Isolation of the chicken middle-molecular weight neurofilament (NF-M) gene and characterization of its promoter

Zopf, Dieter; Dineva, Bistra; Betz, Heinrich; Gundelfinger, Eckart D.

doi:10.1093/nar/18.3.521

Cited by 48 publications

(29 citation statements)

References 54 publications

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“…Significant developmental increases also occur in the modulatory tonus of cholinergic and catecholaminergic inputs and in the expression of brain-derived growth factor (BDNF) in the song system Saito, 1989, 1991;Akutagawa and Konishi, 1998). The expression of both chicken and rat NF-M is upregulated in neuronal cells upon treatment with neurotrophic factors (Verge et al, 1990;Zopf et al, 1990). Moreover, the expression of BDNF in the brain of juvenile zebra finches shows regional and temporal changes similar to those we describe here for NF-M (Akutagawa and Konishi, 1998), raising the possibility that BDNF might be involved in the regulation of NF-M expression.…”

Section: Developmental Regulation Of Nf-m Expressionsupporting

confidence: 76%

Enriched expression and developmental regulation of the middle‐weight neurofilament (NF‐m) gene in song control nuclei of the zebra finch

Velho

Lovell

Mello

2006

J of Comparative Neurology

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Songbirds evolved a complex set of dimorphic telencephalic nuclei that are essential for the learning and production of song. These nuclei, which together make up the oscine song control system, present several neurochemical properties that distinguish them from the rest of the telencephalon. Here we show that the expression of the gene encoding the middle-weight neurofilament (NF-M), an important component of the neuronal cytoskeleton and a useful tool for studying the cytarchitectonic organization of mammalian cortical areas, is highly enriched in large neurons within pallial song control nuclei (nucleus HVC, robustus nucleus of the arcopallium, and lateral magnocellular nucleus of the nidopallium) of male zebra finches (Taeniopygia guttata). We also show that this transcript is highly expressed in large neurons in the medulla, pons, midbrain, and thalamus. Moreover, we demonstrate that NF-M expression in song control nuclei changes during postembryonic development, peaking during an early phase of the song-learning period that coincides with the maturation of the song system. We did not observe changes in NF-M expression in auditory areas or in song control nuclei in the contexts of hearing song or singing, although these contexts result in marked induction of the transcription factor ZENK. This observation suggests that NF-M might not be under the regulatory control of ZENK in auditory areas or in song control nuclei. Overall, our data indicate that NF-M is a neurochemical marker for pallial song control nuclei and provide suggestive evidence of an involvement of NF-M in the development and/or maturation of the oscine song control system.

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Section: Developmental Regulation Of Nf-m Expressionsupporting

confidence: 76%

Enriched expression and developmental regulation of the middle‐weight neurofilament (NF‐m) gene in song control nuclei of the zebra finch

Velho

Lovell

Mello

2006

J of Comparative Neurology

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“…This residue probably was not phosphorylated in pep- (Zopf et al, 1990). All phosphopeptide fragments identified to date are indicated below the sequence, including those in the acidic region reported previously (L3-L5, V5-V7; Hollander et al, 1996) and those analyzed in the present report (refer to Fig.…”

Section: Discussionmentioning

confidence: 66%

“…We have focused on the ana!ysis of chicken NF-M. The deduced sequence of the chicken NF-M polypeptide (Zopf et al, 1990) revea!s the presence of the same motifs found to be phosphory!ated in the rat NF-M: a single KSD and four repeats of a KSP motif. The chicken NF-M protein differs from the mammalian counterparts in that it contains 17 repeats of the KXXS/TP motif, which appears on!y once in human, rat, and mouse NF-M.…”

Section: Proline-dependent Nf-m Phosphorylation Sites 535mentioning

confidence: 99%

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Identification of Ser‐Pro and Thr‐Pro Phosphorylation Sites in Chicken Neurofilament‐M Tail Domain

Bennett

Quintana²

1997

Journal of Neurochemistry

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Abstract:The tail domain of the midsize chicken neurofilament polypeptide (NF-M) contains several different types of Ser-Pro and Thr-Pro putative phosphorylation sites. We determined which of these sites are actually phosphorylated in vivo. Chick sensory neuron cultures were incubated in [ 32P]phosphate, and the cytoskeletal fraction was mixed with a neurofilament fraction prepared from adult chicken brain. NF-M was purified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and digested with chymotrypsin, and two large fragments were isolated. These were individually cleaved with trypsin, endoprotease Lys-C, or endoprotease GIu-C, and peptides separated by two-dimensional high-voltage electrophoresis and thin-layer chromatography. 32P-labeled phosphopeptides were eluted from the cellulose plates and subjected to microsequencing and mass spectometry. We found that of 21 potential Ser-Pro and Thr-Pro phosphoacceptor sites, at least 20 are phosphorylated in vivo: all four Lys-Ser-Pro sites and at least 16 of the 17 Lys-Xaa-Xaa-Ser/Thr-Pro repeats. In addition, a novel Ser-Pro site in the extreme carboxy terminus is phosphorylated. This site, which has no proximal Lys residue, is also found in mammalian NF-M, but has not been reported to be phosphorylated. Together with three casein kinase I sites we have found recently in the acidic amino-terminal segment of the tail, a total of 24 or 25 Ser and Thr phosphoacceptor sites have now been located in the chicken NF-M tail.

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“…Monteiro and Cleveland (1989) following the transfection of mouse fibroblasts with the human NF-L gene, and by Zopf et al (1990), who have described the expression of the chicken NF-M gene in transfected fibroblasts. Monteiro et al (1990) have also generated transgenic mice containing the mouse NF-L gene (pNF-L[Bam]).…”

Section: Discussionmentioning

confidence: 99%

Interaction of multiple nuclear proteins with the promoter region of the mouse 68‐kDa neurofilament gene

Ivanov¹,

Brown²

1992

J of Neuroscience Research

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Four brain-specific, DNase I hypersensitive sites (HSS) have been mapped to the 5' flanking region of the mouse 68-kDa neurofilament gene. These sites are contained within a 1.7-kb sequence that confers neuronal specificity of expression of this gene in transgenic mice. To identify DNA sequences that might be involved in gene regulation, the HSS situated near the promoter region has been analyzed by gel mobility shift assays and DNase I footprinting to investigate protein binding sequences. Of particular interest are two footprints localized to a 9-nucleotide sequence that flanks both the light and medium neurofilament gene in mouse and to a sequence that demonstrates partial homology to several promoter regions, including element-1, a motif required for neuron specificity in Drosophila. A prominent footprint was also detected at a sequence that contains a near-perfect palindrome centered at a PstI restriction site.

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Isolation of the chicken middle-molecular weight neurofilament (NF-M) gene and characterization of its promoter

Cited by 48 publications

References 54 publications

Enriched expression and developmental regulation of the middle‐weight neurofilament (NF‐m) gene in song control nuclei of the zebra finch

Enriched expression and developmental regulation of the middle‐weight neurofilament (NF‐m) gene in song control nuclei of the zebra finch

Identification of Ser‐Pro and Thr‐Pro Phosphorylation Sites in Chicken Neurofilament‐M Tail Domain

Interaction of multiple nuclear proteins with the promoter region of the mouse 68‐kDa neurofilament gene

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