Isolation of Tobacco Mosaic Virus‐Binding Peptides for Biotechnology Applications

Chan, Soo Khim; Steinmetz, Nicole F.

doi:10.1002/cbic.202200040

Cited by 2 publications

(4 citation statements)

References 25 publications

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“…TMV, CCMV, and PVX were propagated in plants and purified using our established protocols. Bacteriophage Qβ VLPs were expressed in BL21 Escherichia coli and purified as previously reported .…”

Section: Methodsmentioning

confidence: 99%

“…Bacteriophage Qβ VLPs were expressed in BL21 Escherichia coli and purified as previously reported . Characterization of the plant viruses and VLPs was reported elsewhere. ,,− …”

Section: Methodsmentioning

confidence: 99%

“…Tobacco Mosaic Virus, Potato Virus X, Chlorotic Cowpea Mosaic Virus Propagation, and Qβ VLP Production and Characterization. TMV, 89 CCMV, 90 and PVX 91 were propagated in plants and purified using our established protocols. Bacteriophage Qβ VLPs were expressed in BL21 Escherichia coli and purified as previously reported.…”

Section: Cowpea Mosaic Virus Propagation and Preparation Of Cy5-cpmvmentioning

confidence: 99%

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In Vivo Fate of Cowpea Mosaic Virus In Situ Vaccine: Biodistribution and Clearance

et al. 2022

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Cowpea mosaic virus (CPMV) is a nucleoprotein nanoparticle that functions as a highly potent immunomodulator when administered intratumorally and is used as an in situ vaccine. CPMV in situ vaccination remodels the tumor microenvironment and primes a highly potent, systemic, and durable antitumor immune response against the treated and untreated, distant metastatic sites (abscopal effect). Potent efficacy was demonstrated in multiple tumor mouse models and, most importantly, in canine cancer patients with spontaneous tumors. Data indicate that presence of anti-CPMV antibodies are not neutralizing and that in fact opsonization leads to enhanced efficacy. Plant viruses are part of the food chain, but to date, there is no information on human exposure to CPMV. Therefore, patient sera were tested for the presence of immunoglobulins against CPMV, and indeed, >50% of deidentified patient samples tested positive for CPMV antibodies. To get a broader sense of plant virus exposure and immunogenicity in humans, we also tested sera for antibodies against tobacco mosaic virus (>90% patients tested positive), potato virus X (<20% patients tested positive), and cowpea chlorotic mottle virus (no antibodies were detected). Further, patient sera were analyzed for the presence of antibodies against the coliphage Qβ, a platform technology currently undergoing clinical trials for in situ vaccination; we found that 60% of patients present with anti-Qβ antibodies. Thus, data indicate human exposure to CPMV and other plant viruses and phages. Next, we thought to address agronomical safety; i.e., we examined the fate of CPMV after intratumoral treatment and oral gavage (to mimic consumption by food). Because live CPMV is used, an important question is whether there is any evidence of shedding of infectious particles from mice or patients. CPMV is noninfectious toward mammals; however, it is infectious toward plants including black-eyed peas and other legumes. Biodistribution data in tumor-bearing and healthy mice indicate little leaching from tumors and clearance via the reticuloendothelial system followed by biliary excretion. While there was evidence of shedding of RNA in stool, there was no evidence of infectious particles when plants were challenged with stool extracts, thus indicating agronomical safety. Together these data aid the translational development of CPMV as a drug candidate for cancer immunotherapy.

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Section: Methodsmentioning

confidence: 99%

“…Bacteriophage Qβ VLPs were expressed in BL21 Escherichia coli and purified as previously reported . Characterization of the plant viruses and VLPs was reported elsewhere. ,,− …”

Section: Methodsmentioning

confidence: 99%

Section: Cowpea Mosaic Virus Propagation and Preparation Of Cy5-cpmvmentioning

confidence: 99%

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In Vivo Fate of Cowpea Mosaic Virus In Situ Vaccine: Biodistribution and Clearance

et al. 2022

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“…Both proteins are directly translated from the genomic RNA, which ends with a terminal tRNA-like structure that specifically aminoacylates with histidine. The 5′-NTR serves as a strong translational enhancer [ 30 ]. Both the 5′- and 3′-NTRs contain cis-acting elements essential for replication [ 31 ].…”

Section: Tobacco Mosaic Virus (Tmv)mentioning

confidence: 99%

Plant Virus Nanoparticles Combat Cancer

et al. 2023

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Plant virus nanoparticles (PVNPs) have garnered considerable interest as a promising nanotechnology approach to combat cancer. Owing to their biocompatibility, stability, and adjustable surface functionality, PVNPs hold tremendous potential for both therapeutic and imaging applications. The versatility of PVNPs is evident from their ability to be tailored to transport a range of therapeutic agents, including chemotherapy drugs, siRNA, and immunomodulators, thereby facilitating targeted delivery to the tumor microenvironment (TME). Furthermore, PVNPs may be customized with targeting ligands to selectively bind to cancer cell receptors, reducing off-target effects. Additionally, PVNPs possess immunogenic properties and can be engineered to exhibit tumor-associated antigens, thereby stimulating anti-tumor immune responses. In conclusion, the potential of PVNPs as a versatile platform for fighting cancer is immense, and further research is required to fully explore their potential and translate them into clinical applications.

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Isolation of Tobacco Mosaic Virus‐Binding Peptides for Biotechnology Applications

Cited by 2 publications

References 25 publications

In Vivo Fate of Cowpea Mosaic Virus In Situ Vaccine: Biodistribution and Clearance

In Vivo Fate of Cowpea Mosaic Virus In Situ Vaccine: Biodistribution and Clearance

Plant Virus Nanoparticles Combat Cancer

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