Isolation of Streptococcus thermophilus and Lactobacillus delbrueckii as Starter Culture Candidate Originated from Indonesian Cow��s Milk

Andrian, Danish; Rizkinata, Denny; Susanto, Tan Steven Ryan; Lucy, Jap; Jan, Tan Tjie

doi:10.4014/mbl.1805.05011

Cited by 3 publications

(3 citation statements)

References 32 publications

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“…Akpinar et al [28] reported that two isolates among sixteen isolates of S. thermophilus had antibacterial activity against S. aureus and E.coli. However, the study conducted by Andrian et al [24] showed that S. thermophilus had the lowest antibacterial activity against E. coli and S. aureus. The inhibitory ability of our isolates of S. thermophilus in vitro against the tested multidrug-resistant bacteria, such as S. aureus ATCC29213, E. coli ATCC 25922, and E. coli ATCC 35218, con rms the production of antibacterial agents and may be predictive of good probiotics.…”

Section: Discussionmentioning

confidence: 98%

“…S. thermophilus is one of the predominant lactic acid bacteria, also classi ed among the genus of probiotic bacteria by their secretion of antimicrobial molecules and is an important component of many dairy starter cultures [24,25].…”

Section: Discussionmentioning

confidence: 99%

“…This test of sensibility toward antibiotics was performed according to the method described by Andrian et al [24] and Hamdaoui et al [42] with some modi cations. The isolates of S. thermophilus were examined against 20 antibiotics belonging to 8 families (Rifamycins, Aminosides, Phenicols, Beta-lactam, Glycopeptides, Clindamycin, Cotrimoxazole, and Cyclines).…”

Section: Antibiotic Susceptibility Testmentioning

confidence: 99%

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Probiotic properties and antibiotic susceptibility assessment of Streptococcus thermophilus isolates

Hamdaoui,

Azghar,

Benkirane

et al. 2023

Preprint

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Background Streptococcus thermophilus were considered one of probiotic bacterial strain; found in milk, cheese, and yogurt, that providing health benefits to humans. Due to their probiotic potentiality, S. thermophilus can be very helpful in replacing chemicals preservatives. This study came out to investigate the probiotic activity of ten S. thermophilus isolates obtained from raw cow milk in eastern Morocco against multidrug-resistant bacteria (MDR) bacteria: Staphylococcus aureus (ATCC 29213), Escherichia coli (ATCC: 25922 and 35218). Besides their antioxidant potential, bile salts tolerance as well as their antibiotic susceptibility, were evaluated Result The results showed remarkable in vitro inhibitory ability of the studied S. thermophilus isolates against the tested multidrug-resistant bacteria, as reported for S. thermophilus CFS and supernatant against Staphylococcus aureus (ATCC29213), confirming their potential as antibacterial agents. The mixtures of NaOH, catalase and proteinase K showed no inhibition zones against all tested multidrug-resistant bacteria; suggesting the main antibacterial effect of all isolates based on acids, hydrogen peroxide and/or proteins. For the antioxidant activity, the DPPH inhibition values varied between 18.97% and 44.44%, for the bacterial isolate ST9 and ST5, respectively. Notably, S. thermophilus isolates were able to survive up to 6h of incubation with a concentration of 0.1% of bile salt at 37°C. Thus, ST5 could be considered as the most promising bacterial isolate in terms of antioxidant activity. All isolates of S. thermophilus exhibited sensibility to all tested antibiotics except for rifampicin and trimethoprim-sulfamethoxazole compared to the standards proposed by the European committee on antimicrobial susceptibility testing. Conclusions The tested isolates were exhibited excellent antibacterial and potential antioxidant activities. In addition to that, these isolates were reported to resist rifampicin and trimethoprim-sulfamethoxazole antibiotics. These findings presage the use of S. thermophilus isolates as good probiotics.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Antibiotic Susceptibility Testmentioning

confidence: 99%

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Probiotic properties and antibiotic susceptibility assessment of Streptococcus thermophilus isolates

Hamdaoui,

Azghar,

Benkirane

et al. 2023

Preprint

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A novel therapeutic combination of dapagliflozin, Lactobacillus and crocin attenuates diabetic cardiomyopathy in rats: Role of oxidative stress, gut microbiota, and PPARγ activation

Khalaf

Hassan

El-Baz

et al. 2022

European Journal of Pharmacology

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Developing Starter Culture from Lactic Acid Bacteria Isolated from Cow Milk for the Production of Nigerian “Nunu”

Adedayo, M. R.,

Abdulkareem, T. O.

2024

AJASFR

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Starter cultures are mostly organisms deliberately introduced into food as a single culture or mixture of cultures to bring about fermentation and production of desired characteristics in food through metabolic and enzymatic activities. Organisms in this category have attained the generally regarded as safe (GRAS) status, are majorly lactic acid bacteria with food acidification and production of natural preservatives potential. This research was aimed at developing a starter culture for the production of local dairy drink “Nunu” Cow milk was obtained from “Gaa Mobolohunduro”, Tanke, Ilorin, Kwara State, Nigeria. Potential lactose fermenters were isolated on MRS agar in pour plate technique. The isolates were characterized and identified using standard procedures. Lactic Acid Bacteria isolated were inoculated as a starter culture for milk fermentation and the products were subjected to pH, titratable acid and organoleptic assessments following standard procedures. Two of the isolated lactic acid bacteria with the best fermentation products were identified through molecular technique. A total of six isolates were obtained and identified as Lactococcus sp., Leuconostoc sp., Pediococcus sp. and Lactobacillus spp. The pH ranges from 5.46±0.01 to 6.03±0.01while the titratable acidity ranged from 0.244±0.004 to 0.526±0.006. The moisture content ranged from 90.50±0.30 to 91.75±0.35. The fermentation product from the isolated Lactobacillus sp. scored 80 % during the organoleptic assessment. Molecular evidence confirmed the two isolates as Lactic Acid Bacteria. In conclusion, isolated species could be used as potential organisms for milk fermentation.

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Isolation of Streptococcus thermophilus and Lactobacillus delbrueckii as Starter Culture Candidate Originated from Indonesian Cow��s Milk

Cited by 3 publications

References 32 publications

Probiotic properties and antibiotic susceptibility assessment of Streptococcus thermophilus isolates

Probiotic properties and antibiotic susceptibility assessment of Streptococcus thermophilus isolates

A novel therapeutic combination of dapagliflozin, Lactobacillus and crocin attenuates diabetic cardiomyopathy in rats: Role of oxidative stress, gut microbiota, and PPARγ activation

Developing Starter Culture from Lactic Acid Bacteria Isolated from Cow Milk for the Production of Nigerian “Nunu”

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