Isolation of two neuropeptides in the AKH/RPCH-family from horseflies (diptera)

Jaffe, Howard; Raina, Ashok K.; Fraser, Blair A.; Keim, Pamela S.; Rao, K. Ranga; Zhang, Yao-Shi; Lancaster, Jill; Hayes, D. K.

doi:10.1016/s0006-291x(88)80331-0

Cited by 15 publications

(2 citation statements)

References 9 publications

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“…Even though the collection represented 19 species in four genera, a majority of the flies were T. atratus, and chromatographic evidence indicated that the two peptides were identical across the species. Isolation of the peptides has been described earlier (2). Since Tabanids cannot presently be reared in the laboratory, and the physiological status of fieldcollected flies was unknown, laboratory-reared face fly (Musca autumnalis) adults (a species from the same order of insects) were used for the bioassays.…”

Section: Methodsmentioning

confidence: 99%

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Primary structure of two neuropeptide hormones with adipokinetic and hypotrehalosemic activity isolated from the corpora cardiaca of horse flies (Diptera).

Jaffe

Raina

Riley

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

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Section: Methodsmentioning

confidence: 99%

“…11 Both peptides were purified by a sequence of three reversed-phase HPLC steps. Amino acid composition, spectral analysis, and preliminary biological data clearly placed these peptides in the AKH/RPCH family (2). This report describes the elucidation of the primary structure, chemical syntheses, and biological activity of Taa-AKH and Taa-HoTH.…”

mentioning

confidence: 87%

Primary structure of two neuropeptide hormones with adipokinetic and hypotrehalosemic activity isolated from the corpora cardiaca of horse flies (Diptera).

Jaffe

Raina

Riley

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

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Insect neuropeptides: Discovery and application in insect management

Masler

Kelly

Menn

1993

Arch Insect Biochem Physiol

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New approaches to the development of insect control agents have been revealed through the molecular description of neuropeptides, their biogenesis, action, and degradation. Prerequisite to the exploitation of a neuropeptide as a lead to control agent development is a thorough understanding of the biochemistry of the neuropeptide and appreciation of its physiological impact. Reliable bioassays must be coupled with advanced biochemical and molecular genetic technologies to overcome limitations imposed by the typically low endogenous levels of individual neuropeptides. Purification, amino acid sequencing, and gene cloning provide the molecular tools necessary for studies on neuropeptide synthesis, processing, secretion, receptor binding, and inactivation. Each of these areas consists of a number of amino acid sequence-, and enzyme-dependent steps which may be considered as targets for the development of highly specific control agents. These agents will include antagonist and superagonists, peptidomimetics, recombinant peptides delivered through the baculovirus technology, receptor blockers, and enzyme inhibitors.

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