Isolation of stem-like cells from spontaneous feline mammary carcinomas: Phenotypic characterization and tumorigenic potential

Barbieri, Federica; Würth, Roberto; Ratto, Alessandra; Campanella, Chiara; Vito, Guendalina; Thellung, Stefano; Daga, Antonio; Cilli, Michele; Ferrari, Angelo; Florio, Tullio

doi:10.1016/j.yexcr.2012.02.008

Cited by 26 publications

(31 citation statements)

References 69 publications

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“…Accordingly, these tumours may originate directly from epithelial cells that have undergone EMT and have therefore acquired CSCs properties, or from pre-existing CSCs expressing EMT-associated markers 8 . In FMC and human breast cancer cell lines, CSCs express high levels of CD133, CD44 and low (or none) CD24 30,68,69 . In this study, the immunohistochemical expression of CD24 and CD133 was not assessed due to the unavailability of the specific antibodies.…”

Section: Discussionmentioning

confidence: 99%

“…In vitro culture models are pivotal to understanding the role of EMT in the oncogenic process of FMC, determining the presence of possible subtypes and developing of new therapeutic approaches. However, the number of established FMC cell lines is still small 30,39,75–78,81–83 .…”

Section: Discussionmentioning

confidence: 99%

“…The overexpression of this cell surface protein leads to enhanced cell migration, cancer invasion and metastasis 28 . Additionally, a higher CD44 expression in combination with other surface markers is used for CSCs identification 29,30 .…”

Section: Introductionmentioning

confidence: 99%

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TiHo-0906: a new feline mammary cancer cell line with molecular, morphological, and immunocytological characteristics of epithelial to mesenchymal transition

Granados-Soler

Junginger

Hewicker‐Trautwein

et al. 2018

Sci Rep

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Feline mammary carcinomas (FMCs) with anaplastic and malignant spindle cells histologically resemble the human metaplastic breast carcinoma (hMBC), spindle-cell subtype. hMBCs display epithelial-to-mesenchymal transition (EMT) characteristics. Herein we report the establishment and characterization of a cell line (TiHoCMglAdcar0906; TiHo-0906) exhibiting EMT-like properties derived from an FMC with anaplastic and malignant spindle cells. Copy-number variations (CNVs) by next-generation sequencing and immunohistochemical characteristics of the cell line and the tumour were compared. The absolute qPCR expression of EMT-related markers HMGA2 and CD44 was determined. The growth, migration, and sensitivity to doxorubicin were assessed. TiHo-0906 CNVs affect several genomic regions harbouring known EMT-, breast cancer-, and hMBCs-associated genes as AKT1, GATA3, CCND2, CDK4, ZEB1, KRAS, HMGA2, ESRP1, MTDH, YWHAZ, and MYC. Most of them were located in amplified regions of feline chromosomes (FCAs) B4 and F2. TiHo-0906 cells displayed an epithelial/mesenchymal phenotype, and high HMGA2 and CD44 expression. Growth and migration remained comparable during subculturing. Low-passaged cells were two-fold more resistant to doxorubicin than high-passaged cells (IC50: 99.97 nM, and 41.22 nM, respectively). The TiHo-0906 cell line was derived from a poorly differentiated cellular subpopulation of the tumour consistently displaying EMT traits. The cell line presents excellent opportunities for studying EMT on FMCs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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TiHo-0906: a new feline mammary cancer cell line with molecular, morphological, and immunocytological characteristics of epithelial to mesenchymal transition

Granados-Soler

Junginger

Hewicker‐Trautwein

et al. 2018

Sci Rep

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“…Later on, another group also characterized CSC from FMC. These FMC-derived CSC showed in vitro self-renewal, long-lasting proliferation and in vivo tumorigenicity [76]. Immunofluorescence staining demonstrated that these feline CSC were positive for CD44, ER-alpha, and EGFR [76].…”

Section: Figmentioning

confidence: 99%

Mammary Stem Cell Research in Veterinary Science: An Update

Borena

Bussche

Burvenich

et al. 2013

Stem Cells and Development

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The mammary gland is an organ with a remarkable regenerative capacity that can undergo multiple cycles of proliferation, lactation, and involution. Growing evidence suggests that these changes are driven by the coordinated division and differentiation of mammary stem cell populations (MaSC). Whereas information regarding MaSC and their role in comparative mammary gland physiology is readily available in human and mice, such information remains scarce in most veterinary mammal species such as cows, horses, sheep, goats, pigs, and dogs. We believe that a better knowledge on the MaSC in these species will not only help to gain more insights into mammary gland (patho) physiology in veterinary medicine, but will also be of value for human medicine. Therefore, this review summarizes the current knowledge on stem cell isolation and characterization in different mammals of veterinary importance.

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“…It has been proposed that oophorectomy could block paracrine estrogen stimulation and early development of the ER negative progenitors. The protective effect of early ovariohysterectomy in cats, as discussed by Hughes and Dobson (2012), should be further analyzed in the context of cell lineage, since stem-like cells have recently been identified in feline mammary tumors (Barbiery et al, 2012). The design of treatment strategies that could benefit cats and humans alike might be facilitated by targeting luminal progenitor cells.…”

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confidence: 99%