Isolation of two lapine rotaviruses: Characterization of their subgroup, serotype and RNA electropherotypes

Abstract

Rotaviruses were detected by an ELISA test in stool specimens from diarrheic rabbits in two commercial rabbitries and cultured in MA 104 cells. Their identity was confirmed by electron microscopy and indirect immunofluorescence. They were found to belong to subgroup I by testing with monoclonal antibodies and to serotype 3 by neutralization with homologous and heterologous antisera. Although both viruses were neutralized by antiserum to human serotype 3 the ALA rabbit rotavirus was minimally neutralized by antiserum to the C11 rabbit rotavirus. Electrophoresis of viral RNA revealed 11 segments characteristic of rotavirus, however both rabbit rotaviruses had unusual electropherotypes. They differed from each other with greatly reduced mobility of the tenth segment in one virus and the eleventh segment in the other virus.

Frequent rearrangement may explain the structural heterogeneity in the 11th genome segment of lapine rotaviruses — Short communication

“…The sequence of genome segment 11 of these two lapine strains was determined and analysed along with rearranged and normal cognate genome segments of other rotavirus strains of lapine origin. Two additional lapine strains, Alabama with super-short E-type (isolated in the USA in 1984; Thouless et al, 1986) and 30/96 with long E-type (isolated in Italy in 1996; Martella et al, 2003), and the human strain, B4106 (De Leener et al, 2004), representing a zoonotic, lapine-derived P[14],G3 rotavirus (Matthijnssens et al, 2006a), were also included in this study for comparison.…”

“…Such super-short Etype has been observed rarely in bovine (VMRI), porcine (MC345, CC86, CC117, C60) simian (YK-1) and human (69M, B37, B38, AU19, Z10262) strains (Paul et al, 1988;González et al, 1989;Nuttall et al, 1989;Scott et al, 1989;Mattion et al, 1990;Ciarlet et al, 1997;Palombo et al, 1998;Nakagomi et al, 1999;Westerman et al, 2006). In addition, super-short RNA profile has been observed frequently in lapine rotaviruses (ALA, BAPwt, BAP-2, 3489/3, LRV-4; Thouless et al, 1986;Tanaka et al, 1988;Bányai et al, 2005;Martella et al, 2005) and in a lapine-like human strain, B4106, detected in Belgium (De Leener et al, 2004;Matthijnssens et al, 2006a). In addition, some tissue-culture adapted lapine rotavirus (LRV) strains has atypical long genome pattern (strain C-11) due to abnormal mobility of genome segment 10 or a short pattern (strain R-2) due to slower migration of genome segment 11 (Thouless et al, 1986).…”

“…In addition, super-short RNA profile has been observed frequently in lapine rotaviruses (ALA, BAPwt, BAP-2, 3489/3, LRV-4; Thouless et al, 1986;Tanaka et al, 1988;Bányai et al, 2005;Martella et al, 2005) and in a lapine-like human strain, B4106, detected in Belgium (De Leener et al, 2004;Matthijnssens et al, 2006a). In addition, some tissue-culture adapted lapine rotavirus (LRV) strains has atypical long genome pattern (strain C-11) due to abnormal mobility of genome segment 10 or a short pattern (strain R-2) due to slower migration of genome segment 11 (Thouless et al, 1986).…”

Genome rearrangements in porcine rotaviruses: biochemical and biological comparisons between a supershort strain and its standard counterpart

“…Rotaviruses with genome rearrangements have been reported in humans, calves, rabbits and pigs (Pedley et al, 1984;Thouless et al, 1986;Besselaar et al, 1986;Bellinzoni et al, 1987;Pocock, 1987;Tanaka et al, 1988). They have also been generated in tissue culture after serial passage at high m.o.i.…”

Structure of Rearranged Genome Segment 11 in Two Different Rotavirus Strains Generated by a Similar Mechanism

“…Rotaviruses with genome rearrangements have been reported in humans, calves, rabbits and pigs (Pedley et al, 1984;Besselaar et al, 1986;Pocock, 1987;Thouless et al, 1986;Bellinzoni et al, 1987). They have also been generated in tissue culture after serial passage at high multiplicity of infection (Hundley et al, 1985).…”