Isolation of toxoplasma gondii from immunocompromised patients using tissue culture

Calicó, I; Caballero, Estrella; Martínez, OA; Arce, L. Arcalis; Llopart, L.; Ocaña, Inmaculada; C, Juste

doi:10.1007/bf01645360

Cited by 13 publications

(3 citation statements)

References 8 publications

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“…Toxoplasma can be identified histologically or isolated in tissue cultures. Isolation of T. gondii from muscle tissue in cases of myositis has been reported (66). The immunohistochemistry using antisera to T. gondii is both a sensitive and specific diagnostic marker (67).…”

Section: Myositis Due To Parasitic Infectionsmentioning

confidence: 99%

Infective myositis

Gayathri

Nandeesh

2021

Brain Pathology

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Myositis is inflammation especially of the voluntary muscles, characterized by localized or diffuse pain, tenderness on movement or palpation, swelling, and/or weakness. The two main categories of myositis include non‐infectious and infectious. Infective myositis may be due to a wide variety of pathogens, including bacteria, fungi, viruses, and parasites. A brief account of the various pathogens causing infective myositis is discussed.

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Section: Myositis Due To Parasitic Infectionsmentioning

confidence: 99%

Infective myositis

Gayathri

Nandeesh

2021

Brain Pathology

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“…Isolation of Toxoplasma in tissue culture or identification histologically can be performed. For instance, isolation of T. gondii from the muscle tissue in cases of myositis has been described (32). Treatment is generally not warranted, as infection is usually self-limited.…”

Section: Toxoplasma Gondiimentioning

confidence: 99%

Bacterial, Fungal, Parasitic, and Viral Myositis

2008

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SUMMARY Infectious myositis may be caused by a broad range of bacterial, fungal, parasitic, and viral agents. Infectious myositis is overall uncommon given the relative resistance of the musculature to infection. For example, inciting events, including trauma, surgery, or the presence of foreign bodies or devitalized tissue, are often present in cases of bacterial myositis. Bacterial causes are categorized by clinical presentation, anatomic location, and causative organisms into the categories of pyomyositis, psoas abscess, Staphylococcus aureus myositis, group A streptococcal necrotizing myositis, group B streptococcal myositis, clostridial gas gangrene, and nonclostridial myositis. Fungal myositis is rare and usually occurs among immunocompromised hosts. Parasitic myositis is most commonly a result of trichinosis or cystericercosis, but other protozoa or helminths may be involved. A parasitic cause of myositis is suggested by the travel history and presence of eosinophilia. Viruses may cause diffuse muscle involvement with clinical manifestations, such as benign acute myositis (most commonly due to influenza virus), pleurodynia (coxsackievirus B), acute rhabdomyolysis, or an immune-mediated polymyositis. The diagnosis of myositis is suggested by the clinical picture and radiologic imaging, and the etiologic agent is confirmed by microbiologic or serologic testing. Therapy is based on the clinical presentation and the underlying pathogen.

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“…The doublesandwich IgM ELISA and IgM immunosorbent agglutination assay can be used for detection of IgM antibodies that confirms the acute infection (Sensini 2006). Isolation of T. gondii from blood, muscle tissue in case of myositis (Calicó et al 1991), shows that infection is acute (Crum-Cianflone 2008). The immunoperoxidase technique, which uses antisera to T. gondii, has proven to be both sensitive and specific and is superior to conventionally stained tissue sections (Abedalthagafi et al 2010).…”

Section: Protozoa Causing Myositismentioning

confidence: 98%

Parasitic infections and myositis

et al. 2011

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Infectious myositis may be caused by a wide variety of bacterial, fungal, viral, and parasitic agents. Parasitic myositis is most commonly a result of trichinosis, cystericercosis, or toxoplasmosis, but other parasites may be involved. A parasitic cause of myositis is suggested by history of residence or travel to endemic area and presence of eosinophilia. The diagnosis of parasitic myositis is suggested by the clinical picture and radiologic imaging, and the etiologic agent is confirmed by parasitologic, serologic, and molecular methods, together with histopathologic examination of tissue biopsies. Therapy is based on the clinical presentation and the underlying pathogen. Drug resistance should be put into consideration in different geographic areas, and it can be avoided through the proper use of anti-parasitic drugs.

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Isolation of toxoplasma gondii from immunocompromised patients using tissue culture

Cited by 13 publications

References 8 publications

Infective myositis

Infective myositis

Bacterial, Fungal, Parasitic, and Viral Myositis

Parasitic infections and myositis

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