Isolation of the Arabidopsis ABI3 gene by positional cloning.

Giraudat, Jérôme; Hauge, Brian M.; Valon, Christiane; Smalle, Jan; Parcy, François; Goodman, Howard M.

doi:10.1105/tpc.4.10.1251

Cited by 895 publications

(715 citation statements)

References 35 publications

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“…Lesions in the genes encoding these factors underlie some of the phenotypic characteristics of mutants that are disrupted in seed maturation. For example, the VP1 gene of cereals and the ABI3 gene of Arabidopsis encode transcription factors that regulate the expression of ABA-responsive genes during seed development (for example, storage-protein genes and LEA genes) (McCarty and others 1991;Giraudat and others 1992). The encoded proteins belong to the B3 domain family of transcription factors; all ABI3/ VP1 proteins contain four conserved domains: an acidic activation domain and three basic domains, B1, B2, and B3.…”

Section: Aba Signaling Pathway Components: Putative Roles In Developmmentioning

confidence: 99%

Role of Abscisic Acid in Seed Dormancy

Kermode

2005

J Plant Growth Regul

249

160

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Seed dormancy is an adaptive trait that improves survival of the next generation by optimizing the distribution of germination over time. The agricultural and forest industries rely on seeds that exhibit high rates of germination and vigorous, synchronous growth after germination; hence dormancy is sometimes considered an undesirable trait. The forest industry encounters problems with the pronounced dormancy of some conifer seeds, a feature that can lead to non-uniform germination and poor seedling vigor. In cereal crops, an optimum balance is most sought after; some dormancy at harvest is favored because it prevents germination of the physiologically mature grain in the head prior to harvest (that is, preharvest sprouting), a phenomenon that leads to considerable damage to grain quality and is especially prominent in cool moist environments. The sesquiterpene abscisic acid (ABA) regulates key events during seed formation, such as the deposition of storage reserves, prevention of precocious germination, acquisition of desiccation tolerance, and induction of primary dormancy. Its regulatory role is achieved in part by cross-talk with other hormones and their associated signaling networks, via mechanisms that are largely unknown. Quantitative genetics and functional genomics approaches will contribute to the elucidation of genes and proteins that control seed dormancy and germination, including components of the ABA signal transduction pathway. Dynamic changes in ABA biosynthesis and catabolism elicit hormone-signaling changes that affect downstream gene expression and thereby regulate critical checkpoints at the transitions from dormancy to germination and from germination to growth. Some of the recent developments in these areas are discussed.

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Section: Aba Signaling Pathway Components: Putative Roles In Developmmentioning

confidence: 99%

Role of Abscisic Acid in Seed Dormancy

Kermode

2005

J Plant Growth Regul

249

160

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“…In addition to controlling embryo formation and seed maturation, these master regulators also repress the expression of genes required for the transition from embryonic to vegetative development (13)(14)(15)(16). Although the role of these master regulators during seed maturation is globally similar, some of their functions are very specific.…”

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confidence: 99%

Regulatory network analysis reveals novel regulators of seed desiccation tolerance in Arabidopsis thaliana

González-Morales

Montes

Hayano-Kanashiro

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

116

100

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Desiccation tolerance (DT) is a remarkable process that allows seeds in the dry state to remain viable for long periods of time that in some instances exceed 1,000 y. It has been postulated that seed DT evolved by rewiring the regulatory and signaling networks that controlled vegetative DT, which itself emerged as a crucial adaptive trait of early land plants. Understanding the networks that regulate seed desiccation tolerance in model plant systems would provide the tools to understand an evolutionary process that played a crucial role in the diversification of flowering plants. In this work, we used an integrated approach that included genomics, bioinformatics, metabolomics, and molecular genetics to identify and validate molecular networks that control the acquisition of DT in Arabidopsis seeds. Two DT-specific transcriptional subnetworks were identified related to storage of reserve compounds and cellular protection mechanisms that act downstream of the embryo development master regulators LEAFY COTYLEDON 1 and 2, FUSCA 3, and ABSCICIC ACID INSENSI-TIVE 3. Among the transcription factors identified as major nodes in the DT regulatory subnetworks, PLATZ1, PLATZ2, and AGL67 were confirmed by knockout mutants and overexpression in a desiccationintolerant mutant background to play an important role in seed DT. Additionally, we found that constitutive expression of PLATZ1 in WT plants confers partial DT in vegetative tissues.regulatory network | desiccation tolerance | drought tolerance | seed development | oligosaccharides D esiccation tolerance (DT) can be operationally defined as the ability of an organism to dry to equilibrium with moderately dry air (50 to 70% relative humidity at 20 to 30°C) and then resume normal function when rehydrated (1). DT organisms orchestrate a complex number of responses to protect cellular structures and prevent damage to proteins and nucleic acids. Early land plants evolved mechanisms to survive harsh drying environments to successfully exploit different ecosystems on land. Therefore, it has been postulated that the initial evolution of vegetative DT, in both vegetative and reproductive stages, was a crucial step required for the colonization of land by primitive plants of a fresh water origin (2).Seed DT, a trait that allows terrestrial plants to survive long periods of sparse water until favorable conditions are present for germination, is probably part of the answer to Darwin's "abominable mystery," the sudden appearance of great angiosperm diversity in the fossil record. In angiosperms, DT is acquired at the seed maturation stage, which involves a complex regulatory network (3, 4) that activates a large subset of genes involved in a number of mechanisms that influence seed survival in the dry state. The set of genes required for seed DT includes genes encoding protective proteins such as late embryogenesis abundant (LEA) (5, 6) and heat shock proteins (HSPs) (7), enzymes involved in scavenging reactive oxygen species (8) and the biosynthesis of protective compounds such as o...

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“…These results are consistent with other analysis showing that ectopic expression of LEC1, FUSCA3 (FUS3), and LEC2 genes induces the accumulation of lipid and protein reserves characteristic of developing seeds in cells of vegetative and reproductive tissues (Lotan et al, 1998;Stone et al, 2001Stone et al, , 2008Santos-Mendoza et al, 2005). Moreover, seed-specific overexpression of maize (Zea mays) LEC1 (ZmLEC1) resulted in an average 35% increase in seed oil but reduced germination and vegetative growth in the field (Shen et al, 2010) In Arabidopsis, the seed maturation program is controlled by a network of transcription factors that includes the B3 domain transcription factors (ABA-INSENSITIVE3 [ABI3], FUS3, and LEC2; Giraudat et al, 1992;Luerssen et al, 1998;Stone et al, 2001) and two LEC1-type HAP3 family CCAAT-binding factors, LEC1 and L1L (Lotan et al, 1998;Kwong et al, 2003). Given that these transcription factors have strong positive roles in promoting seed-specific programs, mechanisms have evolved to ensure that their expression is suppressed during vegetative growth.…”

Section: Discussionmentioning

confidence: 99%

Truncation of LEAFY COTYLEDON1 Protein Is Required for Asexual Reproduction in Kalanchoë daigremontiana

et al. 2014

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Kalanchoë daigremontiana reproduces asexually by generating numerous plantlets on its leaf margins. The formation of plantlets requires the somatic initiation of organogenic and embryogenic developmental programs in the leaves. However, unlike normal embryogenesis in seeds, leaf somatic embryogenesis bypasses seed dormancy to form viable plantlets. In Arabidopsis (Arabidopsis thaliana), seed dormancy and embryogenesis are initiated by the transcription factor LEAFY COTYLEDON1 (LEC1). The K. daigremontiana ortholog of LEC1 is expressed during leaf somatic embryo development. However, KdLEC1 encodes for a LEC1-type protein that has a unique B domain, with 11 unique amino acids and a premature stop codon. Moreover, the truncated KdLEC1 protein is not functional in Arabidopsis. Here, we show that K. daigremontiana transgenic plants expressing a functional, chimeric KdLEC1 gene under the control of Arabidopsis LEC1 promoter caused several developmental defects to leaf somatic embryos, including seed dormancy characteristics. The dormant plantlets also behaved as typical dormant seeds. Transgenic plantlets accumulated oil bodies and responded to the abscisic acid biosynthesis inhibitor fluridone, which broke somatic-embryo dormancy and promoted their normal development. Our results indicate that having a mutated form of LEC1 gene in K. daigremontiana is essential to bypass dormancy in the leaf embryos and generate viable plantlets, suggesting that the loss of a functional LEC1 promotes viviparous leaf somatic embryos and thus enhances vegetative propagation in K. daigremontiana. Mutations resulting in truncated LEC1 proteins may have been of a selective advantage in creating somatic propagules, because such mutations occurred independently in several Kalanchoë species, which form plantlets constitutively.

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Isolation of the Arabidopsis ABI3 gene by positional cloning.

Cited by 895 publications

References 35 publications

Role of Abscisic Acid in Seed Dormancy

Role of Abscisic Acid in Seed Dormancy

Regulatory network analysis reveals novel regulators of seed desiccation tolerance in Arabidopsis thaliana

Truncation of LEAFY COTYLEDON1 Protein Is Required for Asexual Reproduction in Kalanchoë daigremontiana

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