Isolation of tobacco DNA segments with plant promoter activity.

Herman, Lieve; Montagu, Marc C. Van; Depicker, Anna

doi:10.1128/mcb.6.12.4486

Cited by 19 publications

(9 citation statements)

References 31 publications

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“…This change makes this open reading frame identical to qacEAl. It should be noted that the open reading frame reported to be from A. tumefaciens (20) is not actually from this genus, but is located on an artificial cloning vector whose DNA derived originally from E. coli (23).…”

Section: Resultsmentioning

confidence: 99%

The 3' conserved segment of integrons contains a gene associated with multidrug resistance to antiseptics and disinfectants

Paulsen

Littlejohn

Rådström

et al. 1993

Antimicrob Agents Chemother

288

259

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Nucleotide sequence analysis of ORF1 from the integron on the broad-host-range plasmid R751 revealed that the first 94 of 110 codons of ORF1 from R751 are identical to ORF4, an open reading frame from the 3' conserved segment of other integrons found in gram-negative bacteria, after which point they diverged completely. The predicted products of both ORFI and ORF4 share homology with the multidrug exporter QacC. Phenotypic analysis revealed that ORFI specifies a resistance profile to antiseptics and disinfectants almost identical to that of qacC, whereas ORF4 specifies much lower levels of resistance to these compounds. ORF4, whose product lacks the C-terminal 16 amino acids of the ORF1 protein, may have evolved by the interruption of ORF1 from the insertion of a DNA segment carrying a sufl sulfonamide resistance determinant. Hence, ORF1 was designated qacE, and its partially functional deletion derivative, ORF4, was designated qacEAI. Fluorimetric experiments indicated that the mechanism of resistance mediated by QacE, the protein specified by qacE, is active export energized by proton motive force. Amino acid sequence comparisons revealed that QacE is related to a family of small multidrug export proteins with four transmembrane segments.

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Section: Resultsmentioning

confidence: 99%

The 3' conserved segment of integrons contains a gene associated with multidrug resistance to antiseptics and disinfectants

Paulsen

Littlejohn

Rådström

et al. 1993

Antimicrob Agents Chemother

288

259

View full text Add to dashboard Cite

show abstract

“…The different promoter deletions were inserted as Barn HI/Bgl II fragments into the Bgl II site upstream of the promoterless NPTII gene of the intermediate Ti vector plasmid pGV180, a derivative of pGV150 [5,22].…”

Section: Methodsmentioning

confidence: 99%

A complex ensemble of cis-regulatory elements controls the expression of a Vicia faba non-storage seed protein gene

et al. 1993

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We have identified cis-regulatory elements within the 5'-upstream region of a Vicia faba non-storage seed protein gene, called usp, by studying the expression of usp-promoter deletion fragments fused to reporter genes in transgenic tobacco seeds. 0.4 kb of usp upstream sequence contain at least six, but probably more, distinct cis-regulatory elements which are responsible for seemingly all quantitative, spatial and temporal aspects of expression. Expression-increasing and -decreasing elements are interspersed and include an AT-rich sequence, a G-box element and a CATGCATG motif. The latter acts as a negative element in contrast to what has been found for the same motif in legumin- and vicilin-type seed storage protein gene promoters. Seed specificity of expression is mainly determined by the -68/+51 region which confers, however, only very low levels of expression. The data support the combinatorial model of promoter function.

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“…These replicated T-DNAs might then preferentially integrate as an array into the plant genome. Until now, the main arguments supporting replication are that each of the T-DNAs involved in a direct or inverted repeat shows analogous breakpoints in a restriction analysis (Van Lijsebettens et al, 1986;Jorgensen et al, 1987) and that three identical T-DNA copies are present after transformation with a library of T-DNAs containing different promoters (Herman et al, 1986). In any case, the transferred singlestranded T-DNAs are made double stranded before integration (Chilton and Que, 2003) and self-replication of the newly synthesized strand might be an attractive explanation for the frequently observed inverted T-DNA repeats over the RB (Jorgensen et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

The T‐DNA integration pattern in Arabidopsis transformants is highly determined by the transformed target cell

et al. 2009

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Transgenic loci obtained after Agrobacterium tumefaciens-mediated transformation can be simple, but fairly often they contain multiple T-DNA copies integrated into the plant genome. To understand the origin of complex T-DNA loci, floral-dip and root transformation experiments were carried out in Arabidopsis thaliana with mixtures of A. tumefaciens strains, each harboring one or two different T-DNA vectors. Upon floral-dip transformation, 6-30% of the transformants were co-transformed by multiple T-DNAs originating from different bacteria and 20-36% by different T-DNAs from one strain. However, these co-transformation frequencies were too low to explain the presence of on average 4-6 T-DNA copies in these transformants, suggesting that, upon floral-dip transformation, T-DNA replication frequently occurs before or during integration after the transfer of single T-DNA copies. Upon root transformation, the co-transformation frequencies of T-DNAs originating from different bacteria were similar or slightly higher (between 10 and 60%) than those obtained after floral-dip transformation, whereas the co-transformation frequencies of different T-DNAs from one strain were comparable (24-31%). Root transformants generally harbor only one to three T-DNA copies, and thus co-transformation of different T-DNAs can explain the T-DNA copy number in many transformants, but T-DNA replication is postulated to occur in most multicopy root transformants. In conclusion, the comparable co-transformation frequencies and differences in complexity of the T-DNA loci after floral-dip and root transformations indicate that the T-DNA copy number is highly determined by the transformation-competent target cells

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Isolation of tobacco DNA segments with plant promoter activity.

Cited by 19 publications

References 31 publications

The 3' conserved segment of integrons contains a gene associated with multidrug resistance to antiseptics and disinfectants

The 3' conserved segment of integrons contains a gene associated with multidrug resistance to antiseptics and disinfectants

A complex ensemble of cis-regulatory elements controls the expression of a Vicia faba non-storage seed protein gene

The T‐DNA integration pattern in Arabidopsis transformants is highly determined by the transformed target cell

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