Isolation of the fructose-1,6-bisphosphatase gene of the yeast Schizosaccharomyces pombe. Evidence for transcriptional regulation.

Vassarotti, A.; Friesen, James D.

doi:10.1016/s0021-9258(18)88978-5

Cited by 48 publications

(5 citation statements)

References 44 publications

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“…1 and 3). Furthermore, fbp1 (encoding fructose-1,6-bisphosphatase) expression is repressed in the presence of glucose (Vassarotti and Friesen 1985). Our expression analysis showed that indeed fbp1 is present in dormant spores but is silenced after refeeding (Fig.…”

Section: Temporal Kinetics Of Rna Levels During Germinationmentioning

confidence: 64%

Wake-up alarm: virtual time-lapse gene expression landscape illuminates mechanisms underlying dormancy breaking of germinating spores

2021

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Dormancy breaking is a common physiological phenomenon that is shared by eukaryotes. Germination of spores in fungi is one of the most representative cases of dormancy breaking. Understanding the mechanisms of spore germination is therefore fundamental to basic studies on the control of cell proliferation and differentiation, as well as agricultural applications and medical investigation of fungal pathogenesis. In fission yeast, spores are generated as a consequence of sexual differentiation under nutrient starvation, remaining dormant until further nourishment, but little is known about how dormant spores germinate in response to environmental change. In a breakthrough, methods for single-cell-based gene expression profiling have recently been introduced. Several mRNA expression profiles were assembled from single spore cells during dormancy or germination. Single-cell RNA-seq profiles were aligned sequentially according to their similarities. The alignment of transcriptomes visualised how gene expression varies over time upon dormancy breaking. In this review, we revisit knowledge from previous studies on germination, select candidate genes that may be involved in germination, and query their expression from the temporal transcriptomic dataset so that studies on S. pombe germination can be extended further.

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Section: Temporal Kinetics Of Rna Levels During Germinationmentioning

confidence: 64%

Wake-up alarm: virtual time-lapse gene expression landscape illuminates mechanisms underlying dormancy breaking of germinating spores

2021

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“…The fission yeast fbp1 gene has been extensively analyzed for understanding gene regulation systems (reviewed in [ 104 ]), and several promoter-associated lncRNAs are identified in its promoter region [ 59 , 105 ]. These lncRNAs are referred to as metabolic stress-induced long-ncRNA (mlonRNA) and are expressed from specific cis -element, mlon-BOX, before induction of fbp1 mRNA upon glucose starvation stress [ 106 , 107 ].…”

Section: Induction Of Meiotic Dsb Via the Expression Of Long Ncrnamentioning

confidence: 99%

Regulation Mechanisms of Meiotic Recombination Revealed from the Analysis of a Fission Yeast Recombination Hotspot ade6-M26

Hirota

2022

Biomolecules

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Meiotic recombination is a pivotal event that ensures faithful chromosome segregation and creates genetic diversity in gametes. Meiotic recombination is initiated by programmed double-strand breaks (DSBs), which are catalyzed by the conserved Spo11 protein. Spo11 is an enzyme with structural similarity to topoisomerase II and induces DSBs through the nucleophilic attack of the phosphodiester bond by the hydroxy group of its tyrosine (Tyr) catalytic residue. DSBs caused by Spo11 are repaired by homologous recombination using homologous chromosomes as donors, resulting in crossovers/chiasmata, which ensure physical contact between homologous chromosomes. Thus, the site of meiotic recombination is determined by the site of the induced DSB on the chromosome. Meiotic recombination is not uniformly induced, and sites showing high recombination rates are referred to as recombination hotspots. In fission yeast, ade6-M26, a nonsense point mutation of ade6 is a well-characterized meiotic recombination hotspot caused by the heptanucleotide sequence 5′-ATGACGT-3′ at the M26 mutation point. In this review, we summarize the meiotic recombination mechanisms revealed by the analysis of the fission ade6-M26 gene as a model system.

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“…In the fission yeast Schizosaccharomyces pombe , the fbp1 gene, which encodes fructose-1,6-bisphosphatase (FBPase), an essential enzyme for gluconeogenesis, displays dramatic transcriptional regulation and is regulated several hundred-fold by glucose concentration [ 19 ]. This enzyme is conserved from yeasts to humans.…”

Section: Signal Pathways and Transcription Factors Required For The R...mentioning

confidence: 99%

“…Mammalian FBPase activity is regulated by two metabolic inhibitors, AMP and fructose-2,6-bisphosphate [ 20 ], whereas FBPase activity in the budding yeast Saccharomyces cerevisiae is controlled by both glucose-dependent repression of gene transcription and glucose inactivation of the enzyme itself [ 21 , 22 ]. On the other hand, the FBPase activity in S. pombe is regulated only by transcriptional control but not by direct glucose inactivation of the enzyme [ 19 ], which is reminiscent of the requirement to establish systems for a quick but highly specific transcriptional response to the environmental glucose concentration.…”

Section: Signal Pathways and Transcription Factors Required For The R...mentioning

confidence: 99%

Multi-Layered Regulations on the Chromatin Architectures: Establishing the Tight and Specific Responses of Fission Yeast fbp1 Gene Transcription

Asada

Hirota

2022

Biomolecules

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Transcriptional regulation is pivotal for all living organisms and is required for adequate response to environmental fluctuations and intercellular signaling molecules. For precise regulation of transcription, cells have evolved regulatory systems on the genome architecture, including the chromosome higher-order structure (e.g., chromatin loops), location of transcription factor (TF)-binding sequences, non-coding RNA (ncRNA) transcription, chromatin configuration (e.g., nucleosome positioning and histone modifications), and the topological state of the DNA double helix. To understand how these genome-chromatin architectures and their regulators establish tight and specific responses at the transcription stage, the fission yeast fbp1 gene has been analyzed as a model system for decades. The fission yeast fbp1 gene is tightly repressed in the presence of glucose, and this gene is induced by over three orders of magnitude upon glucose starvation with a cascade of multi-layered regulations on various levels of genome and chromatin architecture. In this review article, we summarize the multi-layered transcriptional regulatory systems revealed by the analysis of the fission yeast fbp1 gene as a model system.

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Isolation of the fructose-1,6-bisphosphatase gene of the yeast Schizosaccharomyces pombe. Evidence for transcriptional regulation.

Cited by 48 publications

References 44 publications

Wake-up alarm: virtual time-lapse gene expression landscape illuminates mechanisms underlying dormancy breaking of germinating spores

Wake-up alarm: virtual time-lapse gene expression landscape illuminates mechanisms underlying dormancy breaking of germinating spores

Regulation Mechanisms of Meiotic Recombination Revealed from the Analysis of a Fission Yeast Recombination Hotspot ade6-M26

Multi-Layered Regulations on the Chromatin Architectures: Establishing the Tight and Specific Responses of Fission Yeast fbp1 Gene Transcription

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