Isolation of three sesquiterpene lactones from the roots of Cichorium glandulosum Boiss. et Huet. by high-speed counter-current chromatography

Comparative evaluation of traditional prescriptions from Cichorium intybus L. for wound healing: Stepwise isolation of an active component by in vivo bioassay and its mode of activity

“…Besides these compounds, several polysaccharides including homogalacturonan, rhamnogalacturonan-I, cellulose, xyloglucan, heteroxylan and glucomannan have been determined in the leaves of C. intybus (Sun et al, 2006). Whereas, the roots were found to be rich in sesquiterpene lactones, coumarins and inulins (Hazra et al, 2002;Kisiel and Michalska, 2006;Wu et al, 2007).…”

ISOLATION OF FIVE BIOACTIVE COMPONENTS FROM EUPATORIUM ADENOPHORUM SPRENG USING STEPWISE ELUTION BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY

“…In order to achieve an efficient resolution of target compounds, a two-phase solvent system composed of ethyl acetate–methanol–water was examined using semi-preparative HSCCC by varying the mutual volume ratio, since this solvent system has been successfully applied to separate various samples with a moderate degree of polarity [13–16]. In the present study, the K values of the five compounds were determined for selecting suitable solvent systems (Table 1).…”

Centrifugal partition chromatography elution gradient for isolation of sesquiterpene lactones and flavonoids from Anvillea radiata

“…The corresponding partition coefficient values indicated that under these conditions, both compounds passed mainly in the organic phase (K D value from 0.1 to 0.7), meaning that these systems are not suitable to achieve a satisfactory separation. Previous studies on the CPC separation of various sesquiterpene lactones [31,32] recommended Arizona systems consequently; four of them corresponding to the polarity of A. Radiata compounds were evaluated: the F, G, H and K Arizona systems (systems 3-6 in Table 1). The best partitions were observed for systems F (1:5:1:5) and G (1:4:1:4) with K D in the range 1-6 allowing a good retention of compounds and reasonable fractionation time.…”