Isolation of the rat gene for Mullerian inhibiting substance

Haqq, Christopher M.; Lee, Mary; Tizard, Richard; Wysk, Mark Allen; DeMarinis, Janice; Donahoe, Patricia K.; Cate, Richard L.

doi:10.1016/0888-7543(92)90291-y

Cited by 57 publications

(31 citation statements)

References 22 publications

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“…The gene of sb-AMH contains unique features that distinguishes it from mammalian (Cate et al, 1986;Münsterberg and LovellBadge, 1991;Haqq et al, 1992) and bird AMH (Carré- Eusèbe et al, 1996), suggesting a high rate of diversification during (part of) AMH evolution. Mammalian and bird AMH consist of five exons, whereas sb-AMH (this study) and zebrafish AMH (Rodríguez-Marí et al, 2005) have seven.…”

Section: Discussionmentioning

confidence: 97%

Anti-Müllerian hormone (AMH/AMH) in the European sea bass: Its gene structure, regulatory elements, and the expression of alternatively-spliced isoforms

Halm

Rocha

Miura

et al. 2007

Gene

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In mammals, a multitude of studies have shown that anti-Müllerian hormone (AMH/AMH), apart from inducing Müllerian duct regression during male sexual differentiation, exerts inhibitory effects on male and female gonadal steroidogenesis and differentiation. However, in lower vertebrates like teleost fish, the function of AMH/AMH has been far less explored. As a first step to unravel its potential role in reproduction in teleost fish, we isolated and characterised the AMH gene in the European sea bass (sb), Dicentrachus labrax, determined putative regulatory elements of its 5'-flanking region, and analysed its gene expression and those of alternatively-spliced transcripts. The characterisation of sb-AMH revealed distinct features that distinguishes it from mammalian and bird AMH, suggesting a high rate of diversification of AMH during vertebrate evolution. It contained 7 exons that were divided by 6 introns, of which the last intron (intron vi) was localised only a few nucleotides upstream of the putative peptide cleavage site. The guanine and cytosine content of the open reading frame (ORF) was 52.7% and thus notably lower than that of bird and mammalian AMH. Sb-AMH cDNA was 2045 base pairs (bp) long, containing an ORF of 1599 bp encoding 533 amino acids. Deduced amino acid similarities of the conserved, carboxyterminal domain were highest with AMH in Japanese flounder (84.2%) and lowest with chicken AMH (45.5%). In the proximal promoter sequence of sb-AMH, a steroidogenic factor-1 (SF-1) binding site was present; however other regulatory sequences essential for transcriptional activation of AMH in mammals were absent. Likewise, there was no sequence homology to an SF3A2 sequence within the first 3200 bp upstream of the sb-AMH translation start site. Gene expression of sb-AMH and of alternatively-spliced sb-AMH transcripts were analysed in male and female juvenile and adult gonads as well as in somatic tissues of juvenile males. sb-AMH expression was highest in juvenile testis, but still remarkably high in juvenile ovaries and adult testis, as well as in brain, pituitary, and heart of juvenile male sea bass. Apart from adult ovary, levels of alternatively-spliced sb-AMHexonII/-99 were marginal in comparison with sb-AMH. In contrast, the transcript variant sb-AMHexonVII/+5 was expressed to a similar extent as sb-AMH in all tissues examined. The results of this work have provided the basis for future studies concerning the regulation and function of AMH/AMH in this species.

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Section: Discussionmentioning

confidence: 97%

Anti-Müllerian hormone (AMH/AMH) in the European sea bass: Its gene structure, regulatory elements, and the expression of alternatively-spliced isoforms

Halm

Rocha

Miura

et al. 2007

Gene

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“…Some of these motifs were also found conserved for both rat (Haqq et al, 1992) and bovine (Cate et al, 1986) in the 200 bp immediately upstream of the ATG available in data libraries. They were shown to interact with specific transcription factors and to be involved in AMH gene regulation (see introduction and Fig.…”

Section: The 5ј-flanking Region Of the Mouse Amh Genementioning

confidence: 90%

“…6) were then used to verify the binding specificity and relative affinity of GST-hGATA-1 for each of them in comparison with a strong affinity Ϫ180PBGD probe containing the canonical GATA element from the porphobilinogen deaminase (PBGD) gene promoter (Mignotte et al, 1989) (Fig. 6f), and by competition with specific Ϫ180PBGD and non specific (Dresser et al, 1995), rat (Haqq et al, 1992), bovine (Cate et al, 1986), and human (Guerrier et al, 1990) AMH genes. Conserved elements involved in AMH gene regulation are framed: SOX-BS (de Santa Barbara et al, 1998) and MIS-RE-1 Giuili et al, 1997).…”

Section: Binding Of Gata-1 Transcription Factor To Gatamentioning

confidence: 99%

GATA-1 is a potential repressor of anti-M�llerian hormone expression during the establishment of puberty in the mouse

et al. 2000

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“…The degeneration of the mLillerian duct in the male is induced by the antimtillerian hormone, which is a 145 kDa dimeric protein (Picard and Josso, 1984;Picard et al, 1986;Haqq et al, 1992). In the male rat, immunoreactive antimtillerian hormone protein and mRNA are first detected in the immature Sertoli cells when sex cords begin to differentiate at day 13-14 of gestation (Tran eta/., 1987;Hirobe et al, 1992).…”

Section: Sexual Differentiation Sexual Differentiation Of Reproductivmentioning

confidence: 97%

Physiology of Reproduction

2000

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Isolation of the rat gene for Mullerian inhibiting substance

Cited by 57 publications

References 22 publications

Anti-Müllerian hormone (AMH/AMH) in the European sea bass: Its gene structure, regulatory elements, and the expression of alternatively-spliced isoforms

Anti-Müllerian hormone (AMH/AMH) in the European sea bass: Its gene structure, regulatory elements, and the expression of alternatively-spliced isoforms

GATA-1 is a potential repressor of anti-M�llerian hormone expression during the establishment of puberty in the mouse

Physiology of Reproduction

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