risk of virus spread in agriculture and horticulture can be reduced by regular monitoring of seeds and seedlings, using the very sensitive diagnostic methods that are necessary, especially for diagnosis of latent infection, such as real time PCR (Ló pez et al, 2006;Agindotan et al, 2007;Ling et al, 2007;Kogov sek et al, 2008;Gutierrez-Aguirre et al, 2009b). Appropriate hygiene measures, e.g., disinfection of tools and workers' clothing, and removal of crop residues, are required.…”

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confidence: 99%

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Plant viruses in aqueous environment – Survival, water mediated transmission and detection

Mehle

Ravnikar

2012

Water Research

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Section: Methods For Detecting Plant Viruses In Watermentioning

confidence: 99%

Section: Van Dorst Reported Viruses In Irrigation Waters (Cited Inmentioning

confidence: 99%

Section: Van Dorst Reported Viruses In Irrigation Waters (Cited Inmentioning

confidence: 99%

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confidence: 99%

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Plant viruses in aqueous environment – Survival, water mediated transmission and detection

Mehle

Ravnikar

2012

Water Research

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“…A sequence motif of E, P, and W, is conserved between aa 199-206 across the coat protein of all known tombusviruses, except CIRV (Koenig et al 2004). This distinguishing feature also was observed in both TuCV and ManRV, confirming that they were members of the tombusvirus genus.…”

Section: Discussionmentioning

confidence: 99%

New tombusviruses isolated from surface waters in New Zealand

Mukherjee

Lough²,

Hopcroft

et al. 2011

Australasian Plant Pathol.

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Water samples from Turitea Creek and the Manawatu River near Palmerston North, New Zealand were assayed for infectious plant viruses. Twenty liter water samples each were prefiltered and virions adsorbed onto electropositive Zeta Plus 50S membranes. Eluates were examined for virions by transmission electron microscopy. Icosahedral particles with a diameter of 30 nm with no distinct capsomere arrangement were observed. Two distinct tombusvirus isolates were suspected based on symptoms in Vigna unguiculata. An A 260/280 =1.64, and a buoyant density in CsCl=1.35 supported the conclusion that both isolates were tombusviruses. Maximum Parsimony trees generated from the deduced amino acid sequence of an 820 bp amplicon within the p33 (RdRp) gene and the entire capsid protein gene (1100 bp) showed maximum similarity of the isolates from Manawatu river and Turitea creek with Cucumber bulgarian latent virus (60% and 64%, respectively) in the p33 region, and with Maize necrotic streak virus (83%) and Cymbidium ringspot virus (76%), respectively, in the capsid protein region. In accordance with the species demarcation criteria of <87% sequence identity in the capsid protein amino acid sequences established by the ICTV, it is suggested that both isolates are new tombusviruses, for which we propose the names Turitea creek virus (TuCV) and Manawatu river virus (ManRV). This is the first report of tombusviruses in New Zealand.

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Evaluation of various species demarcation criteria in attempts to classify ten new tombusvirus isolates

et al. 2004

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The usefulness of various suggested species demarcation criteria was compared in attempts to determine the taxonomic status of ten new tombusvirus isolates. Five of them (Lim 1, 2, 3, 5 and 6) were obtained from different sources of commercially grown statice (Limonium sinuatum), two (Gyp 1 and 2) from different sources of commercially grown Gypsophila paniculata and three from water samples, i.e. from a small river (Schunter) in Northern Germany, from a brook (near Dossenheim) in Southern Germany and from the groundwater in a Limonium production glasshouse in the Netherlands (Lim 4). The immunoelectron microscopical decoration test allowed a quick preliminary assignment of various isolates to several known tombusviruses. A more precise analysis of the relationships was achieved by comparing the deduced amino acid sequences of the coat proteins. Sequence as well as serological data suggested that eight of the isolates should be classified as strains or variants of either Carnation Italian ringspot virus, Grapevine Algerian latent virus, Petunia asteroid mosaic virus or Sikte waterborne virus, respectively, whereas the 9th isolate (Lim 2) appears to represent a distinct new tombusvirus species. The case of the 10th isolate (Lim 5) illustrates the classification problems experienced when the properties of a virus place it close to the more or less arbitrary man-made borderline between virus species and virus strains. The coat protein gene sequences were also determined for some viruses for which these data had not yet been available, i.e. Neckar river virus, Sikte waterborne virus and Eggplant mottled crinkle virus. The sequences of the coat protein gene and also of ORF 1 of the latter virus proved to be almost identical to the corresponding genome regions of the recently described Pear latent virus, which for priority reasons should be renamed. Criteria which have been suggested in addition to serology and sequence comparisons for tombusvirus species demarcation, i.e. differences in natural and in experimental host ranges, in cytopathological features and in coat protein size, appear to be of little value for the classification of new tombusviruses.

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Isolation of Two Strains of a New Tombusvirus (Havel river virus, HaRV) from Surface Waters in Germany

Cited by 10 publications

References 13 publications

Plant viruses in aqueous environment – Survival, water mediated transmission and detection

Plant viruses in aqueous environment – Survival, water mediated transmission and detection

New tombusviruses isolated from surface waters in New Zealand

Evaluation of various species demarcation criteria in attempts to classify ten new tombusvirus isolates

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