Isolation of taxol producing endophytic fungus Alternaria brassicicola from non-Taxus medicinal plant Terminalia arjuna

Gill, Harman; Vasundhara, M.

doi:10.1007/s11274-019-2651-8

Cited by 38 publications

(15 citation statements)

References 23 publications

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“…The results of the bioautography obtained in this study confirmed that the compounds revealed in TLC have antibacterial activity against the bacteria tested. These results are in agreement with other studies on chromatography and bioautography reported in the literature (Chen and Schwack, 2014), in which bioactive compounds were found in fungal extracts of Terminalia arjuna (Gill and Vasundhara, 2019). Similarly, more compounds with antibacterial activity were also isolated in endophytic fungi extracts of Helianthus annuus (Farhat et al, 2019).…”

Section: Discussionsupporting

confidence: 92%

Chromatography and bioautography of endophytic fungi extracts of Uncaria tomentosa (Willd.) DC with antibacterial activity

Gonã‡Alves¹,

ARAÃšJO²,

Brasil³

et al. 2019

J. Med. Plants Res.

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Endophytic fungi of different species have already proved their efficacy against a range of pathogenic bacteria. However, the separation of the compounds contained in these extracts that actually have an effective activity is not a constant, but it is essential for the scientific knowledge to formulate a new medication. Thus, this study aimed to separate compounds from two endophytic fungi extracts of Uncaria tomentosa with positive results against Gram-positive and Gram-negative bacteria. The separation was carried out by thin-layer chromatography and bioautography. For this, it was necessary to produce fungal extracts from the endophytes Colletotrichum (23916) and Fusarium (23952), which were separated from the liquid state by filtration. The remaining filtrate was divided into ethyl acetate and then concentrated in a rotary evaporator at 40°C. Antimicrobial activity was evaluated and the Minimum Inhibitory Concentration (MIC) was determined with the following test microorganisms: Escherichia coli, Enterecoccus faecalis, Klebsiella pneumoniae and Staphylococcus aureus. Subsequently, it was carried out the thin layer chromatography (TLC) of the extracts using hexane, ethyl acetate/hexane, ethyl acetate and acetate/methanol. The reading was performed in 312 nm UV light, and right after the chromatography, the bioautography was carried out. The following Rf values were obtained: 0.30 for K. pneumoniae and 0.35 for E. coli, in ethyl acetate, for the extract 23952, and 0.81 for K. pneumoniae and 0.41 for S. aureus, in styrene acetate, for the extract 23916, confirming different compounds by bioautography. Therefore, it was concluded that several compounds present in the extracts tested have antibacterial activity.

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Section: Discussionsupporting

confidence: 92%

Chromatography and bioautography of endophytic fungi extracts of Uncaria tomentosa (Willd.) DC with antibacterial activity

Gonã‡Alves¹,

ARAÃšJO²,

Brasil³

et al. 2019

J. Med. Plants Res.

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“…According to Zhu (Zhu and Chen, 2019), the extraction of paclitaxel from endophytic fungi has proven to be an effective alternative way to get the drug. Among endophytic fungi, several species, including Aspergillus fumigatus, Pestalotiopsis microspora, Alternaria brassicicola, are reported to produce this compound (Gill and Vasundhara, 2019;Kumar et al, 2019;Subban et al, 2019;Zhu and Chen, 2019), and our results indicate that Aspergillus sp. isolated from Brazil-nut tree also can.…”

Section: Lc-ms/ms Analysissupporting

confidence: 50%

Larvicidal and Oviposition Activity of Against Vector Aedes Aegypti and Molecular Docking Studies of Metabolites from the Crude Extract of the Endophytic Fungus Aspergillus Sp. Isolated from Bertholletia Excelsa Humn. & Bonpl.

Araújo

Marinho

Sena

et al. 2021

Preprint

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This work showed the crude extract of the endophytic fungus Aspergillus sp, isolated from the almonds of Bertholletia excelsa Humn & Bonlp collected in the Brazilian Amazon, oviposition deterrent, and larvicidal activity of against Aedes aegypti. In the oviposition deterrence test was observed that females able to lay eggs preferred the control oviposition sites (46.6%), suggesting the extract also could repel the oviposition. Futhermore, the extract showed larvicidal activity with LC50 26.86 µg/mL at 24 hours and 18.75 µg/mL at 48 hours. Molecular docking studies were carried out to elucidate the mechanism of action of the compounds identified against the enzyme acetylcholinesterase. The compound Aspergillol B was a potent larvicide with potential for inhibition for the acetylcholinesterase enzyme (-7.74 Kcal/mol). These unprecedented results reported indicate that the secondary metabolites obtained from crude extract of Aspergillus sp. present useful biological potential against vectors of public health importance and antibiotic-resistant bacteria.

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“…Compared with the monoculture pattern, the growth biomass of F. carica and T. cuspidata under interplanting pattern were significantly improved. It was reported that interplanting can effectively improve the growth of some plants [25,26]. F. carica could weaken the damage of strong light to T. cuspidata under IFT pattern, which could partly explain the promoting effect of F. carica on T. cuspidata growth under the interplanting pattern.…”

Section: Discussionmentioning

confidence: 98%

Enhancement of Interplanting of Ficus carica L. with Taxus cuspidata Sieb. et Zucc. on Growth of Two Plants

Yang

et al. 2021

Agriculture

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Medicinal-agroforestry systems are one of the multi-functional medicinal plant production systems, gaining attention as a sustainable alternative to traditional monoculture systems. In this study, three planting patterns were established which included: (1) monoculture F. carica (MF); (2) monoculture T. cuspidata (MT); and (3) interplanting F. carica with T. cuspidata (IFT). The differences of growth biomass, photosynthesis, soil nutrients, soil enzyme activities, soil microorganisms, and main secondary metabolites of F. carica and T. cuspidata under the above three models were investigated. Compared with the MF and MT patterns, IFT pattern for 5 months significantly increased the plant growth biomass, photosynthesis, soil organic carbon, total nitrogen, and secondary metabolites content. The activities of acid phosphatase, sucrase, protease, polyphenol oxidase, urease, dehydrogenase, and catalase in soil of IFT were significantly higher than MF and MT patterns. Results showed that IFT pattern is preferred compared to the MF and MT patterns. Our result will help to provide a feasible theoretical basis for the large-scale establishment of F. carica and T. cuspidata mixed forests and obtain high-quality medicine sources for extracting important active ingredients, psoralen and paclitaxel, which are crucial to the long-term sustainable development and production of medicinal plants.

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Isolation of taxol producing endophytic fungus Alternaria brassicicola from non-Taxus medicinal plant Terminalia arjuna

Cited by 38 publications

References 23 publications

Chromatography and bioautography of endophytic fungi extracts of Uncaria tomentosa (Willd.) DC with antibacterial activity

Chromatography and bioautography of endophytic fungi extracts of Uncaria tomentosa (Willd.) DC with antibacterial activity

Larvicidal and Oviposition Activity of Against Vector Aedes Aegypti and Molecular Docking Studies of Metabolites from the Crude Extract of the Endophytic Fungus Aspergillus Sp. Isolated from Bertholletia Excelsa Humn. & Bonpl.

Enhancement of Interplanting of Ficus carica L. with Taxus cuspidata Sieb. et Zucc. on Growth of Two Plants

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Isolation of taxol producing endophytic fungus Alternaria brassicicola from non-Taxus medicinal plant Terminalia arjuna

Cited by 38 publications

References 23 publications

Chromatography and bioautography of endophytic fungi extracts of Uncaria tomentosa (Willd.) DC with antibacterial activity

Chromatography and bioautography of endophytic fungi extracts of Uncaria tomentosa (Willd.) DC with antibacterial activity

Larvicidal and Oviposition Activity of Against Vector Aedes Aegypti and Molecular Docking Studies of Metabolites from the Crude Extract of the Endophytic Fungus Aspergillus Sp. Isolated from Bertholletia Excelsa Humn. &amp; Bonpl.

Enhancement of Interplanting of Ficus carica L. with Taxus cuspidata Sieb. et Zucc. on Growth of Two Plants

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Larvicidal and Oviposition Activity of Against Vector Aedes Aegypti and Molecular Docking Studies of Metabolites from the Crude Extract of the Endophytic Fungus Aspergillus Sp. Isolated from Bertholletia Excelsa Humn. & Bonpl.